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Protein synthesis in the salivary glands of some bugs: An autoradiographic study of (3H) glycine incorporation

Published online by Cambridge University Press:  19 September 2011

P. R. Yadav
P. R. Yadav*
Affiliation:
Department of Zoology, Banaras Hindu University, Varanasi-221 005, India
*
*Silkworm Seed Technology Laboratory, Kodathi, Carmelaram Post Bangalore-560035, India.
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Abstract

Autoradiographic observations using (3H) glycine in the salivary glands of Lethocerus indicus, Laccotrephes rubra and Antilochus coqueberti indicated synthetic activity being performed by the glandular epithelia in all the bugs at short incubation periods. Long incubation periods showed that the site of heavy labelling shifts from outside inwards, with progressive incubation timings, namely from the basal borders and eventually into the lumen. This shifting of the labelling from outside inwards suggests the glandular epithelium is also capable of transporting some of the haemolymph proteins into the gland in all the species and thus indicating heterosynthetic origin of saliva.

Résumé

Des observations autoradographiques utilisant Glycine (3H) au niveau des glandes salivaires de Lethocerus indicus, Laccotrephes rubra et Antilochus coqueberti ont montré une activité de synthèse par l'épithélium glandulaire après une courte période d'incubation. Au cours de longues périodes on remarque au moyen d'un chronométrage progressif du temps d'incubation que le site de concentration du produit de secrétion marqué se déplace de l'extérieur vers l'intérieur surtout de la membrane basale et éventuellement vers la lumière. Ce déplacement de la substance marquée vers l'intérieur suggère que l'épithélium glandulaire est aussi capable de transporter des proteines de l'hémolymphe à la glande dans toutes les espèces indiquant ainsi une origine hétérosynthétique de la salive.

Keywords

Salivary glandsLethocerus indicusLaccotrephes rubraAntilochus coquebertiautoradiography(3H) glycineprotein metabolism
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 13 , Issue 1 , February 1992 , pp. 49 - 58
Copyright
Copyright © ICIPE 1992

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References

REFERENCES

Akimoto, S. (1981) Gall formation by Eriosoma fundatries and gall parasitism in Eriosoma yanegi (Homoptera: Pephigidae). Kontya 49, 426–436.Google Scholar
Baptist, B. A. (1941) The morphology and physiology of the salivary glands of Hemiptera-Heteroptera. Q. J. Micros. Soc. 83, 91–139.Google Scholar
Beardsley, J.W. Jr (1984) Gall forming Coccoidea. In Biology of Gall Insects (Edited by Ananthakrishnan, T. N.), pp. 79–106. Oxford & IBH Publishing Co. India.Google Scholar
Brouwers, E. E. M (1982) Measurement of hypopharyngeal gland activity in the honey bee. J. Apic. Res. 21, 193–198.CrossRefGoogle Scholar
Crailsheim, K. (1986) Dependence of protein metabolism on age and season in the honey bee (Apis mellifica carnica). J. Insect. Physiol. 32, 629–634.CrossRefGoogle Scholar
Happ, G. W. and Yuncker, C. (1978) Patterns of leucine incorporation in the spermathecal accessory glands of the postecdysial adult female Tenebrio molitor. J. Insect Physiol. 24, 417–421.CrossRefGoogle Scholar
Haridass, E.T. and Ananthakrishnan, T.N. (1981) Functional morphology of the salivary system in some Reduviidae (Insects: Heteroptera). Proc. Indian Acad. Sci. (Anim. Sci.) 90, 145–160.CrossRefGoogle Scholar
Hirumi, H. and Maramorosch, K. (1969) Mycoplasma like bodies in the salivary glands of insect vectors carrying in the aster yellow agent. J. Virol. 3, 82–84.CrossRefGoogle Scholar
Hodkinson, I. D. (1984) The biology and ecology of the gall forming Psylloidea (Homoptera). In Biology of Gall Insects (Edited by Ananthakrishnan, T. N.), pp. 59–77. Oxford & IBH Publishing Co. India.Google Scholar
Kumar, D. (1978) Histophysiological studies of the salivary apparatus of Indian Hemiptera: Morphological, histological, histochemical, autoradiographic and electron microscopic investigations. Ph.D. thesis, Banaras Hindu University, Varanasi, India.Google Scholar
Laufer, H. and Nakase, Y. (1965) The salivary gland secretion and its relation to chromosomal puffing in the Dipteran, Chironomus thummi. Proc. Nat. Acad. Sci. USA, 53, 511–516.CrossRefGoogle Scholar
Louis, C., Drif, L. and Vago, C. (1986) Mise evidence et étude ultrastructurale de procaryotes de type Rickettsien dans les glandes salivaires de Tritomidae (Heteroptera). Ann. Soc. entomol. France (N. S.) 22, 153–162.CrossRefGoogle Scholar
Miles, P. W. (1967) Studies on the salivary physiology of plant bugs: transport from haemolymph to saliva. J. Insect Physiol. 13, 1787–1801.CrossRefGoogle Scholar
Miles, P. W. (1969) Incorporation and metabolism of cysteine in the haemolymph and saliva of a plant bug. Aust. J. Biol. Sci. 22, 1271–1276.CrossRefGoogle Scholar
Miles, P.W. (1972) The saliva of Hemiptera. Adv. Insect Physiol. 9, 183–225.CrossRefGoogle Scholar
Miles, P.W. and Slowiak, D. (1970) Transport of whole protein molecules from blood to saliva of a plant bug. Experientia 26, 611.CrossRefGoogle ScholarPubMed
Miller, N. C. E. (1971) The Biology of the Heteroptera. Second (revised) ed. Hampton (England) E. W. Classey.Google Scholar
Pirone, T. P. (1969) Mechanism of transmission of stylet-borne viruses. In Viruses, Vectors and Vegetation (Edited by Maramorosch, K.), pp. 199–210. Interscience Publishers, New York.Google Scholar
Postlethwait, J. H. and Giorgi, F. (1985) Vitellogenesis in insects. In Developmental Biology: A Comprehensive Synthesis, Vol. I Oogenesis (Edited by Browder, L.), pp. 85–126. Plenum Press, New York.Google Scholar
Schin, K. S. and Clever, U. (1968) Ultrastractural and cytochemical studies on the salivary gland regression in Chironomus thummi. Z. Zellforsch 3, 81–94.Google Scholar
Sridevi, R. and Ray, A. (1988) Protein synthesis in the male accessory reproductive glands of Spodoptera litura (Noctuidae: Lepidoptera). Insect Sci. Applic. 9, 635–637.Google Scholar
Wool, D. (1984) Gall forming aphids. In Biology of Gall Insects (Edited by Ananthakrishnan, T. N.), pp. 11–58. Oxford & IBH Publishing Co. India.Google Scholar
Yadav, P. R. (1988) Studies on the salivary apparatus and saliva of some Hemiptera. Ph.D. thesis, Banaras Hindu University, India.Google Scholar