Hostname: page-component-cd9895bd7-p9bg8 Total loading time: 0 Render date: 2024-12-26T17:12:09.041Z Has data issue: false hasContentIssue false
  • English
  • Français

A comparative study of haemocytes in three cyclorrhaphous dipteran flies

Published online by Cambridge University Press:  15 August 2014

Ruchita Pal  and
Krishna Kumar
Ruchita Pal
Affiliation:
Department of Zoology, University of Allahabad, Allahabad211002, India
Krishna Kumar*
Affiliation:
Department of Zoology, University of Allahabad, Allahabad211002, India
*
*E-mail: [email protected]
Get access

Abstract

The larval haemolymph of three cyclorrhaphous dipteran flies, Sarcophaga ruficornis Fab., Musca domestica L. and Chrysomya megacephala Fab., possesses five different types of haemocytes: prohaemocytes; plasmatocytes; granulocytes; spherulocytes; oenocytoids. In addition, vermicytes are found in the haemolymph of S.ruficornis and adipohaemocytes in the haemolymph of M. domestica. The total haemocyte counts in the three dipteran flies show an increasing trend throughout the larval stage, attaining a peak value in the freshly formed white puparium stage and declining thereafter. The differential haemocyte count shows a decline in the number of prohaemocytes with an increase in the number of plasmatocytes, granulocytes and spherulocytes.

Keywords

haemocytesTHCDHCSarcophaga ruficornisChrysomya megacephalaMusca domestica
Type
Research Papers
Information
International Journal of Tropical Insect Science , Volume 34 , Issue 3 , September 2014 , pp. 207 - 216
Copyright
Copyright © ICIPE 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Akai, H. and Sato, S. (1971) An ultrastructural study of the haemopoietic organs of the silkworm, Bombyx mori . Journal of Insect Physiology 17, 1665–1676.Google Scholar
Araujo, H. C. R., Cavalcanti, M. G. S., Santos, S. S., Alves, L. C. and Brayner, F. A. (2008) Hemocytes ultrastructure of Aedes aegypti (Diptera: Culicidae). Micron 39, 184–189.Google Scholar
Arnold, J. W. (1952) The hemocytes of the Mediterranean flour moth, Ephestia kuhniella Zell. (Lepidoptera: Pyralidae). Canadian Journal of Zoology 30, 352–364.CrossRefGoogle Scholar
Arnold, J. W. and Hinks, C. F. (1976) Hemopoiesis in Lepidoptera. The multiplication of circulating hemocytes. Canadian Journal of Zoology 54, 1003–1012.Google Scholar
Arnold, J. W. and Hinks, C. F. (1979) Insect hemocytes under light microscopy: techniques, pp. 531–538. In Insect Hemocytes: Development, Forms, Functions and Techniques (edited by Gupta, A. P.). Cambridge University Press, Cambridge.Google Scholar
Beaulaton, J. and Monpeyssin, M. (1976) Ultrastructure et cytochimie des hemocytés d' Antheraea pernyi Guer (Lepidoptera, Attacidae) au cours du cinquième âge larvaire. I. Prohemocytes, plasmatocytes et granulocytes. Journal of Ultrastructure Research 55, 143–156.CrossRefGoogle ScholarPubMed
Brayner, F. A., Araujo, H. R. C., Cavalcanti, M. G. S., Alves, L. C. and Peixoto, C. A. (2005) Ultrastructural characterization of the hemocytes of Culex quinquefasciatus (Diptera: Culicidae). Micron 36, 359–367.CrossRefGoogle ScholarPubMed
Brayner, F. A., Araujo, H. R. C., Santos, S. S., Cavalcanti, M. G. S., Alves, L. C., Souza, J. R. B. and Peixoto, C. A. (2007) Haemocyte population and ultrastructural changes during the immune response of the mosquito Culex quinquefasciatus to microfilariae of Wuchereria bancrofti . Medical and Veterinary Entomology 21, 112–120.Google Scholar
Brehelin, M. (1982) Comparative study of structure and function of blood cells from two Drosophila species. Cell and Tissue Research 221, 607–615.CrossRefGoogle ScholarPubMed
Castillo, J. C., Robertson, A. E. and Strand, M. R. (2006) Characterization of hemocytes from the mosquitoes Anopheles gambiae and Aedes aegypti . Insect Biochemistry and Molecular Biology 36, 891–903.CrossRefGoogle ScholarPubMed
Chapman, R. F. (2008) The Insects: Structure and Function. Cambridge University Press, Cambridge. 770 pp.Google Scholar
Costa, S. C. P., Ribeiro, C., Girard, P. A., Zumbihl, R. and Brehelin, M. (2005) Modes of phagocytosis of Gram-positive and Gram-negative bacteria by Spodoptera littoralis granular haemocytes. Journal of Insect Physiology 51, 39–46.CrossRefGoogle ScholarPubMed
Essawy, M. (1985) Relations cytophysiologues entre la glande prothoracique et le tissue Sanguin Durant le dernier stade larvaire d'Heliothis armigera (Insecta, Lepidoptera, Noctuidae). Thèse de Doctorat d'Etat, U.S.T.L. Montpellier, France. Google Scholar
Essawy, M., Maleville, A. and Brehelin, M. (1985) The hemocytes of the Heliothis armigera: ultrastructure, functions, and evolution in the course of larval development. Journal of Morphology 186, 255–264.Google Scholar
Essawy, M., Gadelhak, G., El-karaksy, I. and Idriss, M. (1997) Does juvenile hormone regulate haemogram in Bombyx mori L.?, pp. 101–117. In Proceedings of the 1st International Conference of Silk, Cairo, Egypt.Google Scholar
Falleiros, A. M. F., Bombonato, M. T. S. and Gregorio, E. A. (2003) Ultrastructural and quantitative studies of hemocytes in the sugarcane borer, Diatraea saccharalis (Lepidoptera: Pyralidae). Brazilian Archives of Biology and Technology 46, 287–294.CrossRefGoogle Scholar
Franchini, A., Miyan, J. A. and Ottaviani, E. (1996) Induction of ACTH- and TNF-α-like molecules in the hemocytes of Calliphora vomitoria (Insecta, Diptera). Tissue and Cell 28, 587–592.Google Scholar
Gillott, C. (2005) Entomology. Springer. Dordrecht, Netherlands, pp. 515–534.Google Scholar
Giulianini, P. G., Bertolo, F., Battistella, S. and Amirante, G. A. (2003) Ultrastructure of the hemocytes of Cetonischema aeruginosa larvae (Coleoptera, Scarabaeidae): involvement of both granulocytes and oenocytoids in in vivo phagocytosis. Tissue and Cell 35, 243–251.Google Scholar
Graczyk, T. K., Knight, R. and Tamang, L. (2005) Mechanical transmission of human protozoan parasites by insects. Clinical Microbiology Reviews 18, 128–132.Google Scholar
Gupta, A. P. (1979) Hemocyte types: their structures, synonymies, interrelationships, and taxonomic significance, pp. 85–127. In Insect Hemocytes (edited by Gupta, A. P.). Cambridge University Press, Cambridge.Google Scholar
Gupta, A. P. (1985) Cellular elements in the hemolymph, pp. 401–451. In Comprehensive Insect Physiology, Biochemistry and Pharmacology (edited by Kerkut, G. A. and Gilbert, L. I.). Pergamon Press, Oxford.Google Scholar
Gupta, A. P. and Sutherland, D. J. (1966) In vitro transformations of the insect plasmatocyte in some insects. Journal of Insect Physiology 12, 1369–1375.Google Scholar
Hillyer, J. F. and Christensen, B. M. (2002) Characterization of hemocytes from the yellow fever mosquito, Aedes aegypti . Histochemistry and Cell Biology 117, 431–440.Google Scholar
Hillyer, J. F., Schmidt, S. L. and Christensen, B. M. (2003) Hemocyte-mediated phagocytosis and melanization in the mosquito Armigeres subalbatus following immune challenge by bacteria. Cell and Tissue Research 313, 117–127.Google Scholar
Jalali, J. and Salehi, R. (2008) The hemocyte types, differential and total count in Papilio demoleus L. (Lepidoptera: Papilionidae) during post-embryonic development. Munis Entomology and Zoology 3, 199–216.Google Scholar
Jones, J. C. (1956) The hemocytes of Sarcophaga bullata Parker. Journal of Morphology 99, 233–257.Google Scholar
Jones, J. C. (1962) Current concepts concerning insect hemocytes. American Zoologist 2, 209–246.Google Scholar
Kaaya, G. P. and Ratcliffe, N. A. (1982) Comparative study of hemocytes and associated cells of some medically important dipterans. Journal of Morphology 173, 351–365.CrossRefGoogle ScholarPubMed
Lavine, M. D. and Strand, M. R. (2002) Insect hemocytes and their role in immunity. Insect Biochemistry and Molecular Biology 32, 1295–1309.CrossRefGoogle ScholarPubMed
Meister, M. and Lagueux, M. (2003) Drosophila blood cells. Cellular Microbiology 5, 573–580.Google Scholar
Nardi, J. B., Gao, C. and Kanost, M. R. (2001) The extracellular matrix protein lacunin is expressed by a subset of hemocytes involved in basal lamina morphogenesis. Journal of Insect Physiology 47, 997–1006.Google Scholar
Pech, L. L. and Strand, M. R. (2000) Plasmatocytes from the moth Pseudoplusia includens induce apoptosis of granular cells. Journal of Insect Physiology 46, 1565–1573.CrossRefGoogle ScholarPubMed
Ribeiro, C. and Brehelin, M. (2006) Insect haemocytes: what type of cell is that? Journal of Insect Physiology 52, 417–429.Google Scholar
Rivers, D. B., Ruggiero, L. and Hayes, M. (2002) The ectoparasitic wasp Nasonia vitripennis (Walker) (Hymenoptera: Pteromalidae) differentially affects cells mediating the immune response of its flesh fly host, Sarcophaga bullata Parker (Diptera: Sarcophagidae). Journal of Insect Physiology 48, 1053–1064.Google Scholar
Schmidt, O., Theopold, U. and Strand, M. R. (2001) Innate immunity and its evasion and suppression by hymenopteran endoparasitoids. BioEssays 23, 344–351.Google Scholar
Silva, J. E. B., Boleli, I. C. and Simoes, Z. L. P. (2002) Hemocyte types and total and differential counts in unparasitized and parasitized Anastrepha obliqua (Diptera, Tephritidae) larvae. Brazilian Journal of Biology 62, 689–699.Google Scholar
Sohi, S. S. (1971) In vitro cultivation of hemocytes of Malacosoma disstria Hübner (Lepidoptera: Lasiocampidae). Canadian Journal of Zoology 49, 1355–1358.Google Scholar
Srivastava, U. S. and Varma, P. (1979) Mitosis in the haemocytes of Sarcophaga ruficornis (Diptera). Experientia 35, 1457.Google Scholar
Sukontason, K. L., Bunchoo, M., Khantawa, B., Piangjai, S., Rongsriyam, Y. and Sukontason, K. (2007) Comparison between Musca domestica and Chrysomya megacephala as carriers of bacteria in northern Thailand. Southeast Asian Journal of Tropical Medicine and Public Health 38, 38–44.Google ScholarPubMed
Tepass, U., Fessler, L. I., Aziz, A. and Hartenstein, V. (1994) Embryonic origin of hemocytes and their relationship to cell death in Drosophila . Development 120, 1829–1837.Google Scholar
Tojo, S., Naganuma, F., Arakawa, K. and Yokoo, S. (2000) Involvement of both granular cells and plasmatocytes in phagocytic reactions in the greater wax moth, Galleria mellonella . Journal of Insect Physiology 46, 1129–1135.Google Scholar
Yamashita, M. and Iwabuchi, K. (2001) Bombyx mori prohemocyte division and differentiation in individual microcultures. Journal of Insect Physiology 47, 325–331.Google Scholar
Zachary, D. and Hoffmann, J. A. (1973) The haemocytes of Calliphora erythrocephala (Meig.) (Diptera). Zeitschrift für Zellforschung und Mikroskopische Anatomie 141, 55–73.Google Scholar