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Electrophoretic analysis of Trypanosoma brucei sub-group stocks from cattle, tsetse and patients from Lambwe Valley, Western Kenya

Published online by Cambridge University Press:  19 September 2011

L. H. Otieno ,
N. Darji  and
P. Onyango
L. H. Otieno
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi, Kenya
N. Darji
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi, Kenya
P. Onyango
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi, Kenya
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Abstract

Trypanosoma brucei stocks (43) isolated from man, cattle and tsetse, all coming from a small area of Lambwe Valley, in Kenya were characterized using isoenzyme analysis. The tsetse isolates revealed the largest number of zymodemes, indicating a lot of heterogeneity among these isolates. Cluster analysis of all the trypanosomes characterized showed that the organisms could be grouped into three distinct trypanosome types; human, cattle and tsetse. The human type was represented in ail the three groups. This was not the case either with the cattle nor the tsetse T. brucei type. T. brucei zymodeme which was found to be predominant during the 1980 outbreak of sleeping sickness was not observed this time round. It is suggested that this zymodeme was either unstable and had changed or it had been eliminated during the insecticides ground spraying operations against G. pallidipes. It is concluded that the heterogeneity observed among the fly isolates is an adaptive mechanism for survival of trypanosomes.

Résumé

Les provisions de 43 T. brucei ont été isolés de humaines, les bétail et les tsé-tsé entre 1985 et 1987, dans la vallée du Lambwe, au Kenya. L'analyse de l'isoenzyme de 11 enzyme a révélée un hétérogénéité considérable entre tous les échantillons, et les échantillons de tsé-tsé étaient les plus diverses. L'analyse de groupe de les relations entre les échantillons ont indiques que les organisms peuvent être grouper en trois types majeurs en correspondant de leurs origins dans les humaines, les cheveaux et les tsé-tsé. Le type humain, qui était représente dans tous les trois groupes, était les moins différent. Au contraire, la plupart des échantillons de cheveaux et de tsé-tsé ont étaient représentés dans les autres groupes. Le zymodème precis de T. brucei, qui existait pendant les manifestation de la maladie du someil, n'est pas observé. Ce zymodème peut avoir changé ce isoenzyme, ou il est éliminé par le groupe récent de l'opération de vaporiser l'insecticide contre G. pallidipes. En gise de conclusion, l'hétérogénéité qui était observé entre les échantillons de mouch est un mechanism adaptive pour la survivance de ce trypanosome.

Keywords

Trypanosoma bruceiisoenzymes cluster analysisheterogeneitytsetsecattleman
Type
Research Article
Information
International Journal of Tropical Insect Science , Volume 11 , Issue 3 , June 1990 , pp. 281 - 287
Copyright
Copyright © ICIPE 1990

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