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The cellulose-lignin complex in forages and its relationship to forage nutritive value

Published online by Cambridge University Press:  27 March 2009

D. W. Allinson  and
D. F. Osbourn
D. W. Allinson
Affiliation:
The Grassland Research Institute, Hurley, Berkshire
D. F. Osbourn
Affiliation:
The Grassland Research Institute, Hurley, Berkshire
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Summary

The relationships between the cellulose-lignin complex, voluntary consumption and dry-matter digestibility were examined using forages of two varieties of Italian ryegrass and two legumes, lucerne and sainfoin. These forages had previously been shown to exhibit different intake-digestibility relationships. The cellulose, acid detergent lignin and acid detergent fibre contents of these forages as well as their digestibility coefficients were determined. Lignins were extracted from the fibre fractions and their ultraviolet difference spectra determined.

Changes in maturity of a forage during a single growth phase, produced changes in dry-matter digestibility which were closely associated with changes in the digestibility of the cellulose and inversely related to the lignin content of the forage. Differences in digestibility between varieties of forage were less closely associated with lignin content. As the grasses matured a lignin fraction forming a difference peak at 350 mμ became evident and this was associated with decreased cellulose digestibility.

Differences in voluntary food consumption resulting from changes in maturity of a single forage variety, in one growth phase, were also closely correlated with both dry matter and cellulose digestibility and inversely with lignin content. Differences between varieties and growth phases of the ryegrasses and between grasses and legumes were related less to the nature of the cellulose-lignin complex and more closely to the percentage of the total digesta deriving from cellulose.

Sainfoin was characterized by the formation post-ruminally of an artifact that analysed quantitatively as lignin and qualitatively resembled a non-conjugated phenolic lignin fraction. The production of this artifact apparently disturbed fibre digestibility estimates and may have affected dry-matter digestibility estimates. Degradation of lignin apparently occurred both in the rumen and in the hind gut.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 74 , Issue 1 , February 1970 , pp. 23 - 36
Copyright
Copyright © Cambridge University Press 1970

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References

REFERENCES

Aulin-Erdtman, G. (1955). The application of δE curves to chemical problems. Chemy Ind. 20, 581–2.Google Scholar
Balch, C. C. & Campling, R. C. (1962). Regulation of voluntary intake in ruminants. Nutr. Abstr. Rev. 32, 669–86.Google ScholarPubMed
Balch, D. A., Balch, C. C. & Rowland, J. J. (1954). The influence of the method of determination of lignin on the lignin-ratio technique for digestibility in the cow. J. Sci. Fd Agric. 5, 584–8.CrossRefGoogle Scholar
Baumgardt, B. R. & Ho., Hi Kon (1964). Evaluation of forages in the laboratory. IV. Within and among trial variability of the Wisconsin artificial rumen procedure. J. Dairy Sci. 47, 263–6.CrossRefGoogle Scholar
Bondi, A. H. & Meyer, H. (1943 a). Lignins in young plants. Biochem. J. 43, 248–56.CrossRefGoogle Scholar
Bondi, A. H. & Meyer, H. (1943 b). On the chemical nature and digestibility of roughage carbohydrates. J. agric. Sci., Camb. 33, 123–8.CrossRefGoogle Scholar
Colburn, M. W. & Evans, J. L. (1967). Chemical composition of the cell-wall constituent and acid detergent fiber fractions of forages. J. Dairy Sci. 50, 1130–5.CrossRefGoogle Scholar
Dehority, B. A. (1961). Effect of particle size on the digestion rate of purified cellulose by rumen cellulolytic bacteria in vitro. J. Dairy Sci. 44, 687–92.CrossRefGoogle Scholar
Dehority, B. A., Johnson, R. R. & Conrad, H. R. (1962). Digestibility of forage hemicellulose and pectin by rumen bacteria in vitro and the effects of lignification thereon. J. Dairy Sci. 45, 508–12.CrossRefGoogle Scholar
Demarquilly, C., Boissau, J. M. & Cuylle, G. (1966). Factors affecting the voluntary intake of green forage by sheep. Proc. 9th Int. Grassld Congr. Brazil877–85.Google Scholar
Donefer, E., Cramtpon, E. W. & Lloyd, L. E. (1966). The prediction of digestible energy intake potential (N.V.I.) of forages using a simple in vitro technique. Xth Int. Grassld Congr., Helsinki 442–5.Google Scholar
Ely, R. E., Kane, E. A., Jacobson, W. C. & Moore, L. A. (1953). Studies on the composition of lignin isolated from orchardgrass hay cut at four stages of maturity and from the corresponding faeces. J. Dairy Sci. 36, 346–55.CrossRefGoogle Scholar
Ghose, S. N. & King, G. W. (1963). The effects of physical and chemical properties of cellulosic fibers on anaerobic deterioration by pure cultures. Textile Res. J. 33, 392–8.CrossRefGoogle Scholar
Goldschmid, O. (1954). Determination of phenolic hydroxyl content of lignin preparations by ultraviolet spectrometry. Analyt. Chem. 26, 142–3.CrossRefGoogle Scholar
Halliwell, G. & Bryant, M. P. (1963). The cellulolytic activity of pure strains of bacteria from the rumen of cattle. J. gen. Microbiol. 32, 441–8.CrossRefGoogle ScholarPubMed
Osbourn, D. F. (1967). The intake of Conserved Forages. Contained in Fodder Conservation. Edit. Wilkins, R. J.. Occas. Symp. no. 3 Br. Grassld Soc., pp. 20–8.Google Scholar
Osbourn, D. F., Thomson, D. J. & Terry, R. A. (1966). The relationship between voluntary intake and digestibility of forage crops, using sheep. Proc. Xth Int. Grassld Congr., Helsinki, pp. 363–7.Google Scholar
Stafford, H. A. (1960 a). Differences between ligninlike polymers formed by peroxidation of eugenol and ferulic acid in leaf sections of Phleum. Pl. Physiol. 35, 108–14.CrossRefGoogle ScholarPubMed
Stafford, H. A. (1960 b). Comparison of lignin-like polymers produced peroxidatively by cinnamic acid derivatives in leaf sections of Phleum. Pl. Physiol. 35, 612–18.CrossRefGoogle ScholarPubMed
Stafford, H. A. (1962). Histochemical and biochemical differences between lignin-like materials in Phleum pratense L. Pl. Physiol. 37, 643–9.CrossRefGoogle ScholarPubMed
Stafford, H. A. (1964). Comparison of lignin-like products found naturally or induced in tissues of Phleum, Elodia, and Coleus, and in a paper peroxidase system. Pl. Physiol. 39, 350–60.CrossRefGoogle ScholarPubMed
Sullivan, J. T. (1955). Cellulose and lignin in forage grasses and their digestion coefficients. J. Anim. Sci. 14, 710–7.CrossRefGoogle Scholar
Sullivan, J. T. (1962). Methods for the analysis of forage plants with particular reference to carbohydrate constituents. U.S.D.A. Dept. G.R.—81–62.Google Scholar
Tilley, J. M. A. & Terry, R. A. (1963). A two-stage technique for the in vitro digestion of forage crops. J. Br. Grassld Soc. 18, 104–11.CrossRefGoogle Scholar
Van Soest, P. J. (1963). Use of detergents in the analysis of fibrous feeds. II. A rapid method for the determination of fibre and lignin. J. Ass. off. agric. Chem. 46, 928–35.Google Scholar
Van Soest, P. J. (1964). Symposium on nutrition and forages and pasture: new chemical procedures for evaluating forages. J. Anim. Sci. 23, 838–45.CrossRefGoogle Scholar
Van Soest, P. J. (1965). Symposium on factors influencing the voluntary intake of herbage by ruminants: voluntary intake in relation to chemical composition and digestibility. J. Anim. Sci., 24, 834–43.CrossRefGoogle Scholar
Van Soest, P. J. (1966). Non-nutritive residues: A system of analysis for the replacement of crude fibre. J. ass. off. agric. Chem. 49, 546–51.Google Scholar
Van Soest, P. J. (1967). Development of a comprehensive system of feed analysis and its application to forages. J. Anim. Sci. 26, 119–28.CrossRefGoogle Scholar
Wexler, A. J. (1964). Characterization of lignosulfonates by ultravoilet spectrometry; direct and difference spectrograms. Analyt. Chem. 36, 213–21.CrossRefGoogle Scholar