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Effects of maternal vitamin D3 during pregnancy on FASN and LIPE mRNA expression in offspring pigs

Published online by Cambridge University Press:  27 March 2020

Liping Guo ,
Zhiguo Miao Open the ORCID record for Zhiguo Miao [Opens in a new window] ,
Hanjun Ma  and
Sergiy Melnychuk
Liping Guo
Affiliation:
Sumy National Agrarian University, Sumy, Ukraine School of Food Science and Technology, Henan Institute of Science and Technology, Xinxiang, Henan, 453003, P. R. China
Zhiguo Miao*
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, Henan, 453003, P. R. China
Hanjun Ma
Affiliation:
School of Food Science and Technology, Henan Institute of Science and Technology, Xinxiang, Henan, 453003, P. R. China
Sergiy Melnychuk
Affiliation:
Sumy National Agrarian University, Sumy, Ukraine
*
Author for correspondence: Zhiguo Miao, E-mail: [email protected]
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Abstract

In this study, sows were fed 200 (LD), 800 (ND) and 3200 (HD) IU of vitamin D3/kg basal diet during pregnancy (from 41 d to birth), respectively. All their offspring pigs were fed the same vitamin D3 replete die. At 150 days of age, a total of 18 offspring pigs (six offspring pigs per maternal diet group, sex balance) were weighed and slaughtered to investigate effects of maternal vitamin D3 during pregnancy on fatty acids synthase (FASN) and hormone-sensitive lipase (LIPE) expression in offspring pigs. The results showed that LD offspring pigs had higher FASN mRNA expression and the ratio of FASN/LIPE mRNA expression in subcutaneous adipose tissue, as well as higher LIPE mRNA expression of longissimus dorsal muscle, whereas, had lower the ratio of FASN/LIPE mRNA expression in longissimus dorsal muscle compared with ND or HD offspring pigs, respectively. Meanwhile, LD offspring pigs had higher carcass fat, average backfat thickness (ABFT), serum insulin and leptin levels, lower intramuscular fat (IMF), serum free fatty acid and triglycerol levels compared with ND or HD offspring pigs. In addition, the ratio of FASN/LIPE mRNA expression was negatively correlated with IMF content, and positively correlated to carcass fat content and ABFT in offspring pigs. Meanwhile, FASN mRNA expression was positively correlated with carcass fat content, while negatively correlated with ABFT in offspring pigs. These results suggested that maternal vitamin D3 affected fat accumulation and meat quality by regulating FASN and LIPE mRNA expression in offspring pigs.

Keywords

Adipogenesisgene expressionoffspring pigspregnancyvitamin D3
Type
Animal Research Paper
Information
The Journal of Agricultural Science , Volume 158 , Issue 1-2 , March 2020 , pp. 128 - 135
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Copyright
Copyright © Cambridge University Press 2020

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Footnotes

*

These two authors contributed equally to this work.

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