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Published online by Cambridge University Press: 27 March 2009
The inoculation of legumes is often carried out using skim-milk as the carrier for the nodule bacteria. Increased germination due to inoculation has often been reported but in this investigation the effects of the skim-milk and the bacteria have been separated. The results indicate that the early establishment of T. ambiguum was stimulated by the skim milk rather than the nodule bacteria. The mechanism of this effect is thought to be due to extra N present in the milk and possibly to an enhanced nitrogen-cycle in the inoculated soil and evidence is produced of increases in certain freeliving nitrogen fixing and nitrifying microorganisms.