Effect of the addition of cell wall degrading enzymes on fermentation kinetics of perennial ryegrass silage

Abstract

Two studies were undertaken to evaluate the effects of cellulase and endoxylanase enzymes on the chemical composition and the fermentation characteristics of grass silage, using the gas production technique. Perennial ryegrass was ensiled in 1 litre glass containers for 90 days with cellulase (0·2 g/kg grass on fresh weight basis) and endoxylanase in the concentrations of 0·05 g/kg grass (Endox 0·05) and 0·2 g/kg grass (Endox 0·2). Subsequently, dried samples, ground through a 1 mm screen, were used in chemical analysis and gas production measurements. In Expt 1, the enzyme treatment significantly decreased silage NDF (P < 0·001), ADF (P < 0·001) and acetic acid (P < 0·01) concentrations and increased lactic acid (P < 0·001) production. In Expt 2, lower concentrations of NDF (P < 0·001) and ADF (P < 0·001) in treated silages resulted in increased sugar concentration (P < 0·001). In this experiment, butyric acid was detected. Addition of cellulase and Endox 0·05 enzymes did not alter silage digestibility. In both studies, cellulase and Endox 0·2 treatments tended to increase the rate of gas production within 10 hours of inoculation with rumen fluid whereas Endox 0·05 had no effect. The volume of gas produced was however greater for the untreated silage than for the enzyme-treated silage samples after 48 h of incubation. The strategy of applying cellulase and endoxylanase to the herbage in the ensiling process proved to be effective in modifying the chemical composition and increasing sugar concentration and the rate of gas production of the silages. Further research on the factors determining enzyme effectiveness is therefore suggested to elucidate the mechanisms leading to higher utilization of the released sugars.