Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-28T02:27:14.309Z Has data issue: false hasContentIssue false
  • English
  • Français

A laboratory technique for infecting adult tsetse with a fungal pathogen

Published online by Cambridge University Press:  19 September 2011

Nguya K. Maniania
Nguya K. Maniania
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
Get access

Abstract

A technique for infecting adult tsetse with an entomopathogenic fungus was developed and tested in the laboratory. A nitrocellulose filter membrane (Millipore) was used as substrate for retaining conidia after fitration, and a cylindrical plastic tube served as a chamber for the flies. The technique was designed to allow accurate estimation of inoculum on the substrate, thereby permitting comparison of virulence during the screening of fungal pathogens against tsetse flies. Glossina morsitans centralis adults were susceptible to Metarhizium anisopliae at all doses tested, and mortalities were dose dependent. No significant differences in mortalities were observed when flies were exposed for different durations to the pathogen. The number of conidia picked up by flies varied considerably with exposure time and concentration.

Résumé

Une technique pour contaminer la mouche tsé-tsé avec un champignon entomopathogène a été mise au point puis testée au laboratoire. Une membrane filtrante en ester de cellulose (Millipore) a été utilisée comme substrat pour retenir les conidies après filtration, tandis qu'un tube cylindrique en plastique a servi pour chambre d'élevage pour les mouches. La technique a été conçue pour une estimation correcte de l'inoculum sur le substrat, ainsi permettant une comparaison de virulence entre les souches fongiques pour la lutte biologique contre la mouche tsé-tsé. Les adultes de Glossina morsitans centralis étaient susceptibles au Metarhizium anisopliae à toutes les doses éprouvées, et la mortalité était dose dépendante. La mortalité n'était pas significativement différente lorsque les mouches étaient exposées à l'inoculum pendant un temps variable. Le nombre de conidies collectées par les insectes variait considérablement en fonction du temps d'exposition et la concentration du pathogène.

Keywords

Glossina morsitans centralisMetarhizium anisopliaeinoculation techniquelaboratoryentomopathogenic fungusbiological controlGlossina morsitans centralisMetarhizium anisopliaetechnique d'inoculationlaboratoirechampignon entomopathogènelutte biologique
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 15 , Issue 4-5 , October 1994 , pp. 421 - 426
Copyright
Copyright © ICIPE 1994

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Abbot, W. S. (1925) A method of computing the effectiveness of an insecticide. J. Econ. Entomol. 18, 265–267.CrossRefGoogle Scholar
Clark, B. T., Keller, R. W., Fukula, T. and Lindegren, E. J. (1968) Field and laboratory studies on the pathogenicity of the fungus Beauveria bassiana to three genera of mosquitoes. J. Invertebr. Pathol. 11, 1–7.CrossRefGoogle ScholarPubMed
Fargues, J. and Remaudière, G. (1977) Considerations of the specificity of entomopathogenic fungi. Mycopathologia 62, 31–37.CrossRefGoogle Scholar
Ferron, P., Fargues, J. and Riba, G. (1991) Fungi as microbial insecticides against pests. In Handbook of Applied Mycology (Edited by Arora, D. K., Ajello, L. and Mukerji, K. G.) (Vol. 2, Humans, Animals and Insects), pp. 665–706. Marcel Dekker Inc., New York.Google Scholar
Fuxa, J. R. and Tanada, Y. (1987) Epizootiology of Insect Diseases. John Wiley and Sons, New York.Google Scholar
Hall, M. J. R. and Langley, P. A. (1987) Development of a system for sterilising tsetse flies, Glossina spp., in the field. Med. Vet. Ent. 1, 201–210.CrossRefGoogle ScholarPubMed
Hall, R. A. and Papierok, B. (1982) Fungi as biological control agents of arthropods of agricultural and medical importance. Parasitology 84, 205–240.CrossRefGoogle Scholar
Kaaya, G. P. (1989) Glossina morsitans morsitans: Mortalities caused in adults by experimental infection with entomopathogenic fungi. Acta Tropica 46, 107–114.Google Scholar
Kaaya, G. P. and Okech, M. A. (1990) Horizontal transmission of mycotic infection in adult tsetse, Glossina morsitans morsitans. Entomophaga 35, 589–600.CrossRefGoogle Scholar
Poinar, G. O. Jr, Van der Geest, L., Helle, W. and Wassink, H. (1977) Pathology and nematode parasitism. In The Future for Biological Methods in Integrated Control (Edited by Laird, M.), pp. 75–92. Ottawa, IDRC-077E.Google Scholar
Rizzo, D. C. (1977) Age of three dipteran hosts as a factor governing the pathogenicity of Beauveria bassiana and Metarhizium anisopliae. J. Invertebr. Pathol. 30, 127–130.CrossRefGoogle Scholar
Roberts, D. W. and Campbell, S. (1977) Stability of entomopathogenic fungi. Misc. Pub. Entomol. Soc. Amer. 10, 19–75.Google Scholar
SAS Institute (1985) SAS User's Guide: Statistics. SAS Institute, Cary, N.C.Google Scholar
Schaerffenberg, B. (1964) Biological and environmental conditions for the development of mycoses caused by Beauveria bassiana and Metarhizium. J. Insect Pathol. 6, 8–20.Google Scholar