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Mode of action of Bacillus thuringiensis and nuclear polyhedrosis virus in the larvae of Spodoptera littoralis (Boisd.)

Published online by Cambridge University Press:  19 September 2011

H. S. Salama ,
A. Sharaby  and
M. Magd El-Din
H. S. Salama
Affiliation:
Department of Biology, Faculty of Medicine, King Abdul-Aziz University, Jeddah, SA
A. Sharaby
Affiliation:
Department of Pests and Plant Protection, National Research Centre, Dokki, Cairo, Egypt
M. Magd El-Din
Affiliation:
Department of Pests and Plant Protection, National Research Centre, Dokki, Cairo, Egypt
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Abstract

An additive effect was obtained after treatment of Spodoptera littoralis (Boisd.) larvae with a combination of Bacillus thuringiensis and nuclear polyhedrosis virus (NPVs). The NPVs was the more pathogenic followed by B. t., cytopathologically. Simultaneous application of B. t. and NPVs affected the midgut cells by causing a very specific appearance of cell hypertrophy with some deposits of B. t. crystals on the fourth day of infection as shown by scanning electron microscopy. Transmission electron microscopy showed that the vegetative cells and spores of B. t. entered the midgut epithelial cells followed by the virus. It is possible that the initial destruction caused by the B. t. toxin facilitated penetration and entry of the virus.

Résumé

L'effet additionnel etait obtenue apres un traitement combine du Bacillus thuringiensis et le virus nuclear polyhedrosis (NPVs) contre la larve Spodoptera littoralis. Le viruse NPVs etait le plus pathogenique puis etait B. t. Les effets cytopathologiques du traitement combine du B. t. et NPVs sur les cellules du midgutte (midgut) hypertrophie nombre de cristate de B. t. ont ete observees par le microscope (scanning electron microscopy). La transmission microscopicale electronique a montre que les cellules vegetatives et les spores du B. t. ont entre les cellules epitheliales du midgutte, ensuite le virus est rentre. II est possible que les premieres destructions qui soni causes par le toxin de B. t. facilitent la penetration et I'entree de virus.

Keywords

Additive effectnuclear polyhedrosis virusBacillus thuringiensisSpodoptera littoralis
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 14 , Issue 4 , August 1993 , pp. 537 - 543
Copyright
Copyright © ICIPE 1993

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