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Effect of protein fractions isolated from cereal grains on the development and survival of the cowpea weevil, Callosobruchus maculatus

Published online by Cambridge University Press:  19 September 2011

L. B. S. Andrade ,
V. M. Q. Flores ,
M. P. Sales ,
R. A. Morais ,
A. Blanco-Labra  and
J. Xavier-Filho
L. B. S. Andrade
Affiliation:
Grupo de Biotecnologia de Plantas Cultivadas, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal 1065, 60001-970 Fortaleza, Ceará, Brazil
V. M. Q. Flores
Affiliation:
Grupo de Biotecnologia de Plantas Cultivadas, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal 1065, 60001-970 Fortaleza, Ceará, Brazil
M. P. Sales
Affiliation:
Grupo de Biotecnologia de Plantas Cultivadas, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal 1065, 60001-970 Fortaleza, Ceará, Brazil
R. A. Morais
Affiliation:
Grupo de Biotecnologia de Plantas Cultivadas, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal 1065, 60001-970 Fortaleza, Ceará, Brazil
A. Blanco-Labra
Affiliation:
Centro de Investigacion y de Estudios Avanzados del I.P.N—Unidad Irapuato, Carretera Irapuato-Leon, Irapuato, Gto, Mexico
J. Xavier-Filho
Affiliation:
Grupo de Biotecnologia de Plantas Cultivadas, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal 1065, 60001-970 Fortaleza, Ceará, Brazil
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Abstract

The effect of protein fractions isolated from cereal grains on the development and survival of the bean weevil, Callosobruchus maculatus, was investigated using wheat, rice and maize. These cereals were fractionated into albumins, globulins, prolamins, glutelins and a residue. The fractions were incorporated in artificial seeds which were infested by C. maculatus. Survival (as indicated by LD50) and development (as indicated by WD50) of 20-day-larvae of the bruchid were negatively affected by most of the fractions. Rice globulins were the most detrimental and affected both the survival and development of the larvae, while wheat albumins and globulins had a stronger effect on larval development than on survival. A previously isolated maize α-amylase/trypsin inhibitor showed an LD50 of 1.5% to Callosobruchus maculatus.

Résumé

L'effet des fractions protéiniques des semences de blé de riz et de maïs a été étudié sur le développement et la survie du bruchide du niébé, Callosobruchus maculatus. Les céréales ont été fractionnées en albumines, globulines, prolamines, glutélines et en un résidu, avec les fractions incorporées dans des semences artificielles infestées du bruchide, Callosobruchus maculatus. La survie (telle qu'indiquée par la CL50) et le développement (tel qu'indiqué par WD50) des larves a âgées de 20 jours ont été négativement affectés par la plupart des fractions. Les globulines du riz ont été les plus nocives, affectant à la fois la survie et le développement des larves, tandis que l'effet des albumines et de globulines du blé a été plus marqué sur le développement larvaire que sur la survie. Un inhibiteur de la α-amylase/trypsine auparavant isolé du maïs, a révélé une CL50 de 1,5% sur Callosobruchus maculatus.

Keywords

cowpea weevilCallosobruchus maculatuswheatricemaizecereal grainsprotein fractionsinsecticidal proteinsbruchide du niébéCallosobruchus maculatusblérizmaïssemences de céréalesfractions protéiniquesprotéines à action insecticide
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 16 , Issue 2 , June 1995 , pp. 171 - 176
Copyright
Copyright © ICIPE 1995

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