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Effects of pantothenic acid and folic acid supplementation on total tract digestibility coefficient, ruminal fermentation, microbial enzyme activity, microflora and urinary purine derivatives in dairy bulls

Published online by Cambridge University Press:  22 November 2019

Z. Z. Wu ,
C. Wang ,
G. W. Zhang ,
Q. Liu Open the ORCID record for Q. Liu [Opens in a new window] ,
G. Guo ,
W. J. Huo ,
J. Zhang ,
Y. L. Zhang ,
C. X. Pei  and
S. L. Zhang
Z. Z. Wu
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi030801, P. R. China
C. Wang
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi030801, P. R. China
G. W. Zhang
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi030801, P. R. China
Q. Liu*
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi030801, P. R. China
G. Guo
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi030801, P. R. China
W. J. Huo
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi030801, P. R. China
J. Zhang
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi030801, P. R. China
Y. L. Zhang
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi030801, P. R. China
C. X. Pei
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi030801, P. R. China
S. L. Zhang
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi030801, P. R. China
*
Author for correspondence: Q. Liu, E-mail: liuqiangabc@163.com
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Abstract

The effects of pantothenic acid (PA) and folic acid (FA) addition on digestibility coefficient, ruminal fermentation and urinary purine derivative (PD) excretion in dairy bulls were evaluated. Eight rumen-cannulated Holstein dairy bulls were allocated to a replicated 4 × 4 Latin square design according to a 2 × 2 factorial arrangement. Diets were supplemented with two levels of FA (0 or 8.0 mg/kg dietary dry matter [DM]) and two of PA (0 or 60 mg/kg DM). The PA × FA interaction was not significant for all variables. Both supplements increased DM intake and average daily gain, but decreased a feed conversion ratio. Digestibility of DM, organic matter, crude protein and neutral detergent fibre increased, but ether extract digestibility was unchanged for both supplements. Digestibility of acid detergent fibre only increased with FA supplementation. For both supplements, ruminal pH and ammonia nitrogen (N) decreased, but total volatile fatty acid (VFA) concentration increased. Acetate proportion only increased with FA supplementation. Propionate proportion decreased for both supplements. Consequently, the acetate to propionate ratio increased. For both supplements, activity of xylanase and pectinase, population of Ruminococcus albus, R. flavefaciens, Fibrobacter succinogenes and Ruminobacter amylophilus and total PD excretion increased. Additionally, activity of carboxymethylcellulase, cellobiase, α-amylase and protease, and population of total bacteria, fungi, protozoa, methanogens, Butyrivibrio fibrisolvens and Prevotella ruminicola increased with FA addition. The results suggested that PA and FA supplementation stimulated ruminal microbial growth and enzyme activity, resulting in an increased digestibility coefficient and ruminal total VFA concentration in dairy bulls.

Keywords

Dairy bulldigestibility coefficientfolic acidmicrobial enzymemicroflorapantothenic acidruminal fermentation
Type
Animal Research Paper
Information
The Journal of Agricultural Science , Volume 157 , Issue 6 , August 2019 , pp. 555 - 562
Copyright
Copyright © Cambridge University Press 2019

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