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The effect of changes in the intestinal flow of nucleic acids on allantoin excretion in the urine of Sheep

Published online by Cambridge University Press:  27 March 2009

Anna M. Antoniewicz
Affiliation:
Department of Animal Nutrition, Institute of Animal Production, Sarego 2, Krakow, Poland
W. W. Heinemann
Affiliation:
Irrigated Agriculture Research and Extension Center, Washington State University, Prosser, WA 99350, U.S.A.
E. M. Hanks
Affiliation:
Irrigated Agriculture Research and Extension Center, Washington State University, Prosser, WA 99350, U.S.A.

Summary

Three ewes were intraduodenally infused with yeast RNA (15, 20 and 30 g/day) or control solutions for 3 days and the net changes in urinary allantoin excretion determined. Four mature wethers fitted with a re-entrant cannula in the proximal duodenum were fed two levels (0·66 and 1 × maintenance) of pelleted lucerne hay. Allantoin excretion was compared with total nucleic acid (NA) flow in the small intestine.

The average proportion of the recovery of the infused RNA-N dose as urinary allantoin–N amounted to 0·119 ± 0·0046. The values of the net increase of allantoin-N (Y, g/day) above control levels were highly significantly correlated (n = 12, r = 0·98) with amounts of RNA-N infused (X, g/day): y = 0·1185 (± 0·0086) X–0·004 (P < 0·001). The average ratio of urinary allantoin-N to NA-N passing the duodenum during 24 h was 0·173. A difference between the two conversion factors is discussed in relation to endogenous allantoin excretion and purine base composition of yeast and bacterial RNA.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1980

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