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The chemical composition of the pre-sperm fraction of bull ejaculate obtained by electrical stimulation

Published online by Cambridge University Press:  27 March 2009

C. Lutwak-Mann  and
L. B. A. Rowson
C. Lutwak-Mann
Affiliation:
A.R.C. Unit of Animal Reproduction, Huntingdon Road, Cambridge
L. B. A. Rowson
Affiliation:
A.R.C. Unit of Animal Reproduction, Huntingdon Road, Cambridge
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Extract

1. Semen was collected from bulls by means of the electro-ejaculation method developed by Thibault et al. (1948).

2. A copious sperm-free fraction of the ejaculate, produced in the early phase of stimulation, was examined for its chemical composition.

3. This secretion was found to have a low protein content, but its chloride concentration was higher than in the sperm-containing fractions of the ejaculate. Fructose and citric acid occurred in very low concentrations, and 5-nucleotidase was absent in the pre-sperm secretion. This indicates clearly that the secretion does not originate either in the seminal vesicles or in the ampullar glands; it is probable that it represents a discharge from the urethral glands.

4. The effect of the pre-sperm ejaculate fraction upon bull sperm fructolysis and motility was examined; both were unaffected by a considerable excess of this secretion.

5. The chemical composition of the pre-sperm secretion makes it unlikely that under physiological conditions it could play a role in the nutrition of the spermatozoa; presumably, its chief function is the clearing of the urethral passage prior to the descent of the semen proper.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 43 , Issue 2 , April 1953 , pp. 131 - 135
Copyright
Copyright © Cambridge University Press 1953

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References

REFERENCES

Anderson, J. (1945). The Semen of Animals and Its Use for Artificial Insemination. Edinburgh: Imperial Bureau of Animal Breeding and Genetics.Google Scholar
Brown, H. (1945). J. Biol. Chem. 158, 601.CrossRefGoogle Scholar
Day, F. F. (1940). Vet. Rec. 52, 597.Google Scholar
Elson, L. A. & Morgan, W. T. J. (1933). Biochem. J. 27, 1824.CrossRefGoogle Scholar
Fiske, C. H. & Subbarow, Y. (1925). J. Biol. Chem. 66, 375.CrossRefGoogle Scholar
Friedemann, F., Cotonio, M. & Shaffer, P. (1929). J. Biol. Chem. 82, 23.CrossRefGoogle Scholar
Heppel, L. A. & Hilmoe, R. J. (1951). J. Biol. Chem. 188, 665.CrossRefGoogle Scholar
Huggins, C. & Johnson, A. A. (1933). Amer. J. Physiol. 103, 574.CrossRefGoogle Scholar
King, E. J. (1946). Microanalysis in Medical Biochemistry. London: Churchill.Google Scholar
Lambert, W. V. & McKenzie, F. F. (1940). Circ. U.S. Dep. Agric. no. 567.Google Scholar
Lundquist, F. (1949). Acta physiol. Scand. 19, Suppl. 66.Google Scholar
Lutwak-Mann, C. (1951). Biochem. Soc. Symp. 7, 24.Google Scholar
MacLeod, J. & Hotchkiss, R. S.(1942). J. Urol. 48, 225.CrossRefGoogle Scholar
McKenzie, F. F., Miller, J. C. & Bauguess, L. C. (1938). Res. Bull. Mo. agric. Exp. Sta. no. 279.Google Scholar
Mann, T. (1945). Biochem. J. 39, 451.CrossRefGoogle Scholar
Mann, T. (1947). Conference on Infertility, p. 38.Google Scholar
Mann, T. (1948). J. Agric. Sci. 38, 323.CrossRefGoogle Scholar
Mann, T. & Lutwak-Mann, C. (1951). Physiol. Rev. 31, 27.CrossRefGoogle Scholar
Morris, D. L. (1948). Science, 107, 254.CrossRefGoogle Scholar
Phillips, P. H. (1940). J. Amer. Vet. Med. Ass. 97, 165.Google Scholar
Roe, J. H. & Kuether, C. A. (1943). J. Biol. Chem. 147, 399.CrossRefGoogle Scholar
Speck, J. F., Moulder, J. W. & Evans, E. A. (1946). J. Biol. Chem. 104, 119.CrossRefGoogle Scholar
Thibault, C., Laplaud, M. & Ortavant, R. (1948). C.R. Acad. Sci., Paris, 226, 2006.Google Scholar
Whitehorn, J. C. (1921). J. Biol. Chem. 45, 449.CrossRefGoogle Scholar