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Influence Of Aspergillus oryzae fermentation extract on the fermentation of a basal ration in the rumen simulation technique (Rusitec)

Published online by Cambridge University Press:  27 March 2009

P. P. Frumholtz ,
C. J. Newbold  and
R. J. Wallace
P. P. Frumholtz
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB, UK
C. J. Newbold
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB, UK
R. J. Wallace
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB, UK
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Summary

The effects of adding a culture extract from Aspergillus oryzae (AO) to the fermentation of a basal ration were investigated using the rumen simulation technique (Rusitec). The ration consisted of hay, barley, molasses, fishmeal and a minerals/vitamins mixture, at 500, 299–5, 100, 91 and 9–5 g/kg dry matter respectively. AO eliminated the transient fall in pH that occurred following the addition of substrate to Rusitec, but had no influence on the buffering capacity of the medium. Increases in the acetate: propionate ratio and in the proportion of butyrate in the fermentation products were observed in vessels receiving AO. Ammonia concentrations increased by over 30%. The number of total viable bacteria almost doubled and the cellulolytic population increased three-fold in vessels receiving AO. Protozoal numbers were reduced by almost 45% by AO. The proportion of methane in the headspace gas collected from the vessels during the day was significantly (P < 0·01) lower in vessels receiving AO. These results demonstrate that AO has a marked effect on the microbial population and the stoichiometry of the rumen fermentation, and that these changes can be seen in an in vitro system.

Type
Review
Information
The Journal of Agricultural Science , Volume 113 , Issue 2 , October 1989 , pp. 169 - 172
Copyright
Copyright © Cambridge University Press 1989

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References

REFERENCES

Arambel, M. J., Weidmeier, R. D. & Walters, J. L. (1987). Influence of donor animal adaptation to added yeast culture and/or Aspergillus oryzae fermentation extract on in vitro rumen fermentation. Nutrition Reports International 35, 433–436.Google Scholar
Boing, J. T. P. (1983). Enzyme Production. In Industrial Microbiology (4th edn; (Ed. Reed, G.), pp.685–689. Westport, CT, USA: AVI Publishing Co., Inc.Google Scholar
Bryant, M. P. (1972) Commentary on the Hungate technique for culture of anaerobic bacteria. Americal Journal of Clinical Nutrition 25, 1324–1328.CrossRefGoogle ScholarPubMed
Czerkawski, J. W.&Breckenridge, G. (1977). Design and development of a long-term rumen simulation technique (Rusitec). British Journal of Nutrition 38, 371–384.CrossRefGoogle ScholarPubMed
Goodall, S. R. & Byers, F. M. (1978). Automated micromethod for enzymatic L( + ) and D(−) lactic acid determinations in biological fluid containing cellular extracts. Analytical Biochemistry 89, 80–86.CrossRefGoogle Scholar
Harris, N., Van Horn, H. H., Manookian, K. E., Marshall, S. P., Taylor, M. J. & Wilcox, C. J. (1983). Sugar cane silage, sodium hydroxide and steam pressure treated sugar cane bagasse, corn silage, cottonseed hulls, sodium bicarbonate and Aspergillus oryzae product in complete rationsfor lactating cows. Journal of Dairy Science 66, 1474–1485.CrossRefGoogle Scholar
Hillman, K. (1987). Studies on metabolism in rumen protozoa. PhD thesis, University of Wales, University College, Cardiff, UK.Google Scholar
Hobson, P. N. (1969). Rumen bacteria. In Methods in Microbiology 3B, 133–149. Academic Press.Google Scholar
Mann, S. O. (1968). An improved method for determining cellulolytic activity in anaerobic bacteria. Journal of Applied Bacteriology 31, 241–244.CrossRefGoogle Scholar
McDougall, E. O. (1948). Studies on ruminant saliva. 1. The composition and output of sheep's saliva. Biochemical Journal 43, 99–109.CrossRefGoogle ScholarPubMed
Newbold, C. J., Williams, A. G. & Chamberlain, D. G. (1987). The in vitro metabolism of D, L-lactic acid by rumen microorganisms. Journal of the Science of Food and Agriculture 38, 9–19.CrossRefGoogle Scholar
Snedecor, G. N. & Cochran, G. C. (1976). Statistical Methods. Ames, Iowa, USA: Iowa State University Press.Google Scholar
Stewart, C. S. (1977). Factors affecting the cellulolytic activity of rumen contents. Applied and Environmental Microbiology 33, 497–502.CrossRefGoogle ScholarPubMed
Stewart, C. S. &Duncan, S. H. (1985). The effect of avoparcin on cellulolytic bacteria of the ovine rumen. Journal of General Microbiology 131, 427–435.Google Scholar
Stewart, C. S., Paniagua, C., Dinsdale, D., Cheng, K. -J. & Garrow, S. H. (1981). Selective isolation and characteristics of Bacteroides succinogenes from the rumen of a cow. Applied and Environmental Microbiology 41, 504–510.CrossRefGoogle Scholar
Van Horn, H. H., Harris, B., Taylor, M. J., Bachman, K. C. & Wilcox, C. J. (1984). By-product feeds for lactating dairy cows: effects of cottonseed hulls, sunflower hulls, corrugated paper, peanut hulls, sugarcane bagasse, and whole cottonseed with additives of fat, sodium bicarbonate and Aspergillus oryzae product on milk production. Journal of Dairy Science 67, 2922–2938.CrossRefGoogle Scholar
Whitehead, R., Cooke, G. H. & Chapman, B. T. (1967). Problems associated with the continuous monitoring of ammonical nitrogen in river water. Automation in Analytical Chemistry 2, 377–380.Google Scholar
Weidmeier, R. D., Arambel, M. J.&Walters, J. L. (1987). Effect of yeast culture and Aspergillus oryzae fermentation extract on ruminal characteristics and nutrient digestibility. Journal of Dairy Science 70, 2063–2068.CrossRefGoogle Scholar
Williams, P. E. V. (1989). Understanding the biochemical mode of action of yeast culture. In Biotechnology in the Feed Industry. Proceedings of Alltech's 4th Annual Symposium (Ed.Lyons, T. P.), pp.79–99. USA: Alltech Technical Publications.Google Scholar