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Variation in the cuticular hydrocarbons of larvae of Anopheles gambiae and A. arabiensis

Published online by Cambridge University Press:  19 September 2011

Greg I. Anyanwu ,
Angela Phillips  and
David H. Molyneux
Greg I. Anyanwu
Affiliation:
Department of Biological Sciences, University of Salford, Salford M5 4WT, UK
Angela Phillips
Affiliation:
Department of Biological Sciences, University of Salford, Salford M5 4WT, UK
David H. Molyneux
Affiliation:
Department of Biological Sciences, University of Salford, Salford M5 4WT, UK
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Abstract

Gas Liquid Chromatography was used to obtain profiles of cuticular hydrocarbons from single larvae of two species belonging to the Anopheles gambiae complex: A. gambiae and A. arabiensis. By applying discriminant analysis of hydrocarbons it was possible to separate the two populations with a 95% success rate.

The same analysis was used to achieve an average of 94% segregation among their strains. This analysis does not require preservation of specimen by liquid nitrogen or refrigeration and further identification by other procedures can be carried out on the specimens after cuticular hydrocarbon analysis. More importantly the technique permits analysis of single rather than pooled specimens used by earlier investigators.

Résumé

Les profils de hydrocarbones de la cuticle des larves individuelles de deux espéces appartenant au complex Anopheles gambiae (A. gambiae et A. arabiensis) furent obtenus grâce á la chromatographie á phase gaseuse. En utilisant l'analyse du discriminant aux hydrocarbones il a étè possible de separer les deux populations avec un taux de succes de 95%. La même analyse a étè utilisé pour atteindre une moyenne de 94% de separation entre ces espéces. L'analyse ne necessite pas la preservation des specimen dans du nitrogene ou dans le frigidaire et d'autre procedures d'identifïcation peuvent être effectuer après l'analyse des hydrocarbones. Plus important, la technique permet l'analyse d'un seul specimen au lieu de plusieurs comme precedament.

Keywords

Anopheles gambiaeA. arabiensislarvaecuticular hydrocarbon analysisAnopheles gambiaeA. arabiensislarvesanalyse des hydrocarbones de cuticleclassification
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 15 , Issue 2 , April 1994 , pp. 117 - 122
Copyright
Copyright © ICIPE 1994

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References

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