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Screening and bioassay of entomopathogenic fungi for the control of silverleaf whitefly, Bemisia argentifolli

Published online by Cambridge University Press:  19 September 2011

Adhanom Negasi ,
Bruce L. Parker  and
Michael Brownbridge
Adhanom Negasi
Affiliation:
Entomology Research Laboratory, University of Vermont, P. O. Box 53400, Burlington, VT 05405–3400
Bruce L. Parker
Affiliation:
Entomology Research Laboratory, University of Vermont, P. O. Box 53400, Burlington, VT 05405–3400
Michael Brownbridge
Affiliation:
Entomology Research Laboratory, University of Vermont, P. O. Box 53400, Burlington, VT 05405–3400
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Abstract

Isolates of Beauveria bassiana (Balsamo) Vuillemin, Paecilomyces farinosus (Holm ex SF Gray) Brown and Smith and Verticillium lecanii (Zimmermann) Viegas were evaluated for their pathogenicity to adults, eggs, first and third instars of silverleaf whitefly, Bemisia argentifolli (Bellows and Perring) (Homoptera: Aleyrodidae). Conidial dilutions were prepared in 0.05% Tween® and leaves treated by immersion in these suspensions. Assessments of nymphal infection were made 2, 3, 5, 7, and 10 days post-treatment. Infection rates in adults and eggs were made 14 days post-treatment. All isolates were pathogenic to nymphs and adults. None of the isolates were pathogenic to eggs. Isolates L3444 (P. farinosus) and L3009 (B. bassiana) were the most pathogenic to first instars and adults with LC50 and LC90 values of 7.3 × 104 and 3.1 × 106 conidia/ml, and 1.3 × 106 and 1.0 × 107 conidia/ml, respectively. Isolate FR20 (V. lecanii) was the most pathogenic to third instars with LC50 and LC90 values of 4.8 × 103 and 7.4 × 105 conidia/ml, respectively. Results imply that L3444 and L3009 are the most efficient isolates, based on their pathogenicity to first instars and adults. Results are discussed in relation to using fungi for management of silverleaf whitefly in greenhouses.

Keywords

Bemisia argentifollibioassaybiological controlentomopathogenic fungifungal isolatesBemisia argentifolliessai biologiquelutte biologiquechampignons entomopathogènesisolats fongiques
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 18 , Issue 1 , March 1998 , pp. 37 - 44
Copyright
Copyright © ICIPE 1998

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References

Aguda, R. M. and Rombach, M. C. (1986) Handling of insect fungi, pp. 117–123. In Proceedings of the Southeast Asian Regional Training Course on Microbial Control of Insect Pests and Plant Diseases in the Tropics. National Institute of Biotechnology and Applied Microbiology (BIOTECH), U. P. Los Baños, Laguna, Philippines. October 19–26, 1986. University of Philippines, Los Baños.Google Scholar
Brownbridge, M., Parker, B. L. and Skinner, M. (1992) Development of insect-killing fungi for greenhouse use: A more sustainable pest management approach, pp. 39–40. In Sustaining a Vermont Way of Life:Research and Education in Sustainable Agriculture at the University of Vermont, Plant and Soil Science Department and Extension System (Edited by Seyler, E. with assistance from Magdoff, F., Duesterburg, K., Heleba, D., Holtzman, B. and Fardleman, B.). University of Vermont.Google Scholar
Drummond, J. and Heale, J. B. (1988) Genetic studies on the inheritance of pathogenicity in Verticillium lecanii against Trialeurodes vaporariorum. J. Invertebr. Pathol. 52, 57–65.CrossRefGoogle Scholar
Drummond, J., Heale, J. B. and Gillespie, A. T. (1987) Germination and effect of reduced humidity on the expression of pathogenicity in Verticillium lecanii against the greenhouse whitefly, Trialeurodes vaporariorum. Ann. Appl. Biol. 111, 193–201.CrossRefGoogle Scholar
Ekbom, B. S. (1979) Investigation on the potential of parasitic fungus (Verticillium lecanii) for biological control of greenhouse whitefly (Trialeurodes vaporariorum). Swedish J. Agric. Res. 9, 129–138.Google Scholar
Ekbom, B. S. and Ahman, I. (1980) The fungus Verticillium fusisporum as an insect pathogen, J. Invertebr. Pathol. 36, 136–138.CrossRefGoogle Scholar
Feng, M. G. and Johnson, J. B. (1990) Relative virulence of six isolates of Beauveria bassiana on Diuraphis noxia (Homoptera: Aphididae). Environ. Entomol. 19, 785–790.CrossRefGoogle Scholar
Fransen, J. J., Winkelmann, C. and van Lenteren, J. C. (1987) The different mortality at various stages of the greenhouse whitefly, Trialeurodes vaporariorum (Homoptera: Aleyrodidae), by infection with the fungus Ashersonia aleyrodis (Deuteromycotina: Coelomycetes). J. Invertebr. Pathol. 50, 158–165.CrossRefGoogle Scholar
Hall, R. A. (1976) A bioassay of the pathogenicity of Verticillium lecanii conidiospores on the aphid, Macrosiphoniella sanborni. J. Invertebr. Pathol. 27, 41–48.CrossRefGoogle Scholar
Hall, R. A. (1985) Whitefly controlby fungi, pp. 116–118. In Biological Pest Control—The Glasshouse Experience (Edited by Hussey, N. W. and Scopes, N.). Cornell Univ. Press, Ithaca, NY.Google Scholar
Herakly, F. A. and El-Ezz, A. A. (1970) The cotton whitefly, Bemisia tabaci Genn., infesting cucurbits, in U. A. R. Agric. Res. Rev. 48, 110–118.Google Scholar
Hussey, N. W. (1985) History of biological control in protected culture, Western Europe, pp. 11–22. In Biological Pest Control—The Glasshouse Experience (Edited by Hussey, N. W. and Scopes, N.). Cornell Univ. Press, Ithaca, NY.Google Scholar
Kanagaramam, P., Hall, R. A. and Burges, H. D. (1982) Control of glasshouse whitefly, Trialeurodes vaporariorum, by an aphid isolate of the fungus Verticillium lecanii. Ann. Appl. Biol. 100, 213–219.CrossRefGoogle Scholar
Lopez-Avila, A. and Cock, M. J. W. (1986) Economicy damage, pp. 51–54. In Bemisia tabaci—A Literature Survey on the Cotton Whitefly with Annotated Bibliography (Edited by Cock, M. J.). CAB International Institute of Biological Control, Silwood Park, Ascot, Berks SL5 7PY UK.Google Scholar
Matthews, G. A. (1986) Overview of chemical control with special reference to cotton crops, pp. 51–54. In Bemisia tabaci—A Literature Survey on the Cotton Whitefly with Annotated Bibliography (Edited by Cock, M. J. ). CAB International Institute of Biological Control, Silwood Park, Ascot, Berks SL5 7PY UK.Google Scholar
McHugh, J. B. (1991) Yes, you can win the war on whiteflies. Greenhouse Grower, Spring, 1–2.Google Scholar
Ondieki, J. J. (1975) Diseases and pests of passion fruit in Kenya. Acta Horticulturae 49, 291–293.CrossRefGoogle Scholar
Osborne, L. S. and Landa, Z. (1992) Biological control of whitefly with entomopathogenic fungi. Florida Entomol. 75, 456–471.CrossRefGoogle Scholar
Reinecke, P. (1990) Biological control products: Demands of industry for successful development, pp. 99–108. In Pesticides and Alternatives: Innovative Chemical and Biological Approaches to Pest Control (Edited by Casida, J. E.). Elsevier Science Publisher. Amsterdam, Oxford, NY.Google Scholar
Sanderson, J. P. and Ferrentino, G. W. (1991) Whiteflies 101: A primer on biology and control. Grower Talks 55, 48–61.Google Scholar
SAS Institute (1987) SAS User's Guide: Statistics. SAS Institute, Cary, NC.Google Scholar
Shaheen, A. H. (1977) Survey of pests attacking tomato in Egypt with some ecological notes. Agric. Res. Rev. 55, 49–57.Google Scholar
van der Schaaf, D. A., Ravensberg, W. J. and Malais, M. (1991) Verticullum lecanii as a microbial insecticide against whitefly, pp. 120–123. In Proc. Third European Meeting on “Microbial Control of Pests” (Edited by Smits, P. H.). Wageningen, The Netherlands, 24–27 February 1991. IOBC/WPRS Bulletin XIV/7.Google Scholar
van Lenteren, J. C. and Noldus, L. P. J. (1990) White fly plant relationships: Behavioral and ecological aspects. In White flies: Their Bionomics, Pest Status and Management, pp. 47–89 (Edited by Gerling, D.). Intercept Ltd., Andover, Hants, UK.Google Scholar