Hostname: page-component-78c5997874-ndw9j Total loading time: 0 Render date: 2024-11-03T05:24:49.970Z Has data issue: false hasContentIssue false

A method for estimating the number of active protozoa in the soil

Published online by Cambridge University Press:  27 March 2009

D. W. Cutler
Affiliation:
(Rothamsted Experimental Station.)

Extract

1. A method is described by which it is possible to estimate the numbers of active protozoa in a soil.

2. The total number of protozoa is first found by a dilution method. A fresh portion of the soil is then treated with 2 per cent. HCl (sp. gr. 1·15) overnight. By this means all active forms are killed. A second count by the dilution method gives the number of cystic protozoa in the soil. From these results the number of active forms can be ascertained.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1920

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

(1)Barratt, J. O. W. (1905). “Die Kohlensäure-produktion von Paramoecium aureliaZeitschr. f. allg. Physiologie, Bd. 5.Google Scholar
(2)Cropper, J. W., and Drew, A. H. (1914). “Researches into Induced Cell-Reproduction in Amoebae.” McFadden Researches, 4.Google Scholar
(3)Cunningham, A. (1915). “Studies on Soil Protozoa.” Journ. Agric. Science, 7, 49.CrossRefGoogle Scholar
(4)Cutler, D. W. (1919). “Observations on Soil Protozoa.” Ib. 9, 430.Google Scholar
(5)Goodey, T. (1913). “The Excystation of Colpoda cucullus from its resting cysts and the nature and properties of the cyst membrane.” Proc. Roy. Soc., B, 86, 427.Google Scholar
(6)Itano, A. and Ray, G. B. (1918). “Method for counting certain Protozoa in the Soil.” Soil Science, 5, 303.Google Scholar
(7)Koch, G. P. (1916). “Studies on Soil Protozoa.” Ib. 2, 163.Google Scholar
(8)Kopeloff, N., Lint, H. C., and Coleman, D. A. (1916). “A New Method for counting Soil Protozoa.” Cent. f. Bakt. II. Bd. 45, 230.Google Scholar
(9)Martin, C. H., and Lewin, K. R. (1915). “Notes on some methods for the examination of Soil Protozoa.” Journ. Agric. Science, 7, 106.CrossRefGoogle Scholar
(10)Wenyon, C. M., and O'Connor, F. W. (1917). “An inquiry into some problems affecting the spread and incidence of intestinal protozoal infections, etc.Journ. Roy. Army Med. Corps, 28, 151.Google Scholar