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Evaluation of Bacillus thuringiensis H-14 isolates from Nigerian soils for use in mosquito control

Published online by Cambridge University Press:  19 September 2011

Jason A. N. Obeta
Affiliation:
Department of Life Sciences, Ben Gurion University of the Negev P. O. B. 653, Beer Sheva 84105, Israel
Arieh Zaritsky
Affiliation:
Department of Life Sciences, Ben Gurion University of the Negev P. O. B. 653, Beer Sheva 84105, Israel
Zeev Barak
Affiliation:
Department of Life Sciences, Ben Gurion University of the Negev P. O. B. 653, Beer Sheva 84105, Israel
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Abstract

Eleven mosquitocidal Bacillus thuringiensis isolates from Nigerian soils were screened for the degree of toxicity against Aedes aegypti larvae. The six most larvicidal isolates, code-named OBGI, OBG8, BUS4, BAR3, GSC3 and GNA13, were identified as subsp. israelensis (B.t.i). Spores of the six isolates of a B. t. i. from a commercial powder (R-153–78, Roger Bellon Laboratory, Belgium) and of IPS 82 (Institut Pasteur (Standards)) were subjected to ultraviolet irradiation (280–350 nm) for up to 75 min. After irradiation, the toxicity of OBG8, OBGI, BUS4, and BAR3 was higher than that of R-153–78 but lower than that of IPS 82.

Résumé

Onze isolats (aux propriétés anti-moustiques) de Bacillus thuringiensis provenant des sols nigérians ont été passés au crible concernant leur degré de toxicité a l'égard des larves d'Aedes aegypti. Les 6 isolats ayant les plus fortes activités larvicides et désignés sous les codes OBG1, OBG8, BUS4, BAR3, GSC3 et GNA13, ont été identifiés en tant que sous-espèces israelensis (B. t. i.). Les spores des 6 isolats d'une B. t. i. provenant d'une poudre commerçiale (R-153–78; Laboratoire Roger Bellon, Belgique) et celle du IPS 82 (Institut Pasteur (Standards)), ont été soumis à l'irradiation aux ultra-violets (280–350 nm) pendant 75 min. Aprés irradiation, la toxicité de OBG8, OBG1, BUS4 et BAR3 a été supérieure à celle de R-153–78, mais inférieure à celle de IPS 82.

Type
Research Articles
Copyright
Copyright © ICIPE 1994

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