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Effects of alpha-lipoic acid on productive performance, immunity, antioxidation and serum metabolism in Holstein cows

Published online by Cambridge University Press:  10 December 2024

Hongrui Zhang Open the ORCID record for Hongrui Zhang [Opens in a new window] ,
Zhiguo Jiang Open the ORCID record for Zhiguo Jiang [Opens in a new window] ,
Tong Fu ,
Linfeng Wang ,
Gaiying Li ,
Hongxia Lian ,
Liyang Zhang  and
Tengyun Gao
Hongrui Zhang*
Affiliation:
College of Animal Science and Technology, Ningxia University, Yingchuan, 750021, P. R. China
Zhiguo Jiang
Affiliation:
College of Animal Science and Technology, Ningxia University, Yingchuan, 750021, P. R. China
Tong Fu
Affiliation:
Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, No.15 Longzihu University Area, Zhengdong New District, Zhengzhou, 450046, P. R. China
Linfeng Wang
Affiliation:
Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, No.15 Longzihu University Area, Zhengdong New District, Zhengzhou, 450046, P. R. China
Gaiying Li
Affiliation:
Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, No.15 Longzihu University Area, Zhengdong New District, Zhengzhou, 450046, P. R. China
Hongxia Lian
Affiliation:
Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, No.15 Longzihu University Area, Zhengdong New District, Zhengzhou, 450046, P. R. China
Liyang Zhang
Affiliation:
Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, No.15 Longzihu University Area, Zhengdong New District, Zhengzhou, 450046, P. R. China
Tengyun Gao*
Affiliation:
Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, No.15 Longzihu University Area, Zhengdong New District, Zhengzhou, 450046, P. R. China
*
Corresponding authors: Hongrui Zhang; Email: [email protected]; Tengyun Gao; Email: [email protected]
Corresponding authors: Hongrui Zhang; Email: [email protected]; Tengyun Gao; Email: [email protected]
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Abstract

The aim of this study was to investigate the antioxidant effect of alpha-lipoic acid (α-LA) in dairy cows and its metabolic mechanism. Thirty Holstein cows weighing 550 ± 25 kg, 200 ± 15 days of lactation and calving 2–3 times were randomly divided into three groups, ten cows in each group. Different doses of α-LA were added based on body weight: 0 (CTL), 30 (LA-L) and 60 (LA-H) mg/kg per head per day; 7 days adaptation period, 30 days formal period. Milk production was recorded daily during the test period. Milk and blood samples were collected on the last day. ELISA kits and automatic biochemical analyser were used to detect the indicators in blood; serum metabolites were detected and analysed by non-target metabolomics. The results of the study showed that the addition of α-LA significantly increased milk yield; blood concentrations for HDL, triglyceride, cortisol and triiodothyronine were significantly elevated; and levels of glutathione reductase and nitric oxide synthase were significantly reduced in LA-L group as compared to CTL group. The concentrations of IL-1β, IL-2, TNF-α, IgG and IgA were significantly higher after supplementation with α-LA. Metabolomics analysis revealed 13 and 15 differential metabolites each in positive or negative modes. Methylmalonic acid levels were significantly higher following α-LA supplementation compared to CTL group, as were D-lactose, D-maltose and oleanolic acid levels in LA-L group. In summary, α-LA can enhance milk production, improve antioxidant capacity and immunity, and is more beneficial for animal production and economic benefits at 30 mg/kg.

Keywords

alpha-lipoic acidantioxidantdairy cowmilk productionserum metabolomics
Type
Animal Research Paper
Information
The Journal of Agricultural Science , Volume 162 , Issue 6 , December 2024 , pp. 629 - 637
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Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press

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