Hostname: page-component-cd9895bd7-8ctnn Total loading time: 0 Render date: 2024-12-26T06:17:50.407Z Has data issue: false hasContentIssue false
  • English
  • Français

In vitro maturation and attempted fertilization of cattle follicular oocytes

Published online by Cambridge University Press:  27 March 2009

J. Sreenan
J. Sreenan
Affiliation:
Faculty of Agriculture, University College Dublin
Get access Rights & Permissions [Opens in a new window]

Summary

Maturation studies were carried out on 241 cattle oocytes. Media used were bovine follicular fluid and growth medium. Both media supported a resumption of meiosis in ca. 80 % of cases, but growth medium proved superior in terms of final maturation.

Fertilization attempts were carried out on 564 oocytes following culture (27–32 h) in growth medium. In vitro fertilization attempts (107 oocytes) and in vivo fertilization attempts (82 oocytes) in the rabbit yielded negative results. Following in vivo fertilization attempts in the ewe, ova containing pronuclei as well as apparently normal cleaved ova were recovered.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 75 , Issue 3 , December 1970 , pp. 393 - 396
Copyright
Copyright © Cambridge University Press 1970

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Austin, C. R. (1961). Manipulation of eggs. In The Mammalian Egg. Oxford: Blackwell Scientific publications.CrossRefGoogle Scholar
Brock, H. & Rowson, L. E. A. (1952). The production of viable bovine ova. J. agric. Sci., Gamb. 42, 479–82.CrossRefGoogle Scholar
Chang, M. C. (1955). The maturation of rabbit oocytes in culture and their maturation, activation, fertilization and subsequent development in the Fallopian tubes. J. exp. Zool. 123, 379–99.CrossRefGoogle Scholar
Dziuk, P. J., Donker, J. D., Nichols, J. R. & Petersen, W. E. (1958). Problems associated with the transfer of ova between cattle. Tech. Bull. Minn, agric. Exp. Stn no. 222.Google Scholar
Edwards, R. G. (1962). Meiosis in ovarian ooeytes of adult mammals. Nature, Lond. 196, 446–50.CrossRefGoogle Scholar
Ewards, R. G. (1965). Maturation in vitro of mouse, sheep, cow, pig, rhesus monkey and human ovarian oocytes. Nature, Lond. 208, 349–51.CrossRefGoogle Scholar
Edwards, R. G., Bavister, B. D. & Steptoe, P. C. (1969). Early stages of fertilization in vitro of human oocytes matured in vitro. Nature, Lond. 221, 631–5.CrossRefGoogle ScholarPubMed
Hafez, E. S. E., Stjoie, T. & Gordon, I. (1963). Superovulation and related phenomena in the beef cow. I. Superovulatory responses following PMS and HCG injections. J. Reprod. Fert. 5, 59–79.CrossRefGoogle ScholarPubMed
Hamner, C. E. & Sojka, N. T. (1967). Capacitation of rabbit spermatazoa; species specificity and organ specificity. Proc. Soc. exp. Biol. Med. 124, 689–91.CrossRefGoogle Scholar
Harnden, D. G. & Brunton, S. (1965). The skin culture technique. In Human chromosome methodology. New York: Academic Press.Google Scholar
Henricson, B. & Rajakoski, E. (1959). Studies of oocytogensis in cattle. Cornell Vet. 49, 494–503.Google ScholarPubMed
Kirton, K. T., Boyd, L. J. & Hafs, H. D. (1968). Fertility of bull semen with added amylase. Proc. VI Congr. Int. Reprod. Anim. Insem. Artif. Parie 1, 59–61.Google Scholar
Marion, G. B., Gier, H. T. & Chotjdary, J. B. (1968). Micromorphology of the bovine ovarian follicular system. J. Anim. Sci. 27, 451–71.CrossRefGoogle ScholarPubMed
Quirke, J. F. (1969). Culture and attempted fertilization of the sheep follicular oocyte. M. Agr. Sc. Thesis, University College, Dublin.Google Scholar
Rowson, L. E. A. (1951). Methods of inducing multiple ovulations in cattle. J. Endocr. 7, 260–70.CrossRefGoogle ScholarPubMed
Scaklon, P. (1970). Studies in reproduction in the cow. Ph.D. Thesis, University College, Dublin.Google Scholar
Sreenan, J., Scanlon, P. & Gordon, I. (1968). Culture of fertilized cattle eggs. J. agric. Sci., Camb. 70, 183–5.CrossRefGoogle Scholar
Suztjki, S. & Mastroianni, L. (1968). The fortilizability of in vitro cultured monkey ovarian follicular oocytes. Fert. Steril. 19, 500–8.Google Scholar