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Influence of Aspergillus oryzae fermentation extract on rumen fermentation and blood constituents in sheep given diets of grass hay and barley

Published online by Cambridge University Press:  27 March 2009

C. J. Newbold ,
P. P. Frumholtz  and
R. J. Wallace
C. J. Newbold
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB, UK
P. P. Frumholtz
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB, UK
R. J. Wallace
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB, UK
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Summary

Aspergillus oryzae fermentation extract (AO; 2 g/day) was added to the diet of sheep fed grass hay supplemented with 30 or 70% barley. AO decreased the proportion of propionate in rumen volatile fatty acids with both levels of supplementation (146 and 163 mmol/mol with AO v. 157 and 186 mmol/mol with no addition for the low and high barley diets respectively; P < 0·05), and also caused a small reduction in L-lactate concentration in the high barley diet (1·15 mM v. 1·43 mM in the absence of AO). Rumen pH was not changed significantly. Total viable bacteria in the rumen were stimulated with AO (2·12 and 2·46 v. 0·97 and 1·80 × 109/ml respectively). Numbers of cellulolytic bacteria and ciliate protozoa were unchanged. Hay suspended in nylon bags in the rumen tended to be degraded more rapidly with AO, but the effect was not statistically significant. Neither barley nor AO significantly altered the plasma concentrations of glucose, urea, insulin, gastrin or cholecystokinin. It was concluded that the effects of AO were generally similar for both levels of barley supplementation and that changing the composition of the diet had no detectable effect on the measured indicators of nutritional status in blood.

Type
Animals
Information
The Journal of Agricultural Science , Volume 119 , Issue 3 , December 1992 , pp. 423 - 427
Copyright
Copyright © Cambridge University Press 1992

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References

REFERENCES

Arambel, M. J., Wiedmeier, R. D. & Walters, J. L. (1987). Influence of donor animal adaptation to added yeast culture and/or Aspergillus oryzae fermentation extract on in vitro rumen fermentation. Nutrition Reports International 35, 433–436.Google Scholar
Bassett, J. M. & Thorburn, G. D. (1971). The regulation of insulin secretion by the ovine foetus in utero. Journal of Endocrinology 50, 59–74.CrossRefGoogle ScholarPubMed
Blair, E. L., Grund, E. R., Reed, J. D., Sanders, D. J., Sanger, G. & Shaw, B. (1975). The effect of sympathetic nerve stimulation on serum gastrin, gastric acid secretion and mucosal blood flow responses to meat extract stimulation in anaesthetized cats. Journal of Physiology 253, 493–504.CrossRefGoogle ScholarPubMed
Chademana, I. & Offer, N. W. (1990). The effect of dietary inclusion of yeast culture on digestion in sheep. Animal Production 50, 483–489.Google Scholar
Davidson, J., Mathieson, J. & Boyne, A. W. (1970). The use of automation in determining nitrogen by the Kjeldahl method, with final calculations by computer. Analyst 95, 181–193.CrossRefGoogle ScholarPubMed
Dawson, K. A. (1987). Mode of action of the yeast culture, Yea-sacc, in the rumen: a natural fermentation modifier. In Biotechnology in the Feed Industry: Proceedings of Alltech's 2nd Annual Symposium (Ed. Lyons, T. T.), pp. 119–126. Nicholasville, KY: Alltech Technical Publications.Google Scholar
Dockray, G. J. (1980). Cholecystokinins in rat cerebral cortex: identification, purification and characterization by immunochemical methods. Brain Research 188, 155–165.CrossRefGoogle ScholarPubMed
Fondevila, M., Newbold, C. J., Hotten, P. M. & Ørskov, E. R. (1990). A note on the effect of Aspergillus oryzae fermentation extract on the rumen fermentation of sheep given straw. Animal Production 51, 422–425.Google Scholar
Firkins, J. L., Weiss, W. P., Eastridge, M. L. & Hull, B. L. (1990). Effects of feeding fungal culture extract and animal-vegetable fat on degradation of hemicellulose and on ruminal bacterial growth in heifers. Journal of Dairy Science 73, 1812–1822.CrossRefGoogle ScholarPubMed
Frumholtz, P. P. (1991). Manipulation of the rumen fermentation and its effects on digestive physiology. PhD thesis, University of Aberdeen.Google Scholar
Frumholtz, P. P., Newbold, C. J. & Wallace, R. J. (1989). Influence of Aspergillus oryzae fermentation extract on the fermentation of a basal ration in the rumen simulation technique (Rusitec). Journal of Agricultural Science, Cambridge 113, 169–172.CrossRefGoogle Scholar
Genstat 5 Committee (1987). Genstat 5 Users’ Manual, Oxford: Oxford University Press.Google Scholar
Goering, H. K. & Van, Soest P. J. (1970). Forage Fibre Analysis. Agricultural Handbook, US Department of Agriculture, No 379.Google Scholar
Gomez-Alarcon, R. A., Dudas, C. & Huber, J. T. (1990). Influence of cultures of Aspergillus oryzae on rumen and total tract digestibility of dietary components. Journal of Dairy Science 73, 703–710.CrossRefGoogle ScholarPubMed
Gomez-Alarcon, R. A., Huber, J. T., Higginbotham, G. E., Wiersma, F., Ammon, D. & Taylor, B. (1991). Influence of feeding Aspergillus oryzae fermentation extract on the milk yields, eating patterns, and body temperature of lactating cows. Journal of Animal Science 69, 1733–1740.CrossRefGoogle ScholarPubMed
Huber, J. T., Higginbotham, G. E. & Ware, D. R. (1985). Influence of feeding vitaferm, containing an enzyme-producing culture from Aspergillus oryzae, on performance of lactating cows. Journal of Dairy Science 68, 122 (Abstract).Google Scholar
Itabashi, H., Kobayashi, T. & Matsumoto, M. (1983). Effect of absence of ciliate protozoa on concentration of plasma free amino acids, insulin and gastrin in ruminants. In Proceedings of the Fifth World Conference on Animal Production, Volume 2, pp. 339–340. Tokyo: Japanese Society of Zootechnical Science.Google Scholar
Itabashi, H., Kobayashi, T., Matsumoto, M. & Takenaka, A. (1989). Effect of unfaunation on plasma free amino acids, gastrin and insulin, and duodenal nitrogen flow in ruminants. Asian-Australasian Journal of Animal Sciences 2, 489–490.CrossRefGoogle Scholar
Kellems, R. O., Lagerstedt, A. & Wallentine, M. V. (1990). Effect of feeding Aspergillus oryzae fermentation extract or Aspergillus oryzae plus yeast culture plus mineral and vitamin supplement on performance of Holstein cows during a complete lactation. Journal of Dairy Science 73, 2922–2928.CrossRefGoogle ScholarPubMed
Mann, S. O. (1968). An improved method for determining cellulolytic activity in anaerobic bacteria. Journal of Applied Bacteriology 31, 241–244.CrossRefGoogle Scholar
Marsh, W. H., Fingerhut, B. & Miller, H. (1965). Automated and manual methods for determination of blood urea. Clinical Chemistry 11, 624–627.CrossRefGoogle ScholarPubMed
Mehrez, A. Z. & Ørskov, E. R. (1977). A study of the artificial fibre bag technique for determining the digestibility of feeds in the rumen. Journal of Agricultural Science, Cambridge 88, 645–650.CrossRefGoogle Scholar
Newbold, C. J., Brock, R. & Wallace, R. J. (1991). Influence of autoclaved or irradiated Aspergillus oryzae fermentation extract on fermentation in the rumen simulation technique (Rusitec). Journal of Agricultural Science, Cambridge 116, 159–162.CrossRefGoogle Scholar
Nisbet, D. J. & Martin, S. A. (1990). Effect of dicarboxylic acids and Aspergillus oryzae fermentation extract on lactate uptake by the ruminal bacterium Selenomonas ruminantium. Applied and Environmental Microbiology 56, 3515–3518.CrossRefGoogle ScholarPubMed
Perry, K. W. (1986). Factors effecting the release and activity ofgaslrin in ruminants. PhD thesis, University of Newcastle upon Tyne.Google Scholar
Reynolds, G. W., Simpson, H. V., Carr, D. H. & McLeay, L. M. (1991). Gastrin: its molecular forms and secretion in sheep. In Physiological Aspects of Digestion and Metabolism in Ruminants (Eds T., Tsuda, Y., Sasaki & R., Kawashima), pp. 63–87. Tokyo: Academic Press.CrossRefGoogle Scholar
Trinder, P. (1969). Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Annals of Clinical Biochemistry 6, 24—27.CrossRefGoogle Scholar
Wiedmeier, R. D. (1989). Optimizing the utilization of low quality forages through supplementation and chemical treatment. In 9th Annual Utah Beef Cattle Field Day. Provo, Utah: Brigham Young University.Google Scholar
Wiedmeier, R. D., Arambel, M. J. & Walters, J. L. (1987). Effect of yeast culture and Aspergillus oryzae fermentation extract on ruminal characteristics and nutrient digestibility. Journal of Dairy Science 70, 2063–2068.CrossRefGoogle ScholarPubMed
Williams, P. E. V., Tait, C. A. G., Innes, G. M. & Newbold, C. J. (1991). Effects of the inclusion of yeast culture (Saccharomyces cerevisiae plus growth medium) in the diet of dairy cows on milk yield and forage degradation and fermentation patterns in the rumen of steers. Journal of Animal Science 69, 3016–3026.CrossRefGoogle ScholarPubMed