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A simple and rapid method of quantitatively measuring the glucosinolate concentration of rapeseed

Published online by Cambridge University Press:  27 March 2009

D. B. Smith ,
D. G. Parsons  and
Carol Starr
D. B. Smith
Affiliation:
Plant Breeding Institute, Maris Lane, Trumpington, Cambridge, GB2 2LQ
D. G. Parsons
Affiliation:
Plant Breeding Institute, Maris Lane, Trumpington, Cambridge, GB2 2LQ
Carol Starr
Affiliation:
Plant Breeding Institute, Maris Lane, Trumpington, Cambridge, GB2 2LQ
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Summary

A method is described in which glucosinolates are simultaneously extracted from crushed seeds and degraded by myrosinase. Glucose released by myrosinase is measured by an autoanalyser using a single reagent.

Using conditions optimized for samples of 200 mg, yields were slightly greater than those obtained from corresponding samples of defatted meal. Coefficients of variation of about 4% were obtained over a range of more than 100 μmol of glucosinolate/g seed. It is suggested that the method will be of value to plant breeders selecting from populations having low and intermediate concentrations of glucosinolate and to others wishing to obtain a rapid but precise measurement of these important compounds.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 105 , Issue 3 , December 1985 , pp. 597 - 603
Copyright
Copyright © Cambridge University Press 1985

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References

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