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Piperine as a new natural supplement with beneficial effects on the life-span and defence system of honeybees

Published online by Cambridge University Press:  24 June 2019

M. Schulz ,
A. Łoś ,
M. Grzybek ,
R. Ścibior  and
A. Strachecka Open the ORCID record for A. Strachecka [Opens in a new window]
M. Schulz
Affiliation:
Institute of Biological Basis of Animal Production, Faculty of Biology, Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland
A. Łoś
Affiliation:
Institute of Biological Basis of Animal Production, Faculty of Biology, Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland Institute of Nature Conservation, Polish Academy of Sciences, 31-120 Cracow, Poland
M. Grzybek
Affiliation:
Department of Zoology, Animal Ecology & Wildlife Management, Faculty of Biology, Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland Department of Tropical Parasitology, Medical University of Gdansk, 81-519 Gdynia, Poland
R. Ścibior
Affiliation:
Department of Zoology, Animal Ecology & Wildlife Management, Faculty of Biology, Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland
A. Strachecka*
Affiliation:
Institute of Biological Basis of Animal Production, Faculty of Biology, Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland
*
Author for correspondence: A. Strachecka, E-mail: [email protected]
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Abstract

Many factors, including pathogens, environmental change and breeding techniques, affect honeybee immunity/resistance, so substances and natural supplements that enhance it are desired. To eliminate the impact of unknown external factors, in 2016 a cage experiment was conducted under constant laboratory conditions (35 °C, 65% relative humidity). Bees in the control group were fed with sugar dissolved in water at ratio 1:1 ad libitum with no additives, while the other group was fed with sugar syrup (1:1) supplemented with piperine (3 µg/ml) ad libitum. The piperine-treated workers lived 9 days longer compared to the control group. In the piperine-consuming group, protein concentration and the activities of antioxidative enzymes, such as superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione S-transferase (GST), were higher than in the control group. The activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were also higher in the piperine-treated group. Neutral and acidic proteases inhibitors, as well as neutral protease activities, were higher in the haemolymph of the piperine-treated workers than in untreated bees. Acidic protease activities in the haemolymph were higher in untreated workers only on days 18 and 32. Alkaline protease activities in the control bees were higher from day 10. From 10 days old, the total antioxidant capacity level was significantly higher in the haemolymph of piperine-treated workers. Piperine decreased DNA methylation levels significantly in the older bees. The compound could have the potential to be a natural diet supplement increasing apian resistance to stress factors.

Keywords

Apis melliferabiochemical parametersdefence systemnatural supplementationpiperine
Type
Crops and Soils Research Paper
Information
The Journal of Agricultural Science , Volume 157 , Issue 2 , March 2019 , pp. 140 - 149
Copyright
Copyright © Cambridge University Press 2019 

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