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The effect of energy and protein intake on the excretion of purine derivatives

Published online by Cambridge University Press:  27 March 2009

P. Susmel ,
B. Stefanon ,
E. Plazzotta ,
M. Spanghero  and
C. R. Mills
P. Susmel
Affiliation:
Department of Animal Production Science, Faculty of Agriculture, University of Vdine, via San Mauro 2, 33010 Pagnacco, Udine, Italy
B. Stefanon
Affiliation:
Department of Animal Production Science, Faculty of Agriculture, University of Vdine, via San Mauro 2, 33010 Pagnacco, Udine, Italy
E. Plazzotta
Affiliation:
Department of Animal Production Science, Faculty of Agriculture, University of Vdine, via San Mauro 2, 33010 Pagnacco, Udine, Italy
M. Spanghero
Affiliation:
Department of Animal Production Science, Faculty of Agriculture, University of Vdine, via San Mauro 2, 33010 Pagnacco, Udine, Italy
C. R. Mills
Affiliation:
Department of Animal Production Science, Faculty of Agriculture, University of Vdine, via San Mauro 2, 33010 Pagnacco, Udine, Italy
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Summary

In three separate experiments, groups of four, three and six mature, rumen-cannulated Simmental cows were fed diets designed to supply different quantities of energy (E) and nitrogen (N) to the rumen microbes. Experiment 1 (straw plus concentrate plus urea) had balanced E and N supplies; Expt 2 (hay and four levels of soyabean meal) had different sub-optimum N levels at the same intake as Expt 1; and Expt 3 (hay plus maize plus two levels of urea) reproduced the N shortage in Expt 2 at a higher level of intake.

The amount of total N excreted in the urine (TUN) was 574 mg/kg LW0·75 in Expt 1 and 420 mg/kg LW0·75 of this N was excreted as total purine nitrogen (TPN). In Expt 2, TUN increased significantly (P < 0·05) with increasing soyabean levels; TPN also increased, reaching the level observed in Expt 1 when soyabean meal supplementation was highest. In Expt 3, TUN and TPN increased with increasing dietary urea concentrations; TUN and TPN were always higher than in Expts 1 and 2.

Estimated microbial nitrogen supply (EMNS, based on TPN) was always considerably lower than estimates based on fermentable non-protein OM and crude protein (EMNR-EN) or an assumed yield of microbial N per kg OM apparently digested in the rumen (EMNR-OM). Regression analyses of EMNS on EMNR-EN, EMNR-OM or OM intake had moderate r2 values (0·76, 0·65 and 0·62 respectively) but the constant terms were significantly different from zero. The regressions of EMNR-EN and EMNR-OM on TPN gave angular coefficients of 15·81 and 11·47 respectively.

The correlation between rumen liquid parameters (total count, bacterial DM, ATP and nitrogen) and OM intake, EMNS or EMNR increased with sampling time (from 09.00 to 16.00 h). The EMNR-OM produced correlation coefficients similar to those obtained with OM intake; these correlations were numerically higher than those obtained with EMNS or EMNR-EN.

Type
Animals
Information
The Journal of Agricultural Science , Volume 123 , Issue 2 , October 1994 , pp. 257 - 265
Copyright
Copyright © Cambridge University Press 1994

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References

Agricultural Research Council (1980). The Nutrient Requirements of Ruminant Livestock. Slough: Commonwealth Agricultural Bureaux.Google Scholar
Agricultural Research Council (1984). The Nutrient Requirements of Ruminant Livestock, Supplement No. 1. Slough: Commonwealth Agricultural Bureaux.Google Scholar
Arambel, M. J., Bartley, E. E., Dufva, G. S., Nagaraja, T. G. & Dayton, A. D. (1982). Effect of diet on amino and nucleic acids of rumen bacteria and protozoa. Journal of Dairy Science 65, 2095–2101.CrossRefGoogle ScholarPubMed
Association of Official Analytical Chemists (1984). Official Methods of Analysis, 14th edition. Washington DC: AOAC.Google Scholar
Bates, D. B., Gillett, J. A., Barao, S. A. & Bergen, W. G. (1985). The effect of specific growth rate and stage of growth on nucleic acid-protein values of pure cultures and mixed ruminal bacteria. Journal of Animal Science 61, 713–724.CrossRefGoogle Scholar
Blaxter, K. L. & Clapperton, J. L. (1965). Prediction of the amount of methane produced by ruminants. British Journal of Nutrition 19, 511–522.CrossRefGoogle ScholarPubMed
Cecava, M. J., Merchen, N. R., Gay, L. C. & Berger, L. L. (1990). Composition of ruminal bacteria harvested from steers as influenced by dietary energy level, feeding frequency, and isolation techniques. Journal of Dairy Science 73, 2480–2488.CrossRefGoogle ScholarPubMed
Chen, X. B., Hovell, F. D. DeB. & Ørskov, E. R. (1990 a). Excretion of purine derivatives by ruminants: recycling of allantoin into the rumen via saliva and its fate in the gut. British Journal of Nutrition 63, 197–205.CrossRefGoogle ScholarPubMed
Chen, X. B., Ørskov, E. R. & Hovell, F. D. DeB. (1990 b). Excretion of purine derivatives by ruminants: endogenous excretion, differences between cattle and sheep. British Journal of Nutrition 63, 121–129.CrossRefGoogle ScholarPubMed
Chen, X. B., Hovell, F. D. DeB., Ørskov, E. R. & Brown, D. S. (1990 c). Excretion of purine derivatives by ruminants: effect of exogenous nucleic acid supply on purine derivative excretion by sheep. British Journal of Nutrition 63, 131–142.CrossRefGoogle ScholarPubMed
Chen, X. B., Ørskov, E. R. & Hovell, F. D. DeB. (1991). The use of intragastric infusion in studies on excretion of purine derivatives as a measure of microbial protein supply in ruminants. In Proceedings of the 6th International Symposium on Protein Metabolism and Nutrition, Herning, Denmark (Eds Eggum, E. O., Boisen, S., Borsting, C., Danfaer, A., Hvelplund, T.), Vol. 2, pp. 67–70. Foulum, Denmark: National Institute of Animal Science.Google Scholar
Chen, X. B., Chen, Y. K., Franklin, M. F., Ørskov, E. R. & Shand, W. J. (1992). The effect of feed intake and body weight on purine derivative excretion and microbial protein supply in sheep. Journal of Animal Science 70, 1534–1542.CrossRefGoogle ScholarPubMed
Dewhurst, R. J., Waters, C. J. & Webster, A. J. F. (1988). Assessment of the energetic efficiency of rumen microbial protein yield using allantoin excretion. In Proceedings of the 5th International Symposium on Protein Metabolism and Nutrition, Rostock. EAAP publication No. 35. p. 50.Google Scholar
Dhanoa, M. S., Siddons, R. C., France, J. & Gale, D. L. (1985). A multicompartmental model to describe marker excretion patterns in ruminant faeces. British Journal of Nutrition 53, 663–671.CrossRefGoogle ScholarPubMed
Erfle, J. D., Mahadevan, S. & Sauer, F. D. (1979). Effect of diet quality on adenosine 5′-triphosphate concentration and adenylate energy charge of rumen microbes from fistulated cows. Journal of Dairy Science 62, 284–291.CrossRefGoogle Scholar
Forsberg, C. W. & Lam, K. (1977). Use of adenosine-5′-triphosphate as an indicator of the microbiota biomass in rumen contents. Applied and Environmental Microbiology 33, 528–537.CrossRefGoogle ScholarPubMed
Fujihara, T., Ørskov, E. R., Reeds, P. J. & Kyle, D. J. (1987). The effect of protein infusion on urinary excretion of purine derivatives in ruminants nourished by intragastric nutrition. Journal of Agricultural Science, Cambridge 109, 7–12.CrossRefGoogle Scholar
Ganev, G., Ørskov, E. R. & Smart, R. (1979). The effect of roughage or concentrate feeding and rumen retention time on total degradation of protein in the rumen. Journal of Agricultural Science, Cambridge 93, 651–656.CrossRefGoogle Scholar
Hungate, R. E. (1966). The Rumen and its Microbes. New York: Academic Press.Google Scholar
Institut National de la Recherche Agronomique (1978). Alimentation des Ruminants. Versailles: INRA Publications.Google Scholar
Institut National de la Recherche Agronomique (1988). Alimentation des Bovins, Ovins et Caprins. Versailles: INRA Publications.Google Scholar
Lindberg, J. E. & Jacobsson, K.-G. (1990). Nitrogen and purine metabolism at varying energy and protein supplies in sheep sustained on intragastric infusion. British Journal of Nutrition 64, 359–370.CrossRefGoogle ScholarPubMed
Ørskov, E. R. & McDonald, I. (1979). The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of Agricultural Science, Cambridge 92, 499–503.CrossRefGoogle Scholar
Statistical Analysis System (1988). SAS/STAT User's Guide, Release 6·03 Edition. Cary, NC: SAS Institute Inc.Google Scholar
Stefanon, B. & Ovan, M. (1988). Impiego di bicromato sodico per la valutazione della velocità di transito ruminale degli alimenti. Zootecnica e Nutrizione Animate 15, 431–436.Google Scholar
Street, J.C., Butcher, J. E. & Harris, L. E. (1964). Estimating urine energy from urine nitrogen. Journal of Animal Science 23, 1039–1041.CrossRefGoogle Scholar
Susmel, P. & Stefanon, B. (1987). Sistema per la valutazione delle proteine alimentari per i ruminanti. Zootecnica e Nutrizione Animale 13, 567–582.Google Scholar
Susmel, P., Stefanon, B., Mills, C. R. & Colitti, M. (1991). Determinazione dell'adenosin-′5-trifosfato in bioluminescenza e suo impiego come indicatore della biomassa batterica nel liquido ruminale. Zootecnica e Nutrizione Animate 17, 227–238.Google Scholar
Susmel, P., Plazzotta, E., Mills, C. R. & Stefanon, B. (1993). Determination of RNA and ATP in the rumen liquid of cows fed with diets differing in forage to concentrate ratio. Journal of the Science of Food and Agriculture 63, 39–45.CrossRefGoogle Scholar
Techinical Committee on Responses to Nutrients (1992). Nutrient requirements of ruminant animals: protein. Nutrition Abstracts and Reviews 62, 787–835.Google Scholar
Topps, J. H. & Elliott, R. C. (1965). Relationship between concentrations of ruminal nucleic acids and excretion of purine derivatives by sheep. Nature 205, 498–499.CrossRefGoogle Scholar
Verbic, J., Chen, X. B., MacLeod, N. A. & Ørskov, E. R. (1990). Excretion of purine derivatives by ruminants. Effect of microbial nucleic acid infusion on purine derivative excretion by steers. Journal of Agricultural Science, Cambridge 114, 243–248.CrossRefGoogle Scholar
Williams, C. H., David, D. J. & Iismaa, O. (1962). The determination of chromic oxide in faeces samples by atomic absorption spectrophotometry. Journal of Agricultural Science 59, 381–385.CrossRefGoogle Scholar
Wolstrup, J. & Jensen, K. (1976). Adenosine triphosphate in the bovine rumen during maximum nutrient supply and starvation. Journal of Applied Bacteriology 41, 243–250.CrossRefGoogle Scholar