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Association analysis of SSTR2 copy number variation with cattle stature and its expression analysis in Chinese beef cattle

Published online by Cambridge University Press:  26 September 2019

J. Cheng ,
R. Jiang ,
X. K. Cao ,
M. Liu ,
Y. Z. Huang ,
X. Y. Lan ,
H. Cao ,
C. Z. Lei  and
H. Chen Open the ORCID record for H. Chen [Opens in a new window]
J. Cheng
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China
R. Jiang
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China
X. K. Cao
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China
M. Liu
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China
Y. Z. Huang
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China
X. Y. Lan
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China
H. Cao
Affiliation:
Shaanxi Kingbull Animal Husbandry Co., Ltd., Yangling, Shaanxi 712100, China
C. Z. Lei
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China
H. Chen*
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China
*
Author for correspondence: H. Chen, E-mail: [email protected]
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Abstract

Copy number variations (CNVs), as an important source of genetic variation, can affect a wide range of phenotypes by diverse mechanisms. The somatostatin receptor 2 (SSTR2) gene plays important roles in cell proliferation and apoptosis. Recently, this gene was mapped to a CNV region, which encompasses quantitative trait loci of cattle economic traits including body weight, marbling score, etc. Therefore, SSTR2 CNV may exhibit phenotypic effects on cattle growth traits. In the current study, distribution of SSTR2 gene CNVs was investigated in six Chinese cattle breeds (XN, QC, NY, JA, LX and PN), and the results showed higher CNV polymorphisms in XN, QC and NY cattle. Next, association analysis between growth traits and SSTR2 CNV was performed for XN, QC and NY cattle. In NY, individuals with fewer copies showed better performance than those with more copies. Further, the effects of SSTR2 CNV on the SSTR2 mRNA level were also investigated, but revealed no significant correlation in either muscle or adipose tissue of adult NY cattle. The results suggested the potential for use of SSTR2 CNV as a marker for the molecular breeding of NY cattle.

Keywords

Association analysiscattle staturecopy number variationsmRNA expressionSSTR2
Type
Animal Research Paper
Information
The Journal of Agricultural Science , Volume 157 , Issue 4 , May 2019 , pp. 365 - 374
Copyright
Copyright © Cambridge University Press 2019 

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