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Influence of thyroxine on ovarian glycogen of Bombyx mori L. (Race Nistari) during ontogenesis

Published online by Cambridge University Press:  19 September 2011

Anathbandhu Chauduri  and
A. K. Medda
Anathbandhu Chauduri
Affiliation:
Department of Animal Physiology, Bose Institute, Kankurgachi, Calcutta-700054, India
A. K. Medda
Affiliation:
Department of Animal Physiology, Bose Institute, Kankurgachi, Calcutta-700054, India
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Abstract

Normal variations of ovarian glycogen content and the effect of different doses of thyroxine (T4) on this cellular constituent were investigated from 3rd day of fifth larval instar to adult emergence in Bombyx mori L. Ovarian glycogen remained higher during fifth larval stage with a peak on 7th day. At the end of 5th larval stage the glycogen content declined and this lower level continued upto 4th day of pupal age. Thereafter, this cellular constituent again sharply increased and reached a peak on pupal day 6, followed by a fall upto adult emergence. A single injection of different doses (0.5, 1, 2 and 5 μg/g body weight) of thyroxine, caused a significant decrease in glycogen content of ovary, during larval stage and an increase during pupal and adult stages of development with few exceptions. Thyroxine at the doses of 0.1 and 0.25 μg/g remained ineffective throughout the study period with an exception on day 2 after injection. The specific pattern of variation of the ovarian glycogen content as observed during normal stages of development was not altered by thyroxin though this hormone changed the level of glycogen. Thus, the results indicated that both glycogenolysis and glycogenesis occur in ovary after thyroxine treatment.

Résumé

Des variations normales de la teneur du glycogène ovarien et l'effet de différentes concentrations de thyroxine (T4) sur ce constituant cellulaire ont été étudiés du 3ème jour du 5ème stade larvaire de Bombyx mori L. jusqu'à émergence de l'adulte. Le niveau de glycogène ovarien était resté très élévé durante le 5ème stade larvaire, avec un pic au 7ème jour. A la fin du 5ème stade larvaire, la teneur en glycogène a brutalement decline et ce bas niveau a persisté jusqu'àu 4ème jour du stade pupal. Par le suite, ce constituant cellulaire a de nouveau brusquement remonté pour atteindre un pic au 6ème jour de la pupe, suivi de son déclin jusqu' à l'émergence de l'adulte.

Une injection unique de différentes concentrations de thyroxine (0.1, 1, 2 on 5 μg/g/poids corporel) a cause une reduction importante de la teneur en glycogène ovarien au cours du stade larvaire et son augmentation durant les stades pupal et adulte, avec quelques exceptions. La thyroxine aux concentrations de 0.1 et 0.25 μg/g est restée sans effet tout au long de la période d'étude, excepté au 2ème jour après injection. L'évolution particulière de la variation du glycogène ovarien telle qu'observée durant les différents stades de developpement n'a pas été influençée par la thyroxine, quoique cette hormone ait modifié le niveau glycogène. De ce fait, les résultats ont indiqué que la glycogénolyse et la glycogenese se poursuivent dans l'ovaire après traitement à la thyroxine.

Keywords

Bombyx moriglycogenovarythyroxineBombyx moriglycogèneovairethyroxine
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 14 , Issue 5-6 , December 1993 , pp. 621 - 626
Copyright
Copyright © ICIPE 1993

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