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Larvicidal activity of the α-amylase inhibitor from the seeds of Macrotyloma uniflorum (Leguminosae) against Aedes aegypti (Diptera: Culicidae)

Published online by Cambridge University Press:  10 May 2011

Laxmi Gupta ,
Sudhakar Deshpande ,
Vrushali Tare  and
Sushma Sabharwal
Laxmi Gupta
Affiliation:
Department of Chemistry, Division of Biochemistry, University of Pune, Ganeshkhind Road, Pune411 007, India
Sudhakar Deshpande
Affiliation:
Entomology Section, National Chemical Laboratory, Pune411 008, India
Vrushali Tare
Affiliation:
Entomology Section, National Chemical Laboratory, Pune411 008, India
Sushma Sabharwal*
Affiliation:
Department of Chemistry, Division of Biochemistry, University of Pune, Ganeshkhind Road, Pune411 007, India
*
*E-mail: [email protected]
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Abstract

The objective of the present study was to evaluate the larvicidal potential of the α-amylase inhibitor from the seeds of Macrotyloma uniflorum (Lam.) Verdc. against Aedes aegypti (L.) larvae. Macrotyloma uniflorum α-amylase inhibitor (MUAI) was partially purified using ion exchange chromatography on a carboxymethyl cellulose column. MUAI at concentrations of 20, 60, 100 and 200 ppm was tested against different instar larvae, pupae and adults of A. aegypti to establish its bioefficacy. MUAI showed a strong larvicidal and a moderate adulticidal effect at a concentration of 200 ppm but did not exhibit pupicidal and ovicidal activities. MUAI-treated larvae showed lower α-amylase activity. MUAI also exhibited ovipositional deterrence activity. Dose-dependent mortality was observed, being highest in the first and second instar larvae. The results thus obtained provide a safe and eco-friendly means in the biological control of A. aegypti larvae.

Keywords

adulticidalAedes aegyptilarvicidalMacrotyloma uniflorum α-amylase inhibitoroviposition deterrence
Type
Research Paper
Information
International Journal of Tropical Insect Science , Volume 31 , Issue 1-2 , June 2011 , pp. 69 - 74
Copyright
Copyright © ICIPE 2011

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