Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-30T20:21:58.888Z Has data issue: false hasContentIssue false
  • English
  • Français

Spore replication rate of Nosema bombycis (Microsporidia: Nosematidae) in the silkworm Bombyx mori L. in relation to pupal development and age of moths

Published online by Cambridge University Press:  19 September 2011

T. O. Sasidharan ,
R. N. Singh ,
M. V. Samson ,
A. Manjula ,
P. C. Santha  and
Chandrashekharaiah
T. O. Sasidharan*
Affiliation:
Silkworm Seed Technology Laboratory, Carmelram Post, Kodathi, Bangalore, India
R. N. Singh
Affiliation:
Silkworm Seed Technology Laboratory, Carmelram Post, Kodathi, Bangalore, India
M. V. Samson
Affiliation:
Silkworm Seed Technology Laboratory, Carmelram Post, Kodathi, Bangalore, India
A. Manjula
Affiliation:
Silkworm Seed Technology Laboratory, Carmelram Post, Kodathi, Bangalore, India
P. C. Santha
Affiliation:
Silkworm Seed Technology Laboratory, Carmelram Post, Kodathi, Bangalore, India
Chandrashekharaiah
Affiliation:
Silkworm Seed Technology Laboratory, Carmelram Post, Kodathi, Bangalore, India
*
* To whom all correspondence should be addressed.
Get access

Abstract

The spore replication rate of the parasitic microsporidian, Nosema bombycis, was investigated in the pupal and adult stages of two popular tropical silkworm races of India, Pure Mysore (multivoltine race) and NB18 (bivoltine race). The rate was assessed in terms of spore yield per unit weight of tissue. In pupae, spore replication in the bivoltine race was higher than that in the multivoltine race. Spore replication in both races followed a logistic pattern. Spore production increased substantially with age of moths in both races. The cephalothoracic region of each race had the highest spore concentration, especially around the wing and wing muscles.

Résumé

Le taux de multiplication de spores de la microsporidie parasite, Nosema bombycis, a été étudié chez la pupe et l'adulte de 2 variétés tropicales communes de race de ver-à-soie d'Inde, Pure Mysore (race multivoltine) et NB18 (race bivoltine). Le taux a été évalué du point de vue du rendement de spores par unité de poids de tissu. Chez les pupes, la multiplication des spores chez la race bivoltine était plus élevée que celle de la race multivoltine. Chez les 2 races, la multiplication des spores a suivi une courbe de croissance logistique. Chez les adultes de 2 races, la production de spores s'est accrue substantiellement avec l'augmentation de l'âge du papillon de nuit. La région cephalothoracique de chaque race avait une concentration maximum de spores chez les 2 races avec une très forte concentration sur les ailes et les muscles d'ailes.

Keywords

Nosema bombycisBombyx morispore replicationNosema bombycisBombyx morimultiplication de spores
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 15 , Issue 4-5 , October 1994 , pp. 427 - 431
Copyright
Copyright © ICIPE 1994

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bencel, J. J. and Undeen, A. H. (1992) Influence of temperature on developmental parameters of the parasite/host system Edhazardia aedis (Microsporida: Amblyosporidae) and Aedes aegypti (Diptera: Culicidae). J.Invertebr. Pathol. 60, 299–303.Google Scholar
Fowler, J. L. and Reeves, E. L. (1975) In vivo propagation of a microsporidian pathogenic to insects. J. Invertebr. Pathol. 25, 349–353.CrossRefGoogle Scholar
Han, M. and Watanabe, H. (1988) Transovarial transmission of two microsporidia in the silkworm, Bombyx mori, and disease occurrence in the progeny population. J. Invertebr. Pathol. 51, 41–45.CrossRefGoogle Scholar
Ishihara, R. and Fujiwara, T. (1965) The spread of pebrine within a colony of the silkworm, Bombyx mori (Linnaeus). J. Invertebr. Pathol. 7, 126–131.CrossRefGoogle ScholarPubMed
Krishnaswami, S., Narasimhanna, M. N., Suryanarayana, S. K. and Kumar, Raj S. (1973) Silkworm rearing II—Manual on Sericulture, FAO, Rome. pp. 54–58.Google Scholar
Lai, P. F. and Canning, E. U. (1980) Infectivity of a microsporidium of mosquitoes (Nosema algerae) to larval stages of Schistosoma mansoni in Biomphalaria glabrata. Int. J. Parasitol. 10, 293–301.CrossRefGoogle ScholarPubMed
Lai, P. F. and Canning, E. U. (1983) Some factors affecting spore replication of Nosema algerae (Microspora, Nosematidae) in Pieris brassicae (Lepidoptera). J. Invertebr. Pathol. 41, 20–26.CrossRefGoogle Scholar
Pilley, B. M., Canning, E. U. and Hammond, J. C. (1978) The use of micro injection procedure for large scale production of the microsporidian Nosema eurytremae in Pieris brassicae. J. Invertebr. Pathol. 32, 355–358.CrossRefGoogle Scholar
Sato, R., Kobayashi, M. and Watanabe, H. (1982) Internal ultrastructure of microsporidians isolated from the silkworm, Bombyx mori. J. Invertebr. Pathol. 40, 260–265.CrossRefGoogle Scholar
Sato, R. and Watanabe, H. (1980) Purification of mature microsporidian spores by iso-density equilibrium centrifugation. J. Seric. Sci. Jpn 49, 512–516.Google Scholar
Undeen, A. M. and Maddox, J. V. (1973) The infection of non mosquito host by injection with spores of the microsporidian Nosema algerae. J. Invertebr. Pathol. 22, 258–265.CrossRefGoogle Scholar