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An evaluation of the use of dihaploids and unreduced gametes in breeding for quantitative resistance to potato pathogens

Published online by Cambridge University Press:  27 March 2009

M. J. De Maine
M. J. De Maine
Affiliation:
Scottish Crop Research InstitutePentlandfield, Roslin, Midlothian, Scotland
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Summary

In a breeding programme to combine quantitative resistance to two important potato pathogens, Phytophthora infestans causing late blight in foliage and Qlobodera pallida (the cream potato cyst-nematode), tetraploid (4 ×) off spring were obtained by crossing dihaploids, mainly as females, with tetraploids. Glasshouse and field tests in the first and second tuber-years showed that most of the off spring of highly blight-resistant dihaploids had high resistance to P. infestans. Two such dihaploids, PDH 182 and PDH 247, crossed with a tetraploid selected for high general combining ability for potato cyst-nematode (PCN) resistance, gave off spring all of which were highly blight resistant. About one third also had high PCN resistance.

Detached leaflet tests were used for rapid blight resistance screening of other progenies from dihaploid × 4 × crosses in their seedling year. Differences were found in the effects of 4 × parents on the mean resistances of progenies from blight-resistant dihaploids.

The results also showed differences between dihaploids in their effectiveness in transmitting blight resistance to 4 × offspring. This could be due to differences in the mode of unreduced female gamete formation, i.e. first division restitution or second division restitution (SDR). If all the unreduced gametes were produced by SDR, the differences could be due to differences in homozygosity between dihaploids with respect to blight resistance genes.

The dihaploid × 4 × crosses gave few offspring per pollination. The few seedlings obtained may be offset by a higher frequency with the required characters. Since fewer seedlings would have to be grown in order to find the required combination of characters, savings could be made in planting and resistance-testing facilities over those required in conventional breeding.

The tetraploid hybrids obtained can be used in a second cycle of breeding in three ways simultaneously: by crossing with other tetraploids, by crossing with dihaploids selected for complementary characters and to produce dihaploids with combined resistances.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 99 , Issue 1 , August 1982 , pp. 79 - 83
Copyright
Copyright © Cambridge University Press 1982

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References

REFERENCES

De Maine, M. J. (1978 a). Field resistance to late blight and potato root eel worm in Group Tuberosum diliaploids. Euphytica 27, 305–315.CrossRefGoogle Scholar
De Maine, M. J. (1978 b). A new approach to the use of dihaploids in breeding for increased pathogen resistance in potatoes. 7th Triennial Conference of the European Association for Potato Research, Warsaw, p. 171.Google Scholar
Kidane-Mariam, H.-M. & Peloquin, S. J. (1971). Effect of direction of hybridisation (4 × × 2 × or 2 × × 4 ×) on yield in cultivated potatoes. American Potato Journal 48, 301.Google Scholar
Malcolmson, J. F. (1969). Factors involved in resistance to blight (Phytophthora infestans Mont de Bary) in potatoes and assessment of resistance using detached leaves. Annals of Applied Biology 64, 461–468.CrossRefGoogle Scholar
McLean, J. G. & Stevensen, F. J. (1952). Methods of obtaining seed on Russet Burbank and similar flowering varieties of potatoes. American Potato Journal 29, 206–211.CrossRefGoogle Scholar
Mendiburu, A. O. & Peloquin, S. J. (1977). The significance of 2n gametes in potato breeding. Theoretical and Applied Genetics 49, 53–61.CrossRefGoogle ScholarPubMed
Mendiburu, A. O., Peloquin, S. J. & Mok, D. W. S. (1974). Potato breeding with haploids and 2n gametes. In Haploids in Higher Plants (ed. Kasha, K. J.), pp. 249–258. University of Guelph, Guelph.Google Scholar
Mok, D. W. S. & Peloquin, S. J. (1975). The inheritance of three mechanisms of diplandroid (2n pollen) formation in diploid potatoes. Heredity 35, 295—302.CrossRefGoogle Scholar
Phillips, M., Forrest, J. M. S. & Wilson, L. A. (1980). Screening for resistance to potato cyst nematode using closed containers. Annals of Applied Biology 96, 317–322.CrossRefGoogle Scholar
Taylor, L. M. (1978). Variation patterns of parthenogenetic plants derived from unreduced embryo sacs of Solatium tuberosum subspecies andigena (Juz. et Buk) Hawkes. Theoretical and Applied Genetics 52, 241–249.CrossRefGoogle Scholar