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Quantitative digestion of fresh herbage by sheep: IV. Protein synthesis in the stomach

Published online by Cambridge University Press:  27 March 2009

M. J. Ulyatt ,
J. C. Macrae ,
R. T. J. Clarke  and
P. D. Pearce
M. J. Ulyatt
Affiliation:
Applied Biochemistry Division, D.S.I.R., Palmerston North, New Zealand
J. C. Macrae
Affiliation:
Applied Biochemistry Division, D.S.I.R., Palmerston North, New Zealand
R. T. J. Clarke
Affiliation:
Applied Biochemistry Division, D.S.I.R., Palmerston North, New Zealand
P. D. Pearce
Affiliation:
Applied Biochemistry Division, D.S.I.R., Palmerston North, New Zealand
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Summary

The proportions of bacterial and dietary protein entering the duodenum of sheep fed fresh herbage were assessed using 2,6-diaminopimelic acid as a marker. The herbages fed were Lolium perenne L., ‘Grasslands Ruanui’ perennial ryegrass; L. (perenne × multiflorum), ‘Grasslands Manawa’ short-rotation ryegrass; Trifolium repens L., ‘Grasslands 4700’ white clover.

The dietary protein degraded in the stomach was approximately 70% for all herbages. The bacterial contribution to protein entering the duodenum was 43·1, 57·1 and 52·9% for Ruanui, Manawa and white clover respectively. Protein entering the duodenum contained only a small amount of protozoal protein.

Bacterial protein synthesis in the stomach was different for all three herbages, averaging 16·2, 30·7 and 19·8 g/100 g organic matter apparently digested for Ruanui, Manawa and white clover respectively. It is suggested that the micro-organisms digesting Manawa synthesized protein more efficiently.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 84 , Issue 3 , June 1975 , pp. 453 - 458
Copyright
Copyright © Cambridge University Press 1975

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