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Longevity of ram sperm in various diluents and at different dilution rates
Published online by Cambridge University Press: 27 March 2009
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The effect of various diluents and different dilution rates on the maintenance of motility of ram sperm during storage at 4° C. was tested. Their efficiency was determined by half-life periods and total survival times of the sperm.
A yolk-glycine-citrate diluent maintained sperm motility for a longer period than did yolk—fructose—phosphate, yolk-citrate, cow's milk and ewe's milk, with or without yolk. This diluent has the advantage that the sperm is clearly visible under the microscope, and no sedimentation of any component occurs.
A modified IVT diluent prolonged the total life span of the sperm, but caused a faster motility decline in the first part of the storage period.
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