Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-04T19:50:13.005Z Has data issue: false hasContentIssue false
  • English
  • Français

Microorganisms associated with tsetse in nature: Preliminary results on isolation, identification and pathogenicity

Published online by Cambridge University Press:  19 September 2011

Godwin P. Kaaya  and
Matilda A. Okech
Godwin P. Kaaya
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi, Kenya
Matilda A. Okech
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi, Kenya
Get access

Abstract

Six genera of bacteria: Serratia, Providencia, Enterobacter, Aeromonas, Cedecea and Bacillus were isolated from field-collected G. pallidipes. Four genera: Serratia, Providencia, Enterobacter and Aeromonas were isolated from insectary-reared Glossina morsitans morsitans. All bacteria except, Bacillus spp. and Providencia alcalifaciens were pathogenic for teneral G. m. morsitans. Bacteria isolated from insectary-reared tsetse were more pathogenic than those isolated from the field. Fungi of three genera were also isolated from field-collected adult G. pallidipes, Penicillium, Aspergillus and Fusarium. Five genera, Aspergillus, Penicillium, Rhizopus, Mucor and Trichoderma were isolated from field-collected G. pallidipes pupae. The fungi, however, were only mildly pathogenic for adult tsetse and were not pathogenic for pupae.

Résumé

Six genres de bactéries: Serratia, Providencia, Enterobacter, Aeromonas, Cedecea et Bacillus ont été isolés à partir des mouches tsé-tsé adultes G. pallidipes récoltées sur le terrain. Quatre genres: Serratia, Providencia, Enterobacter et Aeromonas étaient isolés à partir de G. m. morsitans élevés à l'insectarium. Les testes de pathogénicité ont révelé que la pluspart des bactéries, à l'exception de Bacillus spp et Providencia alcalifaciens, étaient très pathogènes aux jeunes mouches tsé-tsé. Les bactéries isolées à partir des mouches tsé-tsé élevées à l'insectarium étaient très pathogènes que celles isolées des mouches tsé-tsé récoltées sur le terrain. Trois genres de champignons ont été isoles à partir des adultes de G. pallidipes récoltés sur le terrain. Ce sont Pénicillium, Aspergillus et Fusarium. Cinq genres: Aspergillus, Penicillium, Rhizopus, Mucor et Trichoderma été isolés a partir des pupes de G. pallidipes récoltées sur le terrain. Toutefois, les champignons ont montré une très faible pathogènicité aux mouches tsé-tsé adultes et étaient non pathogèns aux pupes.

Keywords

Glossina morsitans morsitansG. pallidipesSerratiaProvidenciaEnterobacterCedeceaBacillusPenicilliumAspergillusFusariumRhizopusMucorTrichodermapathogenicityGlossina morsitans morsitansG. pallidipesSerratiaProvidenciaEnterobacterCedeceaBacillusPenicilliumAspergillusFusariumRhizopusMucorTrichodermapathogénicité
Type
Research Article
Information
International Journal of Tropical Insect Science , Volume 11 , Issue 3 , June 1990 , pp. 443 - 448
Copyright
Copyright © ICIPE 1990

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Carlberg, G. (1986) Bacillus thuringiensis and microbial control of flies. Mircern J. 2, 267–274.CrossRefGoogle Scholar
Calberg, G., Kihamia, C. M. and Minjas, J. (1985) Microbial control of flies in latrines in Dar-es-Salaam with a Bacillus thuringiensis (serotype I) preparation, Muscabac. Mircern J. 1, 33–44.CrossRefGoogle Scholar
Goldberg, L. J. and Margalit, J. (1977) A bacterial spore demonstrating rapid larvicidal activity against Anopheles sergentii, Urantaenia unguiculata, Culex univittatus, Aedes aegypti and Culex pipiens. Mosq. News 37, 355–358.Google Scholar
Gruvel, J. (1970) Elevage de G. tachinoides W. dans les conditions tropicales au Tchad. Proc. 1st. Symp. on Tsetse Fly Breeding under Laboratory Conditions andits Practical Application. Lisbon, 22–23 April, 1969, pp. 117–123.Google Scholar
Kaaya, G. P. (1989) Glossina morsitans morsitans: Mortalities caused in adults by experimental infection with entomopathogenic fungi. Acta Trop. 46, 107–114.CrossRefGoogle ScholarPubMed
Kaaya, G. P. and Darji, N. (1989) Mortality in adult tsetse, Glossina morsitans morsitans caused by entomopathogenic bacteria. J. Invert. Pathol. 54, 32–38.CrossRefGoogle ScholarPubMed
Kaaya, G. P. and Okech, M. A. (1990) Horizontal transmission of mycotic infection in adult tsetse, Glossina morsitans morsitans. Entomophaga (in press).CrossRefGoogle Scholar
Lacey, L. A., Escaffre, H., Philippon, B., Seketeli, A. and Guillet, P. (1982) Large river treatment with Bacillus thuringiensis (H-14) for the control of Simulium damnosum s.l. in the Onchocerciasis Control Programme. Tropenmed. Parasitol. 33, 97–101.Google ScholarPubMed
Nobre, G. and Santos, A. M. T. (1970) The use of antibiotics in artificial breeding of Glossina morsitans: A bacteriological study. Proc. 1st Int. Symp. on Tsetse Fly Breeding under Laboratory Conditions and its Practical Application, Lisbon, 22–23 April 1969, pp. 335–340.Google Scholar
Oliveira, J. C. and Nobre, G. (1970) Bacteriological and mycological study of a laboratory-bred Glossina colony. Proc. 1st Int. Symp. on Tsetse Fly Breeding under Laboratory Conditions and its Practical Application. Lisbon, 22–23 April 1969, pp. 341–348.Google Scholar
Poinar, G. O. Jr, Wassink, H. J. M., Leegwater Van den Linden, E. M. and Van der Geest, L. P. S. (1979) Serratia marcescens as a pathogen of tsetse flies. Acta Trop. 36, 223–227.Google ScholarPubMed
Roubaud, E. and Treillard, M. (1935) Un coccobacille pathogène pour les mouches tsétsé. C. R. Acad. Sci. (Paris) 201, 304–306.Google Scholar
Singer, S. (1980) Bacillus sphaericus for the control of mosquitoes. Biotechnol. Bioeng. 22, 1335–1355.CrossRefGoogle Scholar
Vey, A. (1971) Recherches sur les champignons pathogènes pour les glossines. Etudes sur Glossina fusca congolensis Newst. et Evans en République Centrafricaine. Rev. Elev. Méd. Vét. Pays Trop. 24, 577–579.CrossRefGoogle Scholar