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Variation in Lipophorin Titres During Development in Solitarious and Gregarious Schistocerca gregaria (Forskål) (Orthoptera: Acrididae)

Published online by Cambridge University Press:  19 September 2011

D. O. Ogoyi  and
E. O. Osir
D. O. Ogoyi
Affiliation:
Department of Biochemistry, University of Nairobi, P. O. Box 30197, Nairobi, Kenya
E. O. Osir
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi, Kenya
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Abstract

The levels of lipophorin, the principal insect haemolymph lipoprotein, were estimated during the development of solitary and gregarious phases of Schistocerca gregaria using single radial immunodiffusion. In the gregarious phase, lipophorin titres ranged from 6.69 ± 0.57 mg/ml in the 3rd nymphal instar to 14.42 ± 2.04 mg/ml in mature adults. The titres in the solitary phase were 3.33 ± 0.59 mg/ml in the 3rd nymphal instar and 8.44 ± 0.67 mg/ml in mature adults. Peak lipophorin titres occurred in mature adults (14.42 ± 2.04 mg/ml) and 5th nymphal instar (8.75 ± 0.25 mg/ml) for gregarious and solitary locusts, respectively. Gregarious locusts had significantly (P< 0.05) higher lipophorin titres than their solitary counterparts during the 3rd and 4th nymphal instars as well as in the adult stage (P< 0.01). Estimation of the haemolymph protein levels over the same period showed a general increase from the 3rd nymphal instar to mature adult stage in both phases. It is proposed that the higher lipophorin titre in gregarious locusts is a physiological adaptation that ensures high lipid reserves that are necessary to cope with the high metabolic requirements of this phase.

Résumé

Les taux de lipophorine, la principale lipoprotéine de l'hémolymphe des insectes, ont été estimés pendant le développement des phases solitaires et grégaires de Schistocerca gregaria à l'aide de l'immunodiffusion radiale simple. Chez la phase grégaire, les teneurs en lipophorine sont comprises entre 6.69 ±0.57 mg/ml pour le 3éme stade juvénile et 14.42 ± 2.04 mg/ml pour les adultes matures. Chez la phase solitaire, les teneurs sont de 3.33 ± 0.59 mg/ml pour le 3ème stade juvénile et de 8.44 ± 0.67mg/ml pour les adultes matures. Les teneurs les plus élevées en lipophorine sont rencontrées sur les adultes matures (14.42 ± 2.04 mg/ml) et le 5éme stade juvénile (8.75 ± 0.25 mg/ml) pour les criquets grégaires et solitaires, respectivement.

Les criquets grégaires ont des teneurs significativement plus élevées (P < 0.05) que leurs homologues solitaires pendant les stades juvéniles 3 et 4, de même que chez le stade adulte (P < 0.01). Pendant la même période, pour les deux phases, l'estimation des teneurs en protéines de l'hémolymphe montre un accroissement général lorsque l'on passe du 3éme stade juvénile au stade adulte. Les plus fortes teneurs en lipophorine chez les criquets grégaires seraient une adaptation physiologique leur permettant de disposer d'importantes réserves lipidiques nécessaires à leurs fortes exigences métaboliques.

Keywords

Schistocerca gregariaphase statusdevelopmentlipophorinfat bodylipid reservesadipokinetic hormoneSchistocerca gregariaphasedéveloppementlipophorinecorps grasréserves lipidiqueshormone adipocinétique
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 21 , Issue 1 , March 2001 , pp. 73 - 79
Copyright
Copyright © ICIPE 2001

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