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Published online by Cambridge University Press: 27 March 2009
Observations made on freezing tissue show that the appearance of extracellular freezing can occur in tissues which have been initially frozen intracellularly. Since the evidence for the assumption that intracellular freezing is of rare occurrence in the field is based on the microscopical observation of ‘naturally’ frozen tissue it can no longer be assumed that this process is unconnected with the survival of plants overwintering in the field.