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The effect of interval between harvests and nitrogen application on the proportion and digestibility of cell wall, cellulose, hemicellulose and lignin and on the proportion of lignified tissue in leaf cross-section in two perennial ryegrass varieties

Published online by Cambridge University Press:  27 March 2009

D. Wilman ,
M. Daly ,
A. Koocheki  and
A. B. Lwoga
D. Wilman
Affiliation:
Department of Agriculture, University College of Wales, Aberystwyth
M. Daly
Affiliation:
Department of Agriculture, University College of Wales, Aberystwyth
A. Koocheki
Affiliation:
Department of Agriculture, University College of Wales, Aberystwyth
A. B. Lwoga
Affiliation:
Department of Agriculture, University College of Wales, Aberystwyth
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Summary

The effect of six intervals between harvests and two levels of N application on the proportion and digestibility of cell wall was studied in two perennial ryegrass (Lolium perenne) varieties in the first and second harvest years in a field experiment. In the oase of four of the six intervals between harvests the proportion and digestibility of cellulose, hemicellulose and lignin and the N content of cell wall were determined. Diets derived from three of the harvesting treatments were fed, at maintenance level, to sheep as a partial in vivo check on the in vitro procedures. The effect of six intervals between harvests and two levels of N application on the proportion of lignified tissue in leaf cross-section was determined in two varieties in the first harvest year.

Effects of interval between harvests and time of year on herbage digestibility were due to effects on both proportion and digestibility of cell wall, particularly the latter. Hemicellulose digestibility appeared to be rather more affected by interval between harvests and rather more closely associated with the proportion of lignin than was cellulose digestibihty. The proportion of cell wall was particularly low and its digestibility particularly high in April and early May.

The N in cell wall accounted for about 10% of total N in harvested herbage on all treatments. The ratio of cellulose to hemicellulose averaged 1:0·88 and hemicellulose was rather more digestible than cellulose. Cellulose and cell wall were less digestible in vitro than in vivo.

The application of N tended to reduce the proportion of cell wall and to increase its digestibility in leafy but not in stemmy crops.

Interval between harvests and N application had no effect on the proportion of lignified tissue in leaf cross-section.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 89 , Issue 1 , August 1977 , pp. 53 - 63
Copyright
Copyright © Cambridge University Press 1977

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References

Agricultural Research Council (1965). The Nutrient Requirements of Farm Livestock. No. 2. Ruminants, pp. 264. London: Agricultural Research Council.Google Scholar
Bailey, R. W. & Ulyatt, M. J. (1970). Pasture quality and ruminant nutrition. II. Carbohydrate and lignin composition of detergent-extracted residues from pasture grasses and legumes. New Zealand Journal of Agricultural Research 13, 591–604.CrossRefGoogle Scholar
Clancy, M. J. & Wilson, R. K. (1966). Development and application of a new chemical method for predicting the digestibility and intake of herbage samples. Proceedings Xth International Grassland Congress, Helsinki, 445–53.Google Scholar
Daly, M. (1976). Agronomic studies on the structure of grasses, with reference to nutritive value. M.Sc. thesis, University College of Wales, Aberystwyth.Google Scholar
Deinum, B. (1974). Structural inhibitors of quality in forage. Proceedings of the 5th General Meeting of the European Grassland Federation, Uppsala, 1973. Main papers. Växtodling 28, 42–51.Google Scholar
Jarrige, R. & Minson, D. J. (1964). Digestibilité des constituents du ray-grass anglais S.24 et du dactyle S.37, plus spécialement des constituants glucidiques. Annales de Zootechnie 13, 117–50.CrossRefGoogle Scholar
Jones, D. I. H. & Bailey, R. W. (1972). The hydrolysis of cell wall polysaccharides from freeze-dried and oven-dried herbage by rumen and mould carbohydrases. Journal of the Science of Food and Agriculture 23, 609–14.CrossRefGoogle ScholarPubMed
Jones, D. I. H. & Bailey, R. W. (1974). Hydrolysis of the cell-wall carbohydrates of grasses by carbohydrases in relation to voluntary intake by sheep. Journal of Agricultural Science, Cambridge 83, 105–11.CrossRefGoogle Scholar
Koocheki, A. (1975). Aspects of plant structure, nutritive value and productivity in forage, particularly Lolium perenne. Ph.D. thesis, University College of Wales, Aberystwyth.Google Scholar
Lwoga, A. B. (1975). Digestibility and productivity studies in Lolium perenne. Ph.D. thesis, University College of Wales, Aberystwyth.Google Scholar
Moir, K. W. (1974). The constancy of the digested cell wall in grass. Journal of Agricultural Science, Cambridge 83, 295–8.CrossRefGoogle Scholar
Osbourn, D. F., Terry, R. A., Outen, G. E. & Cammell, S. B. (1974). The significance of a determination of cell walls as the rational basis for the nutritive evaluation of forages. Proceedings XIIth International Grassland Congress, Moscow, vol. III, 374–80.Google Scholar
Osbourn, D. F., Cammell, S. B., Terry, R. A. & Outen, G. E. (1975). Forage composition and the conduct of digestion trials for the comparative evaluation of laboratory procedures. Journal of the British Grassland Society 30, 101–5.CrossRefGoogle Scholar
Raymond, W. F. (1969). The nutritive value of forage crops. Advances in Agronomy 21, 1–108.CrossRefGoogle Scholar
Sant, F. I. (1969). A comparison of the morphology and anatomy of seedling leaves of Lolium multiflorum Lam. and L. perenne L. Annals of Botany New Series 33, 303–13.CrossRefGoogle Scholar
Tilley, J. M. A. & Terry, R. A. (1963). A two-stage technique for the in vitro digestion of forage crops. Journal of the British Grassland Society 18, 104–11.CrossRefGoogle Scholar
Van Soest, P. J. (1963). Use of detergents in the analysis of fibrous feeds. II. A rapid method for the determination of fiber and lignin. Journal of the Association of Official Agricultural Chemists 46, 829–35.Google Scholar
Van Soest, P. J. (1967). Development of a comprehensive system of feed analyses and its application to forages. Journal of Animal Science 26, 119–28.CrossRefGoogle Scholar
Van Soest, P. J. & Wine, R. H. (1967). Use of detergents in the analysis of fibrous feeds. IV. Determination of plant cell-wall constituents. Journal of the Association of Official Analytical Chemists 50, 50–5.Google Scholar
Van Soest, P. J. & Wine, R. H. (1968). Determination of lignin and cellulose in acid-detergent fiber with permanganate. Journal of the Association of Official Analytical Chemists 51, 780–5.Google Scholar
Van Soest, P. J., Wine, R. H. & Moore, L. A. (1966). Estimation of the true digestibility of forages by the in vitro digestion of cell walls. Proceedings Xth International Grassland Congress, Helsinki, 438–41.Google Scholar
Waite, R., Johnston, , Margaret, J. & Armstrong, D. G. (1964). The evaluation of artificially dried grass as a source of energy for sheep. I. The effect of stage of maturity on the apparent digestibility of rye-grass, cocksfoot and timothy. Journal of Agricultural Science, Cambridge 62, 391–8.CrossRefGoogle Scholar
Wilman, D., Dreoushiotis, D., Koocheki, A., Lwoga, A. B. & Shim, J. S. (1976 a). The effect of interval between harvests and nitrogen application on the proportion and yield of crop fractions in four ryegrass varieties in the first harvest year. Journal of Agricultural Science, Cambridge 86, 189–203.CrossRefGoogle Scholar
Wilman, D., Droushiotis, D., Koocheki, A., Lwoga, A. B. & Shim, J. S. (1976 b). The effect of interval between harvests and nitrogen application on the digestibility and digestible yield and nitrogen content and yield of four ryegrass varieties in the first harvest year. Journal of Agricultural Science, Cambridge 86, 393–9.CrossRefGoogle Scholar
Wilman, D., Droushiotis, D., Mzamane, Mary N. & Shim, J. S. (1977). The effect of interval between harvests and nitrogen application on initiation, emergence and longevity of leaves, longevity of tillers and dimensions and weights of leaves and ‘stems’ in Lolium. Journal of Agricultural Science, Cambridge 89, 65–79.CrossRefGoogle Scholar
Wilman, D., Koocheki, A. & Lwoga, A. B. (1976c). The effect of interval between harvests and nitrogen application on the proportion and yield of crop fractions and on the digestibility and digestible yield and nitrogen content and yield of two perennial ryegrass varieties in the second harvest year. Journal of Agricultural Science, Cambridge 87, 59–74.CrossRefGoogle Scholar
Wilman, D., Koocheki, A., Lwoga, A. B., Droushiotis, D. & Shim, J. S. (1976 d). The effect of interval between harvests and nitrogen application on the numbers and weights of tillers and leaves in four ryegrass varieties. Journal of Agricultural Science, Cambridge 87, 45–57.CrossRefGoogle Scholar