Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-03T05:01:34.918Z Has data issue: false hasContentIssue false

Genetic diversity in perennial ryegrass (Lolium perenne) evaluated by hybridization with ribosomal DNA: implications for cultivar identification and breeding

Published online by Cambridge University Press:  01 August 1998

K. M. F. WARPEHA
Affiliation:
Department of Applied Plant Science, Queen's University of Belfast, Belfast, UK
I. CAPESIUS
Affiliation:
Botanisches Institut, Universität Heidelberg, D-69120 Heidelberg, Germany
T. J. GILLILAND
Affiliation:
Department of Applied Plant Science, Queen's University of Belfast, Belfast, UK Department of Agriculture for Northern Ireland, Plant Testing Station, Crossnacreevy, Belfast, UK

Abstract

To date, little molecular genetic research has been conducted in Lolium and the current study is the first investigation of diversity in ribosomal DNA (rDNA) among perennial ryegrass cultivars. Ribosomal DNA probes derived from the intergenic spacer (IGS–M) and transcribed (COD–M) regions of mustard (Sinapis alba) were used to examine 35 perennial ryegrass (L. perenne L.) cultivars for genetic diversity using RFLP methodology. Analysis of digested genomic DNA on Southern blots revealed only 10 cultivars with unique RFLP patterns. Genetic diversity was exclusively associated with the IGS–M probe, as the cultivars were all indistinguishable when examined with the COD–M probe. This low incidence of distinctions was not expected, given that the 35 cultivars were known genetically distinct entities, representing the very wide range of the major phenotypic and physiological characteristics present in commercial stocks. Furthermore, the forage cultivars were proportionately less polymorphic in comparison to the amenity cultivars. This was also unexpected as amenity cultivars are recognized as a more closely clustered group than the forage cultivars, based on morphological characteristics. The potential use of rDNA for assessing cultivar diversity and breeding histories in perennial ryegrass is considered.

Type
Research Article
Copyright
© 1998 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)