Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-28T05:36:12.963Z Has data issue: false hasContentIssue false
  • English
  • Français

Identifying triatomine symbiont Rhodococcus rhodnii as intestinal bacteria from Rhodnius ecuadoriensis (Hemiptera: Reduviidae) laboratory insects

Published online by Cambridge University Press:  07 March 2011

Janeth Rodríguez ,
Paula Pavía ,
Marleny Montilla  and
Concepción J. Puerta
Janeth Rodríguez
Affiliation:
Laboratorio de Parasitología Molecular, Departamento de Microbiología, Facultad de Ciencias, Universidad Javeriana, Carrera 7, #43-82, Bogotá, Colombia
Paula Pavía
Affiliation:
Laboratorio de Parasitología Molecular, Departamento de Microbiología, Facultad de Ciencias, Universidad Javeriana, Carrera 7, #43-82, Bogotá, Colombia
Marleny Montilla
Affiliation:
Grupo de Parasitología, Instituto Nacional de Salud, Avenida Calle 26, #51-20, Bogotá, Colombia
Concepción J. Puerta*
Affiliation:
Laboratorio de Parasitología Molecular, Departamento de Microbiología, Facultad de Ciencias, Universidad Javeriana, Carrera 7, #43-82, Bogotá, Colombia
*
*E-mail: [email protected]
Get access

Abstract

The Nocardiaceae family members are symbiont bacteria from triatomine insects. The aim of this study was to detect the presence of Nocardiaceae family bacteria in Rhodnius ecuadoriensis intestinal bacterial flora. Bacterial colonies were isolated from the intestinal content from all insects; they had pink or coral pink pigmentation corresponding to Gram-positive coccobacilli. These isolates' biochemical identification by catalase and urease tests and the assimilation of different carbon sources led to Rhodococcus rhodnii being identified. This result was confirmed by 16S ribosomal ribonucleic acid gene sequencing. Additional studies are required to establish the symbiont role of this bacterium in R. ecuadoriensis as it has in Rhodnius prolixus.

Keywords

Rhodococcus rhodniiRhodnius ecuadoriensisChagas disease
Type
Research Paper
Information
International Journal of Tropical Insect Science , Volume 31 , Issue 1-2 , June 2011 , pp. 34 - 37
Copyright
Copyright © ICIPE 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Azambuja, P., Feder, D. and Garcia, E. S. (2004) Isolation of Serratia marcescens in the midgut of Rhodnius prolixus: impact on the establishment of the parasite Trypanosoma cruzi in the vector. Experimental Parasitology 107, 89–96.CrossRefGoogle ScholarPubMed
Beard, C. B., Dotson, E. M., Pennington, P. M., Eichler, S., Cordon-Rosales, C. and Durvasula, R. V. (2001) Bacterial symbiosis and paratransgenic control of vector-borne Chagas disease. International Journal for Parasitology 31, 621–627.CrossRefGoogle ScholarPubMed
Durvasula, R. V., Sundaram, R. K., Kirsch, P., Hurwitz, I., Crawford, C. V., Dotson, E. and Beard, C. B. (2008) Genetic transformation of a corynebacterial symbiont from the Chagas disease vector Triatoma infestans. Experimental Parasitology 119, 94–98.CrossRefGoogle ScholarPubMed
Eichler, S. and Schaub, G. A. (2002) Development of symbiosis in triatomine bugs and the effects of infection with trypanosomatids. Experimental Parasitology 100, 17–27.CrossRefGoogle ScholarPubMed
Grijalva, M. J., Palomeque, F. S., Villacís, A. G., Black, C. L. and Arcos-Terán, L. (2010) Absence of domestic triatomine colonies in an area of the coastal region of Ecuador where Chagas disease is endemic. Memorias Do Instituto Oswaldo Cruz 105, 677–681.CrossRefGoogle Scholar
Pavía, P., Calderon, C. and Puerta, C. J. (2005) Diferenciación de especies de Rhodococcus mediante una prueba de PCR-RFLP basada en los genes codificantes para la subunidad 16S ribosomal. Nova 3, 14–20.CrossRefGoogle Scholar
Rodríguez, J., Pavía, P., Calderon, C. and Montilla, M. (2007) Estandarización de una prueba de PCR-RFLP para la identificación de Rhodococcus rhodnii en insectos triatominos. Universitas Scientiarum 12, 11–22.Google Scholar
Saitou, N. and Nei, M. (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4, 406–425.Google Scholar
Vallejo, G. A., Guhl, F. and Schaub, G. A. (2009) Triatominae – Trypanosoma cruzi/T. rangeli: vector–parasite interactions. Acta Tropica 110, 137–147.CrossRefGoogle ScholarPubMed
Villacís, A. G., Arcos-Terán, L. and Grijalva, M. J. (2008) Life cycle, feeding and defecation patterns of Rhodnius ecuadoriensis (Lent & León 1958) (Hemiptera: Reduviidae: Triatominae) under laboratory conditions. Memorias Do Instituto Oswaldo Cruz 103, 690–695.CrossRefGoogle ScholarPubMed
Villacís, A. G., Grijalva, M. J. and Catalá, S. S. (2010) Phenotypic variability of Rhodnius ecuadoriensis populations at the Ecuadorian central and southern Andean region. Journal of Medical Entomology 47, 1034–1043.CrossRefGoogle ScholarPubMed
World Health Organization (WHO) (2007) Report of the Scientific Working Group on Chagas disease. Buenos Aires, Argentina, 17–20 April 2005. Update July 2007. World Health Organization, Geneva. Available at:http://www.who.int/tdrold/diseases/chagas/swg_chagas.pdf.Google Scholar
Yassin, A. F. (2005) Rhodococcus triatomae sp. nov., isolated from a blood-sucking bug. International Journal of Systematic and Evolutionary Microbiology 55, 1575–1579.CrossRefGoogle ScholarPubMed