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Developmental changes in the biochemical composition of foetal and neonatal pig muscle with special reference to DNA synthesis

Published online by Cambridge University Press:  27 March 2009

Linda J. Farmer ,
W. S. Mackie  and
P. J. Ritchie
Linda J. Farmer
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
W. S. Mackie
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
P. J. Ritchie
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
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Summary

DNA and protein concentrations were measured in selected muscles from foetal and neonatal pigs; the protein: DNA ratios of hind-limb muscles were similar to those of the forelimb from 83 days gestation to 27 days after birth. The ratios increased during the perinatal period, providing evidence that maturation of muscle began in the last few days of pregnancy. RNA concentration, cathepsin D activity and tritiated thymidine incorporation were measured in muscles from the neonatal animals and the results indicated a surge of biosynthetic activity in the first days of life. Values obtained from the hind- and forelimb muscles were similar throughout the period of study with RNA: DNA ratio, cathepsin D activity and thymidine incorporation reaching maximum values at 4 days of age. A considerable proportion of thymidine incorporation was attributed to the mitotic activity of satellite cells.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 95 , Issue 3 , December 1980 , pp. 563 - 574
Copyright
Copyright © Cambridge University Press 1980

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