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Rapid changes in the reproductive cycle of wild-caught tsetse, Glossina pallidipes Austen, when brought into the laboratory

Published online by Cambridge University Press:  19 September 2011

Sarah E. Randolph
Affiliation:
Department of Zoology, South Parks Road, Oxford OX1 3PS, England
David J. Rogers
Affiliation:
Department of Zoology, South Parks Road, Oxford OX1 3PS, England
John Kiilu
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi, Kenya
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Abstract

This paper describes differences in reproductive and nutritional conditions that develop within 2 weeks of the transfer of wild-caught female Glossina pallidipes Austen to apparently ideal laboratory conditions. In order to investigate the relative sampling efficiency of baited NG2B traps for female G. pallidipes and the flies' changing feeding behaviour on different days of the pregnancy cycle, samples of female G. pallidipes taken directly from the field and those held in the laboratory for a known number of days after larviposition were subjected to both ovarian dissection (including the measurement of the uterine content and the two largest ovarioles) and fat and haematin analysis. Under the laboratory conditions of slightly lower mean temperature (buffered against diurnal fluctuations), lower activity and higher feeding frequency than in the field, the interlarval period was prolonged, the third larval instar was disproportionately prolonged, the ovarioles and larvae in utero showed differential growth rates, pupal size was increased and the fat reserves of post-partum females was depleted. It is concluded that tsetse are very responsive to the precise environmental conditions in which they find themselves and that absolute quantitative measurements made in the laboratory cannot be applied directly to the field situation.

Résumé

Cette thèse décrit les différences en matière des conditions reproductives et nutritionnelles qui se dévéloppent dans l'espace de deux semaines d'adaptation de la femelle sauvage capturée de tsé-tsé Glossina pallidipes d'Austen aux conditions apparemment idéales dans un laboratoire. Dans le but d'investiguer l'efficacité relative d'échantillonnage des pièges NG2B où I'on a mis de appâts pour attraper des femelles G. pallidipes et de faire des recherches sur le changement du comportement alimentaire des mouches au cours du cycle de “grossesse”, des échantillons de la femelle G. pallidipes pris directement de leur champ naturel et ceux de celles qui se trouvent dans le laboratoire pendant un nombre jours connus après le dépot de larves ont été soumises à la dissection de l'ovaires (comprenant le mesurage du contenu utéin et les tubes ovaires les plus grands) et à l'analyse des matiére grasses et de l'hématine. Sous les conditions Iaboratoires d'une température légèrement basse (protégée contre les fluctuations diurnes) une basses activité et une fréquence alimentaire plus élévée que dans la nature, la période interlavaire était prolongée, les tubes ovaires et les larves in utero ont montré des taux grasses des femelles dans la période post-natale était épuisées. On est arrivée à la conclusion selon laquelle les tsé-tsé sont très sensibles aux conditions environnementales précises dans lesquelles ils se trouvent et que les mesures quantitatives absolues prises dans le laboratoire ne peuvent pas etre appliquées directement dans un environnement naturel.

Type
Research Article
Copyright
Copyright © ICIPE 1990

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References

REFERENCES

Brightwell, R., Dransfield, R. D. and Kyorku, C. (1991) Improvement of a low-cost trapping technology for tsetse flies Glossina pallidipes and G. longipennis. Med. Vet. Entomol. (in press).Google Scholar
Challier, A. (1965) Amélioration de la méthode de determination de l'âge physiologique des glossines. Études faites sur Glossina palpalis gambiensis Vanderplank, 1949. Bull. Soc. Path, exot. 58, 250259.Google Scholar
Challier, A. (1973) Ecologie de Glossina palpalis gambiensis Vanderplank, 1949 (Diptera-Muscidae) en savanne d'Afrique Occidentale. Mem. ORSTOM, 64, 274 pp.Google Scholar
Denlinger, D. L. and Ma, W.-C. (1974) Dynamics of the pregnancy cycle in the tsetse Glossina morsitans. J. Insect Physiol. 20, 10151026.Google Scholar
Dransfield, R. D., Brightwell, R., Kiilu, J., Chaudhury, M. F. and Adabie, D. A. (1989) Size and mortality rates of Glossina pallidipes in the semiarid zone of southwest Kenya. Med. Vet. Entomol. 3, 8395.CrossRefGoogle Scholar
Ford, J., Maudlin, I. and Humphryes, K. C. (1972) Comparisons between three small collections of Glossina morsitans morsitans (Machado) (Diptera: Glossinidae) from the Kilombero River Valley, Tanzania. Part 1. Characteristics of flies exhibiting different patterns of behaviour. Acta Trop. 29, 231249.Google Scholar
Harley, J. M. B. (1968) The influence of temperature on reproduction and development in four species of Glossina (Diptera: Muscidae). Proc. R. Entomol. Soc. London (A) 43, 170177.Google Scholar
Jordan, A. M., Trewern, M. A., Southern, D. I., Pell, P. E. and Davies, E. D. G. (1977) Differences in laboratory performance between strains of Glossina morsitans morsitans Westwood from Rhodesia and Tanzania and associated chromosome diversity. Bull, entomol. Res. 57, 3548.Google Scholar
Langley, P. A. (1977) Physiology of tsetse flies (Glossina spp.) (Diptera: Glossinidae): a review. Bull, entomol. Res. 67, 523574.Google Scholar
Randolph, S. E. and Rogers, D. J. (1986) The use of discriminant analysis for the estimation of sampling biases for female tsetse. Ecol. Entomol. 11, 205220.CrossRefGoogle Scholar
Randolph, S. E., Rogers, D. J. and Kiilu, J. (1991) The feeding behaviour, activity and trappability of wild female Glossina pallidipes Austen in relation to their pregnancy cycle. Med. Vet. Entomol. (in press).CrossRefGoogle Scholar
Rogers, D. J. and Randolph, S. E. (1978) Metabolic strategies of male and female tsetse (Diptera:Glossinidae) in the field. Bull, entomol. Res. 68, 639654.Google Scholar
Taylor, P. (1976) Blood-meal size of Glossina morsitans Westw. and G. pallidipes Austen (Diptera: Glossinidae) under field conditions. Trans. Rhod. Sci. Assoc. 57, 2934.Google Scholar
Vale, G. A. (1971) Artificial refuges for tsetse (Glossina spp.). Bull, entomol. Res. 61, 331350.Google Scholar