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The effects of fasting on the concentrations of intermediate metabolites in the blood and hepatic tissues of pregnant and non-pregnant ewes

Published online by Cambridge University Press:  27 March 2009

I. D. Herriman  and
R. J. Heitzman
I. D. Herriman
Affiliation:
Department of Functional Pathology, Agricultural Besearch Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire RG16 0NN
R. J. Heitzman
Affiliation:
Department of Functional Pathology, Agricultural Besearch Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire RG16 0NN
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Summary

Blood and liver samples were taken from four fasted pregnant and five fasted non-pregnant Finnish Landrace × Dorset Horn ewes. The following metabolites were measured: adenine monophosphate (AMP), adenine diphosphate (ADP), adenine triphosphate (ATP), citrate, 2-oxoglutarate, phosphoenolpyruvate, pyruvate, lactate, 2-phosphoglycerate, 3-phosphoglycerate, α-glycerophosphate, malate, β-hydroxybutyrate, acetoacetate, glutamate, alanine and NAD+.

The effects of a 6-day fast on non-pregnant ewes were shown as significant reductions in the hepatic concentrations of 2-phosphoglycerate, alanine, NAD+ and ADP, together with significant increases in the hepatic concentrations of 3-hydroxybutyrate, malate and the ratio of 3-hydroxybutyrate:acetoacetate. In the blood there were significant reductions in the concentrations of urea, citrate and in the ratio of 3-hydroxybutyrate: acetoacetate.

Similar but more marked changes occurred in the late pregnant ewes, with additional significant reductions in the hepatic concentrations of citrate, ATP and the level of cell energy, and additional increases in the blood of the ratio of lactate: pyruvate and the concentrations of 3-hydroxybutyrate and 2-oxoglutarate. However, unlike the non-pregnant ewes no significant changes in either the plasma concentration of urea or the hepatic concentration of ADP were observed in the late pregnant ewes.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 90 , Issue 3 , June 1978 , pp. 579 - 585
Copyright
Copyright © Cambridge University Press 1978

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