Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-30T15:56:27.486Z Has data issue: false hasContentIssue false
  • English
  • Français

Culture and fertilization of sheep ovarian oocytes I. Effect of culture medium on resumption of meiosis

Published online by Cambridge University Press:  27 March 2009

J. F. Quirke  and
I. Gordon
J. F. Quirke
Affiliation:
Faculty of Agriculture, University College, Dublin
I. Gordon
Affiliation:
Faculty of Agriculture, University College, Dublin
Get access Rights & Permissions [Opens in a new window]

Summary

Oocyte recovery was conducted in 329 slaughtered sheep; from 3072 vesicular follicles ruptured, 1210 oocytes were obtained (av. 3·7 per sheep). After recovery or culture in various media, 801 oocytes were examined for developmental stages. In cattle follicular fluid, little evidence of resumption of meiosis was found. In sheep follicular fluid, meiosis was resumed in many oocytes, but was arrested at the pro-metaphase to metaphase-I stage. A high proportion of oocytes matured to metaphase-II when cultured in growth medium.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 76 , Issue 3 , June 1971 , pp. 369 - 372
Copyright
Copyright © Cambridge University Press 1971

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Chang, M. C. (1955). The maturation of rabbit ooeytes in culture and their maturation, activation, fertilization and subsequent development in the Fallopian tubes. J. exp. Zool. 123, 379–405.CrossRefGoogle Scholar
Dziuk, P. J. (1965). Timing of maturation and fertilization of the sheep egg. Anat. Rec. 153, 211–23.CrossRefGoogle ScholarPubMed
Edwards, R. G. (1965). Maturation in vitro of mouse, sheep, cow, pig, rhesus monkey and human ovarian oocytes. Nature, Lond. 208, 349–51.CrossRefGoogle ScholarPubMed
Harnden, D. G. & Brunton, S. (1965). The skin culture technique. In Human Chromosome Methodology. New York: Academic Press.Google Scholar
Marion, G. H., Gier, H. T. & Choudary, J. B. (1968). Micromorphology of the bovine ovarian system. J. Anim. Sci. 27, 451–65.CrossRefGoogle Scholar
Sreenan, J. (1970). In vitro maturation and attempted fertilization of cattle follioular oocytes. J. agric. Sci., Camb. 75, 393–96.CrossRefGoogle Scholar
Suzuki, S. & Mastrolanni, L. (1966). Maturation of monkey ovarian follicular oocytes in vitro. Am. J. Obstet. Gynec. 96, 723–31.CrossRefGoogle ScholarPubMed