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Amylolytic activity and chemical composition of rehydrated ground maize ensiled with α-amylase or glucoamylase

Published online by Cambridge University Press:  02 October 2019

J. R. Gandra ,
E. R. Oliveira ,
C. S. Takiya ,
T. A. Del Valle Open the ORCID record for T. A. Del Valle [Opens in a new window] ,
F. P. Rennó ,
R. H. T. B. Goes Open the ORCID record for R. H. T. B. Goes [Opens in a new window] ,
R. S. R. Leite ,
N. F. L. Garcia ,
J. D. O. Batista  and
A. P. Acosta
...Show all authors
J. R. Gandra*
Affiliation:
Department of Animal Sciences, School of Agrarian Sciences, Federal University of Grande Dourados, Dourados, Brazil
E. R. Oliveira
Affiliation:
Department of Animal Sciences, School of Agrarian Sciences, Federal University of Grande Dourados, Dourados, Brazil
C. S. Takiya
Affiliation:
Department of Animal Sciences and Industry, Kansas State University, Manhattan, USA
T. A. Del Valle
Affiliation:
Campus Itaqui, Federal University of Pampa, Itaqui, Brazil
F. P. Rennó
Affiliation:
Department of Animal Nutrition and Production, University of Sao Paulo, Pirassununga, Brazil
R. H. T. B. Goes
Affiliation:
Department of Animal Sciences, School of Agrarian Sciences, Federal University of Grande Dourados, Dourados, Brazil
R. S. R. Leite
Affiliation:
Department of Biological and Environmental Sciences, Federal University of Grande Dourados, Dourados, Brazil
N. F. L. Garcia
Affiliation:
Department of Animal Nutrition and Production, University of Sao Paulo, Pirassununga, Brazil
J. D. O. Batista
Affiliation:
Department of Animal Sciences, School of Agrarian Sciences, Federal University of Grande Dourados, Dourados, Brazil
A. P. Acosta
Affiliation:
Department of Animal Sciences, School of Agrarian Sciences, Federal University of Grande Dourados, Dourados, Brazil
J. Damiani
Affiliation:
Department of Animal Sciences, School of Agrarian Sciences, Federal University of Grande Dourados, Dourados, Brazil
E. R. S. Gandra
Affiliation:
Department of Animal Sciences, Institute for the Study of the Humid Tropics, Federal University of Southern and Southeastern Pará, Xinguara, Brazil
A. Z. Escobar
Affiliation:
Department of Animal Sciences, School of Agrarian Sciences, Federal University of Grande Dourados, Dourados, Brazil
*
Author for correspondence: J. R. Gandra, E-mail: [email protected]
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Abstract

A completely randomized experiment was designed to evaluate the effects of α-amylase (AMY) and glucoamylase (GLU) on total losses, fermentative profile, chemical composition and amylolytic activity of rehydrated maize. Eighty-four experimental silos of rehydrated maize [0.33 litres/kg ground maize, 4-mm theoretical particle size, and 625 g/kg dry matter (DM)] were assigned to the following treatments: (1) control (CON), no enzyme addition; (2) GLU added at 300 µl/kg of ground maize (as-fed); and (3) AMY added at 300 µl/kg of ground maize. Seven silos from each treatment were opened after 7, 14, 21 and 28 days. Differences among treatments were evaluated through orthogonal contrasts (CON v. enzymes, and AMY v. GLU). Time effects were decomposed using polynomial regression. Glucoamylase silage exhibited greater total losses than AMY. Enzymes increased acetate and lactic acid concentrations and decreased ethanol concentration. Regardless of treatment, gas, effluent and total fermentative losses linearly increased, whereas DM recovery linearly decreased with higher storage length. Glucoamylase silage had lower ammonia nitrogen and higher lactic acid concentrations than AMY. Enzyme treatments decreased silage neutral detergent fibre content and increased in vitro DM degradation. Glucoamylase silage exhibited a more moderate starch content and greater in vitro DM degradation than AMY. Storage time linearly decreased DM, starch and fibre content of rehydrated maize. In vitro degradation of DM linearly increased as the storage length increased. This study showed evidence that enzymes with amylolytic activity, particularly GLU, improve the fermentative profile and DM degradation of rehydrated maize silage.

Keywords

Enzymeskernels silagereducing sugarsilage fermentationstarch
Type
Animal Research Paper
Information
The Journal of Agricultural Science , Volume 157 , Issue 5 , July 2019 , pp. 449 - 455
Copyright
Copyright © Cambridge University Press 2019 

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References

Andreazzi, SR, Pereira, MN, Reis, RB, Pereira, RAN, Morais Júnior, NN, Acedo, TS, Hermes, RG and Cortinhas, CS (2018) Effect of exogenous amylase on lactation performance of dairy cows fed a high-starch diet. Journal of Dairy Science 101, 7199–7207.Google Scholar
AOAC (Association of Official Analytical Chemists) (2000) Official Methods of Analysis, 7th Edn. Arlington, VA, USA: AOAC.Google Scholar
Bach Knudsen, KE (1997) Carbohydrate and lignin contents of plant materials used in animal feeding. Animal Feed Science and Technology 64, 319–338.Google Scholar
Carvalho, BF, Ávila, CL, Bernardes, TF, Pereira, MN, Santos, C and Schwan, RF (2017) Fermentation profile and identification of lactic acid bacteria and yeasts of rehydrated corn kernel silage. Journal of Applied Microbiology 122, 589–600.Google Scholar
Chen, L, Guo, G, Yu, CQ, Zhang, J, Shimojo, M and Shao, T (2015) The effects of replacement of whole-plant corn with oat and common vetch on the fermentation quality, chemical composition and aerobic stability of total mixed ration silage in Tibet. Animal Science Journal 86, 69–76.Google Scholar
Correa, CES, Shaver, RD, Pereira, MN, Lauer, JG and Kohn, K (2002) Relationship between corn vitreousness and ruminal in situ starch degradability. Journal of Dairy Science 85, 3008–3012.Google Scholar
Da Silva, TC, Smith, ML, Barnard, AM and Kung, L Jr (2015) The effect of a chemical additive on the fermentation and aerobic stability of high-moisture corn. Journal of Dairy Science 98, 8904–8912.Google Scholar
da Silva, NC, Nascimento, CF, Nascimento, FA, de Resende, FD, Daniel, JLP and Siqueira, GR (2018) Fermentation and aerobic stability of rehydrated corn grain silage treated with different doses of Lactobacillus buchneri or a combination of Lactobacillus plantarum and Pediococcus acidilactici. Journal of Dairy Science 101, 4158–4167.Google Scholar
Ding, MY, Koizumi, H and Suzuki, Y (1995) Comparison of three chromatographic systems for determination of organic acids in wine. Analytical Sciences 11, 239–243.Google Scholar
Fernandes, T, Silva, KT, Gomide, DR, Pereira, RAN, Avilas, CLS and Pereira, NM (2016) Effect of glucoamylase and duration of silage storage on ruminal degradation and dry matter loss of corn and sorghum grain rehydrated and ensiled (abstract). Journal of Dairy Science 99(E-1), 673.Google Scholar
Ferraretto, LF, Taysom, K, Taysom, DM, Shaver, RD and Hoffman, PC (2014) Relationships between dry matter content, ensiling, ammonia-nitrogen, and ruminal in vitro starch digestibility in high-moisture corn samples. Journal of Dairy Science 97, 3221–3227.Google Scholar
Ferraretto, LF, Fredin, SM and Shaver, RD (2015) Influence of ensiling, exogenous protease addition, and bacterial inoculation on fermentation profile, nitrogen fractions, and ruminal in vitro starch digestibility in rehydrated and high-moisture corn. Journal of Dairy Science 98, 7318–7327.Google Scholar
Foldager, J (1977) Protein Requirement and Non-Protein Nitrogen for High Producing Cow in Early Lactation (PhD Diss). Michigan State University, East Lasing, MI, USA.Google Scholar
Hmidet, N, Bayoudh, A, Berrin, JG, Kanoun, S, Juge, N and Nasri, M (2008) Purification and biochemical characterization of a novel α-amylase from Bacillus licheniformis NH1: Cloning, nucleotide sequence and expression of amyN gene in Escherichia coli. Process Biochemistry 43, 499–510.Google Scholar
Hoffman, PC, Esser, NM, Shaver, RD, Coblentz, WK, Scott, MP, Bodnar, AL, Schmidt, RJ and Charley, RC (2011) Influence of ensiling time and inoculation on alteration of the starch-protein matrix in high-moisture corn. Journal of Dairy Science 94, 2465–2474.Google Scholar
Holden, LA (1999) Comparison of methods of in vitro dry matter digestibility for ten feeds. Journal of Dairy Science 82, 1791–1794.Google Scholar
Jafari-Aghdam, J, Khajek, K, Ranjbar, B and Nemat-Gorgani, M (2005) Deglycosylation of glucoamylase from Aspergillus niger: effects on structure, activity and stability. Biochimica et Biophysica Acta 1750, 61–68.Google Scholar
Jensen, C, Weisbjerg, MR, Nørgaard, P and Hvelplund, T (2005) Effect of maize silage maturity on site of starch and NDF digestion in lactating dairy cows. Animal Feed Science and Technology 118, 279–294.Google Scholar
Karim, KMR, Husaini, A, Hossain, MA, Sing, NN, Sinang, FM, Hussain, MHM and Roslan, HA (2016) Heterologous, expression, and characterization of thermostable glucoamylase derived from Aspergillus flavus NSH9 in Pichia pastoris. BioMed Research International 2016, 112–119.Google Scholar
Kenward, MG and Roger, JH (1997) Small sample inference for fixed effects from restricted maximum likelihood. Biometrics 53, 983–997.Google Scholar
Lara, EC, Bragiato, UC, Rabelo, CHS, Messana, JD and Reis, RA (2018) Inoculation of corn silage with Lactobacillus plantarum and Bacillus subtilis associated with amylolytic enzyme supply at feeding. 1. Feed intake, apparent digestibility, and microbial protein synthesis in wethers. Animal Feed Science and Technology 243, 22–34.Google Scholar
Lawton, JW (2002) Zein: a history of processing and use. Cereal Chemistry 79, 1–18.Google Scholar
Leahy, KT, Barth, KM, Hunter, PP and Nicklas-Bray, SA (1990) Effects of treating corn silage with alpha-amylase and(or) sorbic acid on beef cattle growth and carcass characteristics. Journal of Animal Science 68, 490–497.Google Scholar
Lopes, NM, Cardoso, PC and Pereira, MN (2016) Effect of glucoamylase, particle size, and duration of silage storage on dry matter loss and digestibility of ground corn rehydrated and ensiled (abstract). Journal of Animal Science 94(suppl. 5), 671, 10.2527/jam2016-1388.Google Scholar
McDonald, P, Henderson, AR and Heron, SJE (1991) The Biochemistry of Silage, 2nd Edn. Marlow, UK: Chalcombe Publications.Google Scholar
Miller, GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31, 426–428.Google Scholar
Nozière, P, Steinberg, W, Silberberg, M and Morgavi, DP (2014) Amylase addition increases starch ruminal digestion in first-lactation cows fed high and low starch diets. Journal of Dairy Science 97, 2319–2328.Google Scholar
Pandey, A (1995) Glucoamylase research: an overview. Starch 47, 439–445.Google Scholar
Pérez, S, Baldwin, PM and Gallant, DJ (2009) Structural features of starch granules. In BeMiller, J and Whistler, R (eds), Starch: Chemistry and Technology, 3rd Edn. New York, USA: Academic Press, pp. 149–192.Google Scholar
Queiroz, OCM, Ogunade, IM, Weinberg, Z and Adesogan, AT (2018) Silage review: foodborne pathogens in silage and their mitigation by silage additives. Journal of Dairy Science 101, 4132–4142.Google Scholar
Rodrigues, PHM, Gomes, RCG, Meyer, PM, Borgatti, LMO, Franco, FMJ and Godoy, GLA (2012) Effects of microbial inoculants and amino acid production by-product on fermentation and chemical composition of sugarcane silages. Revista Brasileira de Zootecnia 41, 1394–1400.Google Scholar
Santos, MC, Lock, AL, Mechor, GD and Kung, L Jr (2015) Effects of a spoilage yeast from silage on in vitro ruminal fermentation. Journal of Dairy Science 98, 2603–2610.Google Scholar
Sutton, JD, Morant, SV, Bines, JA, Napper, DJ and Givens, DI (1993) Effect of altering the starch: fibre ratio in the concentrates on hay intake and milk production by Friesian cows. Journal of Agricultural Science, Cambridge 120, 379–390.Google Scholar
Takiya, CS, Calomeni, GD, Silva, TH, Vendramini, THA, Silva, GG, Consentini, CEC, Bertoni, JC, Zilio, EMC and Rennó, FP (2017) Increasing dietary doses of an Aspergillus oryzae extract with alpha-amylase activity on nutrient digestibility and ruminal fermentation of lactating dairy cows. Animal Feed Science and Technology 228, 159–167.Google Scholar
Tricarico, JM, Johnston, JD, Dawson, KA, Hanson, KC, McLeod, KR and Harmon, DL (2005) The effects of an Aspergillus oryzae extract containing alpha-amylase activity on ruminal fermentation and milk production in lactating Holstein cows. Animal Science 81, 365–374.Google Scholar
Van Soest, PJ, Robertson, JB and Lewis, BA (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.Google Scholar
Vargas-Rodriguez, CF, Engstrom, M, Azem, E and Bradford, BJ (2014) Effects of dietary amylase and sucrose on productivity of cows fed low-starch diets. Journal of Dairy Science 97, 4464–4470.Google Scholar
Weiss, WP, Steinberg, W and Engstrom, MA (2011) Milk production and nutrient digestibility by dairy cows when fed exogenous amylase with coarsely ground dry corn. Journal of Dairy Science 94, 2492–2499.Google Scholar
Wong, DWS, Robertson, GH, Lee, CC and Wagschal, K (2007) Synergistic action of recombinant α-amylase and glucoamylase on the hydrolysis of starch granules. The Protein Journal 26, 159–164.Google Scholar