Hostname: page-component-78c5997874-ndw9j Total loading time: 0 Render date: 2024-11-03T05:36:50.165Z Has data issue: false hasContentIssue false
  • English
  • Français

Culture and fertilization of sheep ovarian oocytes:III. Evidence on fertilization in the sheep oviduct based on pronucleate and cleaved eggs

Published online by Cambridge University Press:  27 March 2009

J. F. Quirke  and
I. Gordon
J. F. Quirke
Affiliation:
Faculty of Agriculture, University College, Dublin
I. Gordon
Affiliation:
Faculty of Agriculture, University College, Dublin
Get access Rights & Permissions [Opens in a new window]

Summary

Four hundred and fifteen oocytes cultured in growth medium for 24·5–28·5 h were transferred to the oviducts of 23 oestrous ewes. Twenty ewes were bred prior to transfer; three remained unbred as a control. After intervals of 24–73 h oocytes were recovered. In 179 oocytes from 19 bred recipients, 19 pronucleateand 26 cleaved eggs were recovered; one apparent 4-cell egg was found in 37 oocytes recovered from unbred ewes.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 76 , Issue 3 , June 1971 , pp. 375 - 377
Copyright
Copyright © Cambridge University Press 1971

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Crosby, T. F. & Gordon, I., (1971). Culture and fertilisation of sheep ovarian oocytes. II. Timing of nuclear maturation in oocytes cultured in growth medium. J. agric. Sci., Camb. 76, 373–4.CrossRefGoogle Scholar
Dziuk, P. J. (1965). Timing of maturation and fertilization of the sheep egg. Anat. Rec. 153, 211–23.CrossRefGoogle ScholarPubMed
Lamond, D. R. & Urquhart, D. (1961). Sheep laparotomy cradle. Aust. vet. J. p. 430–433.CrossRefGoogle Scholar
Quirke, J. F. & Gordon, I. (1971). Culture and fertilisation of sheep ovarian oocytes. I. Effect of culture medium on resumption of meiosis. J. agric. Sci., Camb. 76, 369–72.CrossRefGoogle Scholar