Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-24T00:03:33.097Z Has data issue: false hasContentIssue false
  • English
  • Français

Activities of key enzymes involved in starch synthesis in grains of wheat under different irrigation patterns

Published online by Cambridge University Press:  22 April 2009

Z. DAI ,
Y. YIN  and
Z. WANG
Z. DAI
Affiliation:
Biology Department, Dezhou University, Dezhou253023, Shandong, P. R. China Agronomy College, Shandong Agricultural University/National Key Laboratory of Crop Biology/Key Laboratory of Wheat Cultivation Physiology and Genetic Improvement, Ministry of Agriculture, Tai'an271018, Shandong, P. R. China
Y. YIN
Affiliation:
Agronomy College, Shandong Agricultural University/National Key Laboratory of Crop Biology/Key Laboratory of Wheat Cultivation Physiology and Genetic Improvement, Ministry of Agriculture, Tai'an271018, Shandong, P. R. China
Z. WANG*
Affiliation:
Agronomy College, Shandong Agricultural University/National Key Laboratory of Crop Biology/Key Laboratory of Wheat Cultivation Physiology and Genetic Improvement, Ministry of Agriculture, Tai'an271018, Shandong, P. R. China
*
*To whom all correspondence should be addressed. Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

It is generally accepted that sucrose phosphate synthase (SPS), sucrose synthase (SuSy), ADP-glucose pyrophosphorylase (AGPase), soluble starch synthase (SSS), granule-bound starch synthase (GBSS) and starch branching enzyme (SBE) play a key role in starch synthesis in wheat grains. Starch synthesis in wheat grains is influenced by genotype and environment. However, what is not known is the degree of variation in enzyme activities during starch accumulation of wheat cultivars field-grown in different water regimes. The present study was undertaken to determine whether irrigation patterns could cause differences in starch accumulation and activities of key enzymes involved in starch synthesis. Starch accumulation and related enzyme activities were investigated in two winter wheat varieties, JM20 and BY535, differing in grain starch content, under two irrigation patterns. Results showed that soil water deficit led to an increase at early grain filling and decrease during late grain filling in starch accumulation rate (SAR) and activities of key enzymes involved in starch synthesis, especially AGPase, SSS and SBE. Water deficit enhanced grain starch accumulation in two wheat cultivars, suggesting that rainfed treatments increase physiological activities during early grain filling and promote starch accumulation. Furthermore, the change of SAR is consistent with SuSy, AGPase, SSS and GBSS. The results suggest that these enzymes play a key role in starch synthesis, and the decrease of photosynthate produced in the source organ is not the factor inhibiting starch accumulation.

Type
Crops and Soils
Information
The Journal of Agricultural Science , Volume 147 , Issue 4 , August 2009 , pp. 437 - 444
Copyright
Copyright © Cambridge University Press 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Ahmadi, A. & Baker, D. A. (2001). The effect of water stress on the activities of key regulatory enzymes of the sucrose to starch pathway in wheat. Plant Growth Regulation 35, 81–91.Google Scholar
Baroja-Fernández, E., Muñoz, F. J., Saikusa, T., Rodríguez-López, M., Akazawa, T. & Pozueta-Romero, J. (2003). Sucrose synthase catalyzes the de novo production of ADPG linked to starch biosynthesis in heterotrophic tissues of plants. Plant and Cell Physiology 44, 500–509.Google Scholar
Doehlert, D. C., Kuo, T. M. & Felker, F. C. (1988). Enzymes of sucrose and hexose metabolism in developing kernels of two inbreds of maize. Plant Physiology 86, 1013–1019.Google Scholar
Emes, M. J., Bowsher, C. G., Hedley, C., Burrell, M. M., Scrase-Field, E. S. F. & Tetlow, I. J. (2003). Starch synthesis and carbon partitioning in developing endosperm. Journal of Experimental Botany 54, 569–575.CrossRefGoogle ScholarPubMed
Ercoli, L., Lulli, L., Mariotti, M., Masoni, A. & Arduini, I. (2008). Post-anthesis dry matter and nitrogen dynamics in durum wheat as affected by nitrogen supply and soil water availability. European Journal of Agronomy 28, 138–147.CrossRefGoogle Scholar
Fischer, R. A. (2007). Understanding the physiological basis of yield potential in wheat. Journal of Agricultural Science, Cambridge 145, 99–113.Google Scholar
Fisher, D. B. & Gifford, R. M. (1986). Accumulation and conversion of sugars by developing wheat grains. VI. Gradients along the transport pathway from the peduncle to the endosperm cavity during grain filling. Plant Physiology 82, 1024–1030.Google Scholar
Gebbing, T. & Schnyder, H. (1999). Pre-anthesis reserve utilization for protein and carbohydrate synthesis in grains of wheat. Plant Physiology 121, 871–878.CrossRefGoogle ScholarPubMed
Hurkman, W. J., McCue, K. F., Altenbach, S. B., Korn, A., Tanaka, C. K., Kothari, K. M., Johnson, E. L., Bechtel, D. B., Wilson, J. D., Anderson, O. D. & Dupont, F. M. (2003). Effect of temperature on expression of genes encoding enzymes for starch biosynthesis in developing wheat endosperm. Plant Science 164, 873–881.Google Scholar
Jenner, C. F., Ugalde, T. D. & Aspinall, D. (1991). The physiology of starch and protein deposition in the endosperm of wheat. Australian Journal of Plant Physiology 18, 211–226.Google Scholar
Jiang, D., Cao, W. X., Dai, T. B. & Jing, Q. (2003). Activities of key enzymes for starch synthesis in relation to growth of superior and inferior grains on winter wheat (Triticum aestivum) spike. Plant Growth Regulation 41, 247–257.CrossRefGoogle Scholar
Jin, S. B. (1996). Wheat in China. Beijing: China Agricultural Press.Google Scholar
Li, T. G., Shen, B., Chen, N. & Luo, Y. K. (1997). Effect of Q-enzyme on the chalkiness formation of rice grain. Acta Agronomica Sinica 23, 338–344.Google Scholar
Ma, R. K., Jia, X. L., Zhang, Q. G., Zhang, L. H., Yao, Y. R. & Yang, L. H. (2007). Physiological characteristics of water in wheat cultivar SX733: the effect of water-saving irrigation. Acta Agronomica Sinica 33, 1446–1451.Google Scholar
Nakamura, Y., Yuki, K., Park, S. Y. & Ohya, T. (1989). Carbohydrate metabolism in the developing endosperm of rice grains. Plant and Cell Physiology 30, 833–839.Google Scholar
Okita, T. W. (1992). Is there an alternative pathway for starch synthesis? Plant Physiology 100, 560–564.Google Scholar
Singh, S., Singh, G., Singh, P. & Singh, N. (2008). Effect of water stress at different stages of grain development on the characteristics of starch and protein of different wheat varieties. Food Chemistry 108, 130–139.Google Scholar
Wang, W., Zhang, J. H., Yang, J. C. & Zhu, Q. (2004). Effects of controlled soil drought on remobilization of stem stored carbohydrate and grain filling of wheat with unfavorably delayed senescence. Acta Agronomica Sinica 30, 1019–1025.Google Scholar
Wang, Z. L., Yin, Y. P., He, M. R. & Cao, H. M. (1998). Source-sink manipulation effects on postanthesis photosynthesis and grain setting on spike in winter wheat. Photosynthetica 35, 453–459.Google Scholar
Wardlaw, I. F. & Willenbrink, J. (1994). Carbohydrate storage and mobilization by the culm of wheat between heading and grain maturity: The relation to sucrose synthase and sucrose phosphate synthase. Australian Journal of Plant Physiology 21, 255–271.Google Scholar
Xie, Z. J., Jiang, D., Cao, W. X., Dai, T. B. & Jing, Q. (2003). Effects of post-anthesis soil water status on the activities of key regulatory enzymes of starch and protein accumulation in wheat grains. Journal of Plant Physiology and Molecular Biology 29, 309–316.Google Scholar
Xu, Z. Z., Yu, Z. W. & Zhang, Y. L. (2003). The effects of soil moisture on grain starch synthesis and accumulation of winter wheat. Acta Agronomica Sinica 29, 595–600.Google Scholar
Yan, S. H., Wang, Z. L., Dai, Z. M., Li, W. Y., Fu, G. Z., He, M. R. & Yin, Y. P. (2007). Activities of enzymes involved in starch synthesis and accumulation in grains of two wheat cultivars with a different amylose content. Acta Agronomica Sinica 33, 84–89.Google Scholar
Yang, J. C., Zhang, J. H., Wang, Z. Q., Xu, G. W. & Zhu, Q. Z. (2004). Activities of key enzymes in sucrose-to-starch conversion in wheat grains subjected to water deficit during grain filling. Plant Physiology 135, 1621–1629.Google Scholar
Zadocks, J. C., Chang, T. T. & Konzak, C. F. (1974). A decimal code for the growth stages of cereals. Weed Research 14, 415–421.CrossRefGoogle Scholar
Zhang, Y. P., Wang, Z. M., Wang, P. & Zhao, M. (2003). Canopy photosynthetic characteristics of population of winter wheat in water saving and high yielding cultivation. Scientia Agricultura Sinica 36, 1143–1149.Google Scholar