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Significance of the midgut bacterium Pseudomonas fluorescens on Culex vishnui (Diptera: Culicidae) larval development

Published online by Cambridge University Press:  21 December 2010

M. Roy ,
S.N. Chatterjee ,
P. Roy  and
T.K. Dangar
M. Roy
Affiliation:
Microbiology Research Unit, Parasitology Laboratory, Department of Zoology, Burdwan University, Burdwan713 104, West Bengal, India:
S.N. Chatterjee
Affiliation:
Microbiology Research Unit, Parasitology Laboratory, Department of Zoology, Burdwan University, Burdwan713 104, West Bengal, India:
P. Roy
Affiliation:
Microbiology Research Unit, Parasitology Laboratory, Department of Zoology, Burdwan University, Burdwan713 104, West Bengal, India:
T.K. Dangar*
Affiliation:
Microbiology Laboratory, Crop Production Division, Central Rice Research Institute, Cuttack753 006, Orissa, India
*
*E-mail: [email protected]
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Abstract

We investigated the role of an entomogenic gut bacterium Pseudomonas fluorescens on larval development and survival of Culex vishnui Theobald, the mosquito vector of Japanese encephalitis, which breeds exclusively in rice field water in tropical regions. The bacterium could be isolated from the midguts of third and fourth instars of the mosquito. Pseudomonas fluorescens strain 1 (Ps1) was identified as the one vital to C. vishnui larval survival. The phenotypic characteristics of this strain are described. As part of the study, antibiotics were used to cure C. vishnui larvae of Ps1; thus cured larvae did not survive when subsequently reared in sterile or antibiotic-treated rice field water or tap water. In contrast, 93.3–100% of cured larvae developed into normal adults when subsequently reared either in unsterile or in sterile rice field water or in tap water containing Ps1 bacteria (5 × 103 ml). Thus the elimination of Ps1 from breeding water could be used as a strategy to suppress the mosquito growth, as part of an innovative approach to control Japanese encephalitis.

Keywords

Culex vishnuigut bacteriaJapanese encephalitisPseudomonas fluorescensvectorpaddy ricebiological control
Type
Research Paper
Information
International Journal of Tropical Insect Science , Volume 30 , Issue 4 , December 2010 , pp. 182 - 185
Copyright
Copyright © ICIPE 2010

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References

Baumann, P., Baumann, L., Lai, C.-Y., Rouhbakhsh, D., Moran, N. A. and Clark, M. A. (1995) Genetics, physiology, and evolutionary relationships of the genus Buchnera: intracellular symbionts of aphids. Annual Review of Microbiology 49, 55–94.CrossRefGoogle ScholarPubMed
Chernysh, S., Kim, S. I., Bekker, G., Pleskach, V. A., Anikin, V. B., Platonov, V. G. and Bulet, P. (2002) Antiviral and antitumor peptides from insects. Proceedings of the National Academy of Sciences of the USA 99, 12628–12632.Google Scholar
Dillon, R. J. and Dillon, V. M. (2004) The gut bacteria of insects: non-pathogenic interactions. Annual Review of Entomology 49, 71–92.CrossRefGoogle Scholar
Dillon, R. J., Vennard, C. T., Buckling, A. and Charnley, A. K. (2005) Diversity of locust gut bacteria protects against pathogen invasion. Ecology Letters 8, 1291–1298.CrossRefGoogle Scholar
Hati, A. K. (1986) Studies on Culex vishnui and other related vectors of Japanese encephalitis in rural West Bengal. Technical Monograph, Calcutta School of Tropical Medicine, Kolkata. 120 pp.Google Scholar
Lacey, L. A. (ed.) (1997) Manual of Techniques in Insect Pathology. Academic Press, San Diego, London/Boston/New York/Sydney/Tokyo/Toronto. 409 pp.Google Scholar
Lehane, M. J., Wu, D. and Lehane, S. M. (1997) Midgut specific immune molecules are produced by the blood-sucking insect Stomoxys calcitrans. Proceedings of the National Academy of Sciences of the USA 94, 11502–11507.Google Scholar
Muthu, S. E., Nandakumar, S. and Rao, U. S. (2005) The effect of methanolic extract of Tamarindus indica Linn. on the growth of clinical isolates of Burkholderia pseudomallei. Indian Journal of Medical Research 122, 525–528.Google ScholarPubMed
Palleroni, N. J. (1984) Pseudomonadacae, pp. 141–219. In Bergey's Manual of Systematic Bacteriology (edited by Krieg, N. R.). Vol. I. Williams and Wilkins, Baltimore, Maryland.Google Scholar
Perry, R. D., Balbo, P. B., Jones, H. A., Fetherston, J. D. and DeMoll, E. (1999) Yersiniabactin from Yersinia pestis: biochemical characterization of the siderophore and its role in iron transport and regulation. Microbiology 145, 1181–1190.CrossRefGoogle ScholarPubMed
Poopathi, S. and Tyagi, B. K. (2006) The challenge of mosquito control strategies: from primordial to molecular approaches. Biotechnology and Molecular Biology Reviews 1, 51–65.Google Scholar
Quadri, L. E. N. and Ratledge, C. (2005) Iron metabolism in the tubercle bacillus and other mycobacteria, pp. 341–357. In Tuberculosis and the Tubercle Bacillus (edited by Cole, S. T., Eisenach, K. D., McMurray, D. N. and Jacobs, W. R. J.). ASM Press, Washington, District of Columbia.Google Scholar
Rajagopal, R. (2009) Beneficial interactions between insects and gut bacteria. Indian Journal of Microbiology 49, 114–119.CrossRefGoogle ScholarPubMed
Rani, R., Sharma, A., Rajagopal, R., Adak, T. and Bhatnagar, R. K. (2009) Bacterial diversity analysis of larvae and adult midgut microflora using culture-dependent and culture-independent methods in lab-reared and field-collected Anopheles stephensi – an Asian malarial vector. BMC Microbiology 9, 96(doi:10.1186/1471-2180-9-96).CrossRefGoogle ScholarPubMed
Smibert, R. and Krieg, N. R. (1995) Phenotypic testing, pp. 607–654. In Methods for General and Molecular Bacteriology (edited by Gerhardt, P., Murray, R. G. E., Wood, W. and Krieg, E.). American Society for Microbiology, Washington, District of Columbia.Google Scholar
Straif, S. C., Mbogo, C. N., Toure, A. M., Walker, E. D., Laufman, M., Toure, Y. T. and Beier, J. C. (1998) Midgut bacteria in Anopheles gambiae and An. funestus (Diptera: Culicidae) from Kenya and Mali. Journal of Medical Entomology 35, 222–226.CrossRefGoogle Scholar
Takahiro, H., Yoshitomo, K., Naruo, N., Masakazu, S. and Takema, F. (2006) Strict host–symbiont cospeciation and reductive genome evolution in insect gut bacteria. PloS Biology 4, e337.Google Scholar
Zhang, H. and Brune, A. (2004) Characterization and partial purification of proteinases from the highly alkaline midgut of the humivorous larvae of Pachnoda ephippiata (Coleoptera: Scarabaeidae). Soil Biology and Biochemistry 36, 435–442.Google Scholar