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Quantitative digestion of fresh herbage by sheep: IV. Protein synthesis in the stomach

Published online by Cambridge University Press:  27 March 2009

M. J. Ulyatt
Affiliation:
Applied Biochemistry Division, D.S.I.R., Palmerston North, New Zealand
J. C. Macrae
Affiliation:
Applied Biochemistry Division, D.S.I.R., Palmerston North, New Zealand
R. T. J. Clarke
Affiliation:
Applied Biochemistry Division, D.S.I.R., Palmerston North, New Zealand
P. D. Pearce
Affiliation:
Applied Biochemistry Division, D.S.I.R., Palmerston North, New Zealand

Summary

The proportions of bacterial and dietary protein entering the duodenum of sheep fed fresh herbage were assessed using 2,6-diaminopimelic acid as a marker. The herbages fed were Lolium perenne L., ‘Grasslands Ruanui’ perennial ryegrass; L. (perenne × multiflorum), ‘Grasslands Manawa’ short-rotation ryegrass; Trifolium repens L., ‘Grasslands 4700’ white clover.

The dietary protein degraded in the stomach was approximately 70% for all herbages. The bacterial contribution to protein entering the duodenum was 43·1, 57·1 and 52·9% for Ruanui, Manawa and white clover respectively. Protein entering the duodenum contained only a small amount of protozoal protein.

Bacterial protein synthesis in the stomach was different for all three herbages, averaging 16·2, 30·7 and 19·8 g/100 g organic matter apparently digested for Ruanui, Manawa and white clover respectively. It is suggested that the micro-organisms digesting Manawa synthesized protein more efficiently.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1975

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References

REFERENCES

Bailey, R. W. & Jones, D. I. H. (1971). Studies on the hydrolysis by carbohydrases of plant cell-wall constituents in relation to pasture quality. Proceedings of the New Zealand Society of Animal Production 31, 8291.Google Scholar
Brown, G. F., Armstrong, D. G. & MacRae, J. C. (1968). The establishment in one operation of a cannula in the rumen and re-entrant cannulae into the duodenum and the ileum of the sheep. British Veterinary Journal 124, 7882.CrossRefGoogle ScholarPubMed
Clarke, E. M. W., Ellinger, E. M. & Phillipson, A. T. (1966). The influence of diet on the nitrogenous components passing to the duodenum and through the lower ileum of sheep. Proceedings of the Royal Society B 166, 6379.Google Scholar
Clarke, R. T. J. & Hungate, R. E. (1966). Culture of the rumen holotrich ciliate Dasytricha ruminantium Schuberg. Applied Microbiology 14, 340–5.CrossRefGoogle ScholarPubMed
Coehlo da Silva, J. F., Seeley, R. C, Beever, D. E., Prescott, J. H. D. & Armstrong, D. G. (1972 a). The effect in sheep of physical form and stage of growth on the sites of digestion of a dried grass. 2. Sites of nitrogen digestion. British Journal of Nutrition 28, 357–71.CrossRefGoogle Scholar
Coehlo da Silva, J. F., Seeley, R. C, Thomson, D. J., Beever, D. E. & Armstrong, D. G. (1972 b). The effect in sheep of physical form on the sites of digestion of a dried lucerne diet. 2. Sites of nitrogen digestion. British Journal of Nutrition 28, 4361.CrossRefGoogle Scholar
Harrison, D. G., Beever, D. E., Thomson, D. J. & Osbourn, D. F. (1973). The influence of diet upon the quantity and types of amino acids entering and leaving the small intestine of sheep. Journal of Agricultural Science, Cambridge 81, 391401.CrossRefGoogle Scholar
Hogan, J. P. & Weston, R. H. (1967). The digestion of two diets of differing protein content but with similar capacities to sustain wool growth. Australian Journal of Biological Sciences 20, 973–81.Google Scholar
Hogan, J. P. & Weston, R. H. (1970). Quantitative aspects of microbial protein synthesis. In Physiology of Digestion and Metabolism in the Ruminant (ed. Phillipson, A. T.), pp. 474–85. Newcastle upon Tyne: Oriel Press Ltd.Google Scholar
Hume, I. D. (1974). The proportion of dietary protein escaping degradation in the rumen of sheep fed on various protein concentrates. Australian Journal of Agricultural Research 25, 155–65.CrossRefGoogle Scholar
Hungate, R. E., Reichl, J. & Prins, R. (1971). Parameters of rumen fermentation in a continuously fed sheep: evidence of a microbial rumination pool. Applied Microbiology 22, 1104–13.CrossRefGoogle Scholar
Hutton, K., Bailey, F. J. & Annison, E. F. (1971). Measurement of the bacterial nitrogen entering the duodenum of the ruminant using diaminopimelic acid as a marker. British Journal of Nutrition 25, 165–73.CrossRefGoogle ScholarPubMed
Lindsay, J. R. & Hogan, J. P. (1972). Digestion of two legumes and rumen bacterial growth in defaunated sheep. Australian Journal of Agricultural Research 23, 321–30.CrossRefGoogle Scholar
MacRae, J. C. & Ulyatt, M. J. (1974). Quantitative digestion of fresh herbage by sheep. II. The sites of digestion of some nitrogenous constituents. Journal of Agricultural Science, Cambridge 82, 309–19.CrossRefGoogle Scholar
MacRae, J. C, Ulyatt, M. J., Pearce, P. D. & Hendtlass, J. (1972). Quantitative intestinal digestion of nitrogen in sheep given formaldehyde-treated and untreated casein supplements. British Journal of Nutrition 27, 3950.CrossRefGoogle ScholarPubMed
Oxfrod, A. E. (1958). Bloat in cattle. 9. Some observations on the culture of the cattle rumen ciliate Epidinium ecaudatum Crawley occurring in quantity in cows fed on red clover (Trifolium pratense L.). New Zealand Journal of Agricultural Research 1, 809–24.CrossRefGoogle Scholar
Phillipson, A. T. (1964). The digestion and absorption of nitrogenous compounds in the ruminant. In mammalian protein metabolism, vol. I (ed. Munro, H. N. and Allison, J. B.), pp. 71103. New York: Academic Press.CrossRefGoogle Scholar
Pilgrim, A. F., Gray, F. V., Weller, R. A. & Belling, C. B. (1970). Synthesis of microbial protein from ammonia in the sheep's rumen, and the proportion of dietary nitrogen converted into microbial protein. British Journal of Nutrition 24, 589–98.CrossRefGoogle ScholarPubMed
Purser, D. B. & Buechler, S. M. (1966). Amino acid composition of rumen organisms. Journal of Dairy Science 49, 81–4.CrossRefGoogle ScholarPubMed
Ulyatt, M. J. (1969). Progress in denning the differences in nutritive value to sheep of perennial ryegrass, short-rotation ryegrass and white clover. Proceedings of the New Zealand Society of Animal Production 29, 114–23.Google Scholar
Ulyatt, M. J. (1971). Studies on the causes of the differences in pasture quality between perennial ryegrass, short-rotation ryegrass, and white clover. New Zealand Journal of Agricultural Research 14, 352–67.CrossRefGoogle Scholar
Ulyatt, M. J. & MacRae, J. C. (1974). Quantitative digestion of fresh herbage by sheep. I. The sites of digestion of organic matter, energy, readily fermentable carbohydrate, structural carbohydrate, organic acids and lipid. Journal of Agricultural Science, Cambridge 82, 295307.CrossRefGoogle Scholar
Walker, D. J. & Nader, C. J. (1970). Rumen microbial protein synthesis in relation to energy supply: diurnal variation with once-daily feeding. Australian Journal of Agricultural Research 21, 747–54.CrossRefGoogle Scholar
Weller, R. A. (1957). The amino acid composition of the hydrolysates of microbial preparations from the rumen of sheep. Australian Journal of Biological Sciences 10, 384–9.CrossRefGoogle Scholar
Weller, R. A., Gray, F. V. & Pilgrim, A. F. (1958). The conversion of plant nitrogen to microbial nitrogen in the rumen of the sheep. British Journal of Nutrition 12, 421–9.CrossRefGoogle ScholarPubMed
Weller, R. A. & Pilgrim, A. F. (1974). Passage of protozoa and volatile fatty acids from the rumen of the sheep and from a continuous in vitro fermentation system. British Journal of Nutrition 32, 341–51.CrossRefGoogle ScholarPubMed
Williams, C. H., David, D. J. & Iismaa, O. (1962). The determination of chromic oxide in faeces samples by atomic absorption spectrophotometry. Journal of Agricultural Science, Cambridge 59, 381–5.CrossRefGoogle Scholar
Williams, P.P. & Dinusson, W. E. (1973). Amino acid and fatty acid composition of bovine ruminal bacteria and protozoa. Journal of Animal Science 36, 151–5.CrossRefGoogle ScholarPubMed