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Salivary glands and their digestive enzymes in pod-sucking bugs (Hemiptera: Coreoidea) associated with cowpea Vigna unguiculata ssp. unguiculata in Nigeria

Published online by Cambridge University Press:  01 March 2007

O.L. Soyelu ,
A.E. Akingbohungbe  and
R.E. Okonji
O.L. Soyelu*
Affiliation:
Department of Plant Science, Faculty of Agriculture, Obafemi Awolowo University, Ile-Ife, Nigeria
A.E. Akingbohungbe
Affiliation:
Department of Plant Science, Faculty of Agriculture, Obafemi Awolowo University, Ile-Ife, Nigeria
R.E. Okonji
Affiliation:
Department of Biochemistry, Obafemi Awolowo University, Ile-Ife, Nigeria
*
*E-mail: [email protected]
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Abstract

The salivary glands of the cowpea pod-sucking bugs Anoplocnemis curvipes (Fabricius), Clavigralla tomentosicollis Stål, Clavigralla shadabi Dolling, Riptortus dentipes (Fabricius) and Mirperus jaculus (Thunberg) are described and illustrated. Extracts of the glands were assayed for the presence of proteinases, α-amylase, β-amylase and amyloglucosidase. The salivary glands consist of four-lobed principal glands and a tubular accessory gland, and they differ in shape and size among the different species except for the median lobe, which is more or less spherical. The sickle-shaped nature of the posterior lobe reported before for Riptortus linearis (Linnaeus) was also observed in R. dentipes, suggestive of a probable generic characteristic. Anoplocnemis curvipes has the biggest salivary gland, and it is followed by R. dentipes, M. jaculus, C. tomentosicollis and C. shadabi in descending order. Assays of the gland extracts revealed that the four digestive enzymes tested are present in the five coreoid species. Proteinases were preponderant (1.4–94.9 μg/ml), while amylases were detected in traces [(0.5–11.0) × 10− 3 μg/ml]. Proteinases may be largely responsible for the feeding damage caused by the coreoid species and their concentration in the glands of the different species correlates well with their known capacity to inflict pod damage and yield reduction in cowpea. Fourth instar nymphs of the different species also had higher concentrations of the digestive enzymes compared with their respective adults; and this correlates with the known ability of the nymphs to cause greater pod damage and yield reduction than adults.

Keywords

Anoplocnemis curvipesRiptortus dentipesMirperus jaculusClavigralla tomentosicollisClavigralla shadabisalivary glandsproteinasesα-amylaseβ-amylaseamyloglucosidase
Type
Research Paper
Information
International Journal of Tropical Insect Science , Volume 27 , Issue 1 , March 2007 , pp. 40 - 47
Copyright
Copyright © ICIPE 2007

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References

Abiose, S. H., Atalabi, T. A. and Ajayi, L. O. (1988) Fermentation of African locust beans: Microbiological and biochemical studies. Nigerian Journal of Biological Sciences 1, 103–117.Google Scholar
Cohen, A. C. (1993) Organization of digestion and preliminary characterization of salivary trypsin-like enzymes in a predaceous heteropteran, Zelus renardii. Journal of Insect Physiology 39, 823–829.CrossRefGoogle Scholar
Cohen, A. C. and Wheeler, A. G. (1998) Role of saliva in the highly destructive four-lined plant bug (Hemiptera: Miridae: Mirinae). Annals of the Entomological Society of America 91, 94–100.CrossRefGoogle Scholar
Fuwa, H. (1954) A simple new method for microdetermination of amylase by the use of amylose as the substrate. Journal of Biochemistry 41, 583–603.CrossRefGoogle Scholar
Hehre, E. J., Brewer, C. F. and Genghof, D. S. (1979) Scope and mechanism of carbohydrase action: Hydrolytic and non-hydrolytic actions of β-amylase on α- and β-maltosyl fluoride. Journal of Biological Chemistry 254, 5942–5950.CrossRefGoogle Scholar
Hori, K. (1972) Comparative study of a property of salivary amylase among various heteropterous insects. Comparative Biochemistry and Physiology 42B, 501–508.Google Scholar
Hori, K. (1975) Amino acids in the salivary glands of the bugs, Lygus disponsi and Eurydema rugosum. Insect Biochemistry 5, 165–169.CrossRefGoogle Scholar
Jackai, L. E. N. and Daoust, R. A. (1986) Insect pests of cowpeas. Annual Review of Entomology 31, 95–119.CrossRefGoogle Scholar
Jackai, L. E. N., Singh, S. R., Raheja, A. K. and Wiedijk, F. (1985) Recent trends in the control of cowpea pests in Africa, pp. 233–245. In Cowpea Research, Production and Utilization (Edited by Singh, S. R. and Rachie, K. O.). John Wiley and Sons, Chichester.Google Scholar
Konno, K., Hirayama, C. and Shinbo, H. (1997) Glycine in digestive juice: A strategy of herbivorous insects against chemical defense of host plants. Journal of Insect Physiology 43, 217–224.CrossRefGoogle Scholar
Lawrence, P. K. and Koundal, K. R. (2002) Plant protease inhibitors in control of phytophagous insects. Electronic Journal of Biotechnology: EJB 5, 93–109. http://www.ejbiotechnology.info/content/vol5/issue1/full/3/3.pdf.CrossRefGoogle Scholar
Lecuona-Villanueva, A., Torruco-Uco, J., Chel-Guerrero, L. and Betancur-Ancona, D. (2006) Physicochemical characterization of Mexican cowpea (Vigna unguiculata) tailing starch. Stärke 58, 25–34.CrossRefGoogle Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Machuka, J. (2002) Potential role of transgenic approaches in the control of cowpea insect pests, pp. 213–222. In Challenges and Opportunities for Enhancing Sustainable Cowpea Production. Proceedings of the World Cowpea Conference III. 4–8 September 2000 (Edited by Fatokun, C. A., Tarawali, S. A., Singh, B. B., Kormawa, P. M. and Tamo, M.). IITA, Ibadan, Nigeria.Google Scholar
Machuka, J. S., Okeola, O. G., Van Damme, E. J. M., Chrispeels, M. J., Van Leuven, F. and Peumans, W. J. (1999) Isolation and partial characterization of galactose-specific lectins from African yam beans, Sphenostylis stenocarpa Harms. Phytochemistry 51, 721–728.CrossRefGoogle Scholar
Miles, P. W. (1972) The saliva of Hemiptera. Advances in Insect Physiology 9, 183–255.CrossRefGoogle Scholar
Mitchell, P. L. (2000) Leaf-footed bugs (Coreidae), pp. 337–383. In Heteroptera of Economic Importance (Edited by Schaefer, C. W. and Panizzi, A. R.). CRC Press, Boca Raton, Florida.CrossRefGoogle Scholar
Morihara, K. and Tsuzuki, H. (1977) Production of protease and elastase by Pseudomonas aeruginosa strains isolated from patients. Infection and Immunity 15, 679–685.CrossRefGoogle ScholarPubMed
Murdock, L. L., Brookhart, G., Dunn, P. E., Foard, D. E. and Kelley, S. (1987) Cysteine digestive proteinases in Coleoptera. Comparative Biochemistry and Physiology 87B, 783–787.Google Scholar
Nuorteva, P. (1956) Studies on the comparative anatomy of the salivary glands in four families of Heteroptera. Annales Entomologica Fennica 22, 45–54.Google Scholar
Omitogun, O. G., Jackai, L. E. N. and Thottappilly, G. (1999) Isolation of insecticidal lectin-enriched extracts from African yam bean (Sphenostylis stenocarpa) and other legume species. Entomologia Experimentalis et Applicata 90, 301–311.CrossRefGoogle Scholar
Panizzi, A. R., Schaefer, C. W. and Natuhara, Y. (2000) Broad-headed bugs (Alydidae), pp. 321–336. In Heteroptera of Economic Importance (Edited by Schaefer, C. W. and Panizzi, A. R.). CRC Press, Boca Raton, Florida.Google Scholar
Paselk, R. A. (2001) Carbohydrates. Chem 431 Lecture Notes 28. Department of Chemistry, Humboldt State University. http://www.humboldt.edu/~rap1/C431.F01/C431Notes/C431n01oct.htm.Google Scholar
Pollard, D. G. (1973) Plant penetration by feeding aphids (Hemiptera Aphidoidea): A review. Bulletin of Entomological Research 62, 631–714.CrossRefGoogle Scholar
Ryan, C. A. (1990) Protease inhibitors in plants: Genes for improving defenses against insects and pathogens. Annual Review of Phytopathology 28, 425–449.CrossRefGoogle Scholar
Schaefer, C. W. and Mitchell, P. L. (1983) Food plants of the Coreoidea (Hemiptera: Heteroptera). Annals of the Entomological Society of America 76, 591–615.CrossRefGoogle Scholar
Singh, S. R. and van Emden, H. F. (1979) Insect pests of grain legumes. Annual Review of Entomology 24, 255–278.CrossRefGoogle Scholar
Singh, S. R., Jackai, L. E. N., Santos, J. H. R. and Adalla, C. B. (1986) Insect pests of cowpea, pp. 43–89. In Insect Pests of Tropical Food Legumes (Edited by Singh, S. R.). John Wiley and Sons, Chichester.Google Scholar
Somogyi, M. (1945) A new reagent for the determination of sugars. Journal of Biological Chemistry 160, 61–68.CrossRefGoogle Scholar
Wheeler, A. G. Jr. and Miller, G. L. (1981) Fourlined plant bug (Hemiptera: Miridae), a reappraisal: Life history, host plants, and response to feeding. Great Lakes Entomology 14, 23–35.Google Scholar