Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-28T01:09:11.776Z Has data issue: false hasContentIssue false
  • English
  • Français

Localization and characterization of salivary gland cholinesterase in the tsetse Glossina morsitans (Diptera: Glossinidae)

Published online by Cambridge University Press:  19 September 2011

Thomas K. Golder  and
Nilu Y. Patel
Thomas K. Golder
Affiliation:
International Centre of Insect Physiology and Ecology, P.O. Box 30772, Nairobi, Kenya
Nilu Y. Patel
Affiliation:
International Centre of Insect Physiology and Ecology, P.O. Box 30772, Nairobi, Kenya
Get access

Abstract

The salivary gland of Glossina morsitans contains cholinesterase activity which is distributed in a network surrounding each epithelial cell of the secretory region. The enzyme does not appear to be associated with any neural structures and is similar to cholinesterases of most insects in its reaction with inhibitors and in its ability to hydrolyze a wide range of substrates.

Keywords

Cholinesterasesalivary glandstsetsesubstratesinhibitor
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 3 , Issue 2-3 , September 1982 , pp. 167 - 171
Copyright
Copyright © ICIPE 1982

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bartos, E. M. and Glinos, A. D. (1976) Properties of growth related acetylcholinesterase in a cell line of fibroblast origin, J. Cell Biol. 69, 638–646.CrossRefGoogle Scholar
Blackburn, K., Wilson, B. W. and Vellis, J. de (1974) Synthesis and release of AChE by cultured chick nerve. In Vitro 10, 372.Google Scholar
Chadwick, L. E. (1963) Actions on insects and other invertebrates. In Cholinesterases and Anticholinesterase Agents (Ed. by Koelle, G. B.), pp. 741–748. Springer, Berlin.CrossRefGoogle Scholar
Chubb, I. W., Goodman, S. and Smith, A. D. (1976) Is acetylcholinesterase secreted from central neurons into the cerebrospinal fluid? Neuroscience 1, 57–62.CrossRefGoogle Scholar
Chubb, I. W. and Smith, A. D. (1974) Release of acetylcholinesterase into perfusate from the ox adrenal gland. Proc. R. Soc. 191, 263–270.Google Scholar
Davis, B. J. (1964) Disc electrophoresis—II. Method and application to human serum proteins. Ann. N.Y. Acad. Sci. 121, 305–650.CrossRefGoogle ScholarPubMed
Deak, I. I. (1977) A histochemical study of the muscles of Drosophila melanogaster. J. morph. 153, 307–316.CrossRefGoogle ScholarPubMed
Frontali, N., Piazza, R. and Scopelleti, R. (1971) Localization of acetylcholinesterase in the brain of Periplaneta americana. J. Insect Physiol. 17, 1833–1842.CrossRefGoogle ScholarPubMed
Golder, T. K., Neiberg, P. S. and Wilson, B. W. (1977) Ultrastructural localization of acetylcholinesterase in cultured cells II. Cyclohexamide-treated embryo muscle. J. Histochem. Cytochem. 25, 376–383.CrossRefGoogle Scholar
Golder, T. K., Neiberg, P. S. and Wilson, B. W. (1978) Ultrastructural localization of acetylcholinesterase in cultured cells III. DFP treated embryo muscle. J. Histochem. Cytochem. 26, 719–728.CrossRefGoogle ScholarPubMed
Hess, A. (1972) Histochemical localization of cholinesterase in the brain of the cockroach (Periplaneta americana). Brain Res. 46, 287–295.CrossRefGoogle ScholarPubMed
Kapin, M. A. and Ahmad, S. (1980) Esterases in the larval tissues of the gypsy moth, Lymantria dispar (L): optimum assay conditions, quantification and characterization. Insect Biochem. 10, 331–337.CrossRefGoogle Scholar
Karnovsky, M. J. and Roots, L. (1964) A ‘direct-coloring’ thiocholine method for cholinesterase. J. Histochem. Cytochem. 12, 219–221.CrossRefGoogle Scholar
Koelle, G. B. and Friedenwald, J. S. (1949) A histochemical method for localizing cholinesterase activity. Proc. Soc: exp. Biol. Med. 70, 617–622.Google ScholarPubMed
Landolt, A. M. and Sandri, C. (1966) Cholinergische Synapsen in Obserschlundganglion der Waldameise. Z. Zellforsch. mikrosk. Anat. 69, 246–259.CrossRefGoogle Scholar
Neal, H. (1975) Effects of I.-glutamate and other drugs on some membrane properties of the muscle fibres of Diptera. J. Insect Physiol. 21, 1771–1778.CrossRefGoogle Scholar
Sawyer, H. R., Golder, T. K., Neiberg, P. S. and Wilson, B. W. (1976) Ultrastructural localization of acetylcholinesterase in cultured cells. I. Embryo muscle. J. Histochem. Cytochem 24, 969–978.CrossRefGoogle ScholarPubMed
Sequeira, M. L. (1971) Structure and functional relations of the salivary glands of the tsetse fly. MSc thesis. University of Nairobi.Google Scholar
Smith, D. S. and Trehern, J. E. (1965) The electron microscopic localization of cholinesterase activity in the central nervous system of an insect, Periplaneta americana L. J. Cell Biol. 26, 445–465.CrossRefGoogle Scholar
Somogyi, P., Chubb, I. W. and Smith, A. D. (1975) A possible structural basis for the extracellular release of acetylcholinesterase. Proc R. Soc. 191, 271–283.Google ScholarPubMed
Whitemore, E. and Gilbert, L. I. (1974) Haemolymph proteins and lipoproteins in Lepidoptera. A comparative electrophoretic study. Comp. Biochem. Physiol. 47B, 63–78.Google Scholar
Wigglesworth, V. B. (1958) The distribution of esterase in the nervous system and other tissues of the insect Rhodnius prolixus. Quart. J. Micros. Sci. 99, 441–450.Google Scholar
Wood, E., Zerba, E., Picollo, M. and Licastro, S. de (1979) Partial purification and characterization of Triatoma infestans head acetylcholinesterase. Insect Biochem. 9, 595–601.CrossRefGoogle Scholar