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Effects of the inclusion of flaxseed and quercetin in the diet of fattening lambs on ruminal microbiota, in vitro fermentation and biohydrogenation of fatty acids

Published online by Cambridge University Press:  08 December 2015

S. ANDRÉS ,
R. BODAS ,
M. L. TEJIDO ,
F. J. GIRÁLDEZ ,
C. VALDÉS  and
S. LÓPEZ
S. ANDRÉS*
Affiliation:
Instituto de Ganadería de Montaña (CSIC-Universidad de León), Finca Marzanas s/n. 24346 Grulleros, León, Spain
R. BODAS
Affiliation:
Instituto Tecnológico Agrario – Subdirección de Investigación y Tecnología, Consejería de Agricultura y Ganadería, Junta de Castilla y León. Finca Zamadueñas. Ctra. Burgos, km 119. 47071 Valladolid, Spain
M. L. TEJIDO
Affiliation:
Instituto de Ganadería de Montaña (CSIC-Universidad de León), Finca Marzanas s/n. 24346 Grulleros, León, Spain
F. J. GIRÁLDEZ
Affiliation:
Instituto de Ganadería de Montaña (CSIC-Universidad de León), Finca Marzanas s/n. 24346 Grulleros, León, Spain
C. VALDÉS
Affiliation:
Instituto de Ganadería de Montaña (CSIC-Universidad de León), Finca Marzanas s/n. 24346 Grulleros, León, Spain
S. LÓPEZ
Affiliation:
Instituto de Ganadería de Montaña (CSIC-Universidad de León), Finca Marzanas s/n. 24346 Grulleros, León, Spain
*
*To whom all correspondence should be addressed. Email: [email protected]
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Summary

Thirty-two lambs (n = 8 per treatment) were fed a total mixed ration (TMR) formulated either with palm oil (CTRL; 34 g palm oil/kg TMR) or flaxseed (FS) (85 g FS/kg TMR) alone or enriched with quercetin (QCT, 34 g palm oil plus 2 g QCT/kg TMR; FS-QCT, 85 g FS plus 2 g QCT/kg TMR). The animals were slaughtered after being fed for at least 5 weeks with the experimental diets and samples of ruminal contents and ruminal liquid were collected for quantitative real-time polymerase chain reaction analyses of ruminal microbial species and fatty acid profile or in vitro fermentation, respectively. Results demonstrated that Butyrivibrio vaccenic acid (VA) and Butyrivibrio stearic acid (SA) producing bacteria copy numbers were decreased when FS was added to the diet of fattening lambs, which seemed to be in agreement with numerically (but not significantly) lower values for gas production, methane production and butyric acid during in vitro incubation. Ciliate protozoa were significantly enhanced by QCT, which was in accordance with numerically (but not significantly) higher values for in vitro methane production. Moreover, the modifications observed in ruminal microbial populations (Butyrivibrio and ciliate protozoa) when FS and QCT were included together (but not separately) in the diet of fattening lambs were probably related to a trend towards significantly higher values of rumenic acid (RA) in ruminal content. In conclusion, when FS and QCT were administered together in the diet of fattening lambs quantitative changes in the ruminal microbiota were observed, which might have promoted an increment of RA concentration in ruminal contents.

Type
Animal Research Papers
Information
The Journal of Agricultural Science , Volume 154 , Issue 3 , April 2016 , pp. 542 - 552
Copyright
Copyright © Cambridge University Press 2015 

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