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The effect of inclusion of mineral salts in the diet on dilution rate, the pattern of rumen fermentation and the composition of the rumen microflora

Published online by Cambridge University Press:  27 March 2009

D. J. Thomson ,
D. E. Beever ,
M. J. Latham ,
M. E. Sharpe  and
R. A. Terry
D. J. Thomson
Affiliation:
The Grassland Research Institute, Hurley, Maidenhead, Berks SL6 5LR
D. E. Beever
Affiliation:
The Grassland Research Institute, Hurley, Maidenhead, Berks SL6 5LR
M. J. Latham
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RQ2 9AT
M. E. Sharpe
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RQ2 9AT
R. A. Terry
Affiliation:
The Grassland Research Institute, Hurley, Maidenhead, Berks SL6 5LR
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Summary

The mixed buffer salts of artificial saliva were incorporated at three rates: 0 (diet 1), 5·7 (diet 2) and 11·4% (diet 3) (w/w) in pelleted diets prepared from60% ground flaked maize and 40% dried ground grass. The control and mineral-supplemented diets were fed to sheep hourly (Expt 1) or twice daily (Expt 2).

When the sheep were fed hourly or twice daily the pattern of fermentation in the rumen was altered from a predominantly propionate to mainly acetate fermentation. Dilution rate in the rumen increased from 0·036 to 0·053 to 0·064 when diets 1, 2 and 3 were fed hourly, and dilution rate was negatively related to the molar proportion of propionic acid (P < 0·05) and positively related (P < 0·05) to the molar proportion of acetic acid in rumen fluid, but these relationships were not apparent in Expt 2.

Nitrogen retention was higher for lambs fed diet 2 than for those fed diets 1 and 3 (P < 0·05) in Expt 1.

In sheep fed hourly, selenomonads and bacteroides were the predominant rumen bacteria associated with the control diet but the inclusion of mineral salts resulted in the bacteroides being replaced by large number of chain-forming cocci. Numbers of protozoa were variable. The relationship of these changes to the fermentation pattern is discussed. In sheep fed twice daily there was much variation between animals.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 91 , Issue 1 , August 1978 , pp. 1 - 7
Copyright
Copyright © Cambridge University Press 1978

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References

Attar, A. Al., Evans, R. A. & Axfobd, R. F. E. (1976). The effect of frequency of feeding and of the dietary energy source upon microbial protein synthesis in the rumen of sheep. Proceedings of the Nutrition Society 35, 108 A.Google Scholar
Beever, D. E., Thomson, D. J. & Cammell, S. B. (1976). The digestion of frozen and dried forage by sheep. Journal of Agricultural Science, Cambridge 86, 443–452.Google Scholar
Blaxter, K. L. (1967). The Energy Metabolism of Ruminants, 2nd ed.London: Hutchinson.Google Scholar
Bryant, M. P. & Robinson, I. M. (1961). An improved non-selective culture medium for ruminal bacteria and its use in determining diurnal variation in numbers of bacteria in the rumen. Journal of Dairy Science 44, 1446–1456.Google Scholar
Downes, A. M., Reis, P. J., Shabby, L. E. & Tunes, D. A. (1970). Evaluation of modified [358]methionine and [35s]casein preparations as supplements for sheep. British Journal of Nutrition 24, 1083–1089.CrossRefGoogle ScholarPubMed
Feigl, F. (1960). In Spot Tests in Organic Chemistry. London: Elsevier.Google Scholar
Harrison, D. G. (1974). A simple method for the determination of rumen dilution rate in sheep. Newsletter on the Application of Nuclear Methods to Biology and Agriculture, no. 3, 8–9.Google Scholar
Harrison, D. G., Beever, D. E. & Thomson, D. J. (1974).The effect of dilution rate upon fermentation in the rumen. Proceedings of the Nutrition Society 33, 43A.Google Scholar
Harrison, D. G., Beever, D. E., Thomson, D. J. & Osbourn, D. F. (1975). Manipulation of rumen fermentation in sheep by increasing the rate of flow of water from the rumen. Journal of Agricultural Science, Cambridge 85, 93–101.CrossRefGoogle Scholar
Harrison, D. G., Beever, D. E., Thomson, D. J. & Osbourn, D. F. (1976). Manipulation of fermentation in the rumen. Journal of the Science of Food and Agriculture 27, 617–620.CrossRefGoogle ScholarPubMed
Hodgson, J. C. & Thomas, P. C. (1975). A relationship between the molar proportion of propionic acid and the clearance rate of the liquid phase in the rumen of sheep. British Journal of Nutrition 33, 447–456.CrossRefGoogle Scholar
Hungate, R. E. (1969). In Methods in Microbiology, vol. 38. London and New York: Academic Press.Google Scholar
Hungate, R. E., Reichl, J. & Prins, R. (1974). Parameters of rumen fermentation in a continuously fed sheep; evidence of a microbial rumination pool. Applied Microbiology 22, 1104–1113.CrossRefGoogle Scholar
Kellaway, R. C., Thomson, D. J. & Beeveb, D. E. (1975). The effect of sodium chloride and sodium bicarbonate on food intake, growth rate and acidbase balance in calves. Proceedings of the Nutrition Society 35, 63 A.Google Scholar
Latham, M. J. & Sharpe, M. E. (1975). Rumen microbial population of lambs given mineral supplementeddiets. Proceedings of the Nutrition Society 34, 113 A.Google Scholar
Latham, M. J., Sutton, J. D. & Shabpe, M. E. (1974). Fermentation and micro-organisms in the rumen and the content of fat in the milk of cows given low roughage rations. Journal of Dairy Science 57, 803–810.Google Scholar
McDougall, E. I. (1948). Studies on ruminant saliva. Biochemical Journal 43, 99–109.Google Scholar
Minson, D. J. (1966). Diurnal variation in the excretion of faeces and urine by sheep fed once daily or at hourly intervals. British Journal of Nutrition 20, 757–764.Google Scholar
Scheifinger, C. C. & Wolin, M. J. (1973). Propionate formation of cellulose and soluble sugars by combined cultures of Bacteroides succinogenes and Selenomonas ruminantium. Applied Microbiology 27, 789–795.Google Scholar
Snedecor, G. W. (1956). Statistical Methods, 5th ed.Ames, Iowa: Iowa State, College Press.Google Scholar
Thomson, D. J., Beever, D. E., Mundell, D. C., Elderiteld, M. L. & Hakrison, D. G. (1975). The effect of altering dilution rate on the pattern of fermentation in the rumen. Proceedings of the Nutrition Society 34, 111 A.Google Scholar
Warner, A. C. I. & Stacy, B. D. (1968). The fate of water in the rumen. British Journal of Nutrition 22, 369–387.Google Scholar
Weller, R. A., Gray, F. V., Pilgrim, A. F. & Jones, G. B. (1967). The rates of production of volatile fatty acids in the rumen. Australian Journal of Agricultural Research 18, 107–118.CrossRefGoogle Scholar