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The relationship between primary metabolites in reproductive structures of cowpea Vigna unguiculata (Fabaceae: Papilionidae) cultivars and field resistance to the flower bud thrips Megalurothrips sjostedti (Thysanoptera: Thripidae)

Published online by Cambridge University Press:  28 February 2007

O.Y. Alabi ,
J.A. Odebiyi  and
M. Tamò
O.Y. Alabi*
Affiliation:
International Institute of Tropical Agriculture, Oyo Road PMB 5320, Ibadan, Nigeria
J.A. Odebiyi
Affiliation:
Department of Crop Protection and Environmental Biology, Entomology Unit, University of Ibadan, Nigeria
M. Tamò
Affiliation:
International Institute of Tropical Agriculture, Biological Control Centre for Africa, Cotonou, Bénin, South Africa
*
*E-mail: [email protected] [email protected]
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Abstract

Preliminary screening of germplasm from the International Institute of Tropical Agriculture (IITA) showed that a number of cowpea Vigna unguiculata (L). Walp. cultivars have potential for resistance to the flower bud thrips, Megalurothrips sjostedti (Trybom). In an earlier study, 10 cultivars from this germplasm were selected and the mechanisms of resistance determined. Therefore, in this study, the basis of resistance operating in the cultivars was elucidated during the first and second planting seasons of 1998. Cowpea cultivars were analysed for primary metabolites (total protein content and glucose contents) to study their relationship with resistance parameters of M. sjostedti under field conditions. Total protein and glucose contents varied significantly (P<0.01) in floral buds and flowers of the different cultivars, while in racemes there were little or no significant differences. Highly significant negative correlation coefficients were obtained between total protein contents in reproductive structures and resistance parameters, especially during the second season, regardless of cowpea cultivar, indicating that quality of total protein content plays a significant role in cowpea resistance to M. sjostedti. Significant (P<0.05) correlation between damage indices and glucose content in Vita 7 and Kpodjiguegue is responsible for their susceptibility to M. sjostedti damage. Furthermore, presence of a unique protein band (20.1 kDa) in Moussa local, Sanzibanili and Sewe cultivars could be associated with resistance to flower bud thrips, regardless of quantity of total protein content in these and other test cultivars. Similarly, the specific band just above the 94 kDa in Vita 7 could be responsible for its susceptibility to M. sjostedti.

Keywords

Megalurothrips sjostedtiVigna unguiculatatotal proteinglucose contentreproductive structuresresistance
Type
Research Article
Information
International Journal of Tropical Insect Science , Volume 26 , Issue 1 , March 2006 , pp. 8 - 15
Copyright
Copyright © ICIPE 2006

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References

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